Integrative Analyses of Long Non-coding RNA and mRNA Involved in Piglet Ileum Immune Response to Clostridium perfringens Type C Infection

Abstract

Long non-coding RNAs (lncRNAs) have been shown to play important roles in regulating host immune and inflammatory responses to bacterial infection. Infection with Clostridium perfringens ( C. perfringens ), a food-borne zoonotic pathogen, can lead to a series of inflammatory diseases in human and piglet, greatly challenging the healthy development of global pig industry. However, the roles of lncRNAs involved in piglet immune response against C. perfringens type C infection remain unknown. In this study, the regulatory functions of ileum lncRNAs and mRNAs were investigated in piglet immune response to C. perfringens type C infection among resistance (IR), susceptibility (IS) and sham-inoculation (control, IC) groups. A total of 480 lncRNAs and 3,669 mRNAs were significantly differentially expressed, the differentially expressed lncRNAs and mRNAs in the IR and IS groups were enriched in various pathways of ABC transporters, olfactory transduction, PPAR signaling pathway, chemokine signaling pathway and Toll-like receptor signaling pathway, involving in regulating piglet immune responses and resistance during infection. There were 212 lncRNAs and 505 target mRNAs found to have important association with C. perfringens infectious diseases, furthermore, 25 dysregulated lncRNAs corresponding to 13 immune-related target mRNAs were identified to play potential roles in defense against bacterial infection. In conclusion, the results improve our understanding on the characteristics of lncRNAs and mRNAs on regulating host immune response against C. perfringens type C infection, which will provide a reference for future research into exploring C. perfringens -related diseases in human.

Integrated analysis of lncRNAs and mRNAs reveals key trans-target genes associated with ETEC-F4ac adhesion phenotype in porcine small intestine epithelial cells

“…We identified 78 lncRNAs and 223 mRNAs that were differentially expressed in the comparison of F4R negative vs. F4R positive small intestine epithelial cells. This is in consistent with Huang et al who reported a similar trend in their study on piglet ileum immune response to C. perfringens type C infection [ 4 ].…”

“…Data available from high-throughput sequencing studies have revealed that only a very small percentage (1–2%) of the mammalian genome encode proteins, but tens of thousands of intergenic sites are transcribed to non-coding RNA [ 3 ]. This transcription plays a critical role in the regulation of gene expression during several biological processes [ 4 ].…”

“…Long non-coding RNAs (lncRNAs) have been implicated in the regulation of host inflammatory response against infections caused by enteropathogenic bacteria [ 9 ]. For instance, Salmonella infection has been demonstrated to be responsible for changes in the expression of certain sensitive lncRNAs in infection in HeLa cells [ 4 ].…”

“…The overexpression of lncRNA TCONS00183659 in resistant piglets has been reported to regulate the expressions of inflammatory factors IFIT2, MX1 and MX2 to improve the resistance against ETEC-F18 infection in weaning piglet [ 4 ]. Similarly, in the ETEC-F18-infected mice diarrhea model, the overexpression of lncRNA ENSMUST00000122226 has been reported to cause improvement in the expression of adhesion molecule CD28 on T cell surface facilitating the secretion of different inflammatory cytokines, including IFN and IL, and thus finally leading to intestinal inflammatory response and diarrhea disease [ 4 ] These studies have collectively highlighted the changes and roles of lncRNAs in regulating host inflammatory responses during bacterial infections,…”

Comparative Analysis of Microbial Community Structure and Function in the Gut of Wild and Captive Amur Tiger

“…We tried to confirm the relationship between gut microbiota and metabolism function; however, it was somewhat unrealistic to treat each taxonomy ID and each function as independent (Hooper et al, 2002) while the taxonomy ID clusters and classifications of KEGG Level2 were closely correlated to each other. By the correlation result, we found that the Firmicutes (Clostridium) in wild tigers was positively correlated with the function of infectious disease, a similar conclusion was reached from another analysis showed that Clostridium perfringens was the second most common bacteria that cause bacterial illnesses (Scharff, 2012;Huang et al, 2019). According to our results, Firmicutes (Blautia) was positively correlated with the immune system.…”