Integration of positional signals and regulation of wing formation and identity by Drosophila vestigial gene

Kim, Jaeseob; Sebring, Angela; Esch, Jeffrey J.; Kraus, Mary Ellen; Vorwerk, Kathy; Magee, Jeffrey C.; Carroll, Sean B.

doi:10.1038/382133a0

Cited by 458 publications

(500 citation statements)

References 48 publications

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“…4,26 However, other investigators have argued that vg does not induce proliferation but only transdetermination. 27,28 To clearly establish the role of vg and sd in proliferation, we overexpressed the Drosophila Dacapo (DAP) protein which shares strong homology with human p21.…”

Section: Resultsmentioning

confidence: 99%

“…3 Several studies point to the vestigial (vg) gene as the main target for the patterning systems defined by the two axes of the wing. [4][5][6] The vg gene encodes a nuclear protein expressed during embryogenesis in the presumptive regions of wing and halter imaginal discs in the central nervous system and in a subset of thoracic muscles. 7 All vg mutants are characterized by a reduced wing phenotype, indicating a requirement for VG in wing development.…”

Section: Introductionmentioning

confidence: 99%

“…The boundary enhancer (vgBE) mediates transcription of vg under the control of the Notch pathway via the binding of the suppressor of hairless (Su(H)) transcription factor. 4,8 The quadrant enhancer (vgQE) directs vg expression under control of the dpp pathway via the binding of the mothers against dpp (MAD) protein. 4,5 In this way, vg integrates inputs from the two axes that pattern wing development.…”

Section: Introductionmentioning

confidence: 99%

“…4,8 The quadrant enhancer (vgQE) directs vg expression under control of the dpp pathway via the binding of the mothers against dpp (MAD) protein. 4,5 In this way, vg integrates inputs from the two axes that pattern wing development. 6,9 It has been shown that vg interacts genetically and molecularly with the scalloped (sd) gene product.…”

Section: Introductionmentioning

confidence: 99%

“…10,12,14 It has been shown that ectopic expression of vg in other imaginal discs induces wing outgrowths, this being dependent on the presence of SD. 4,11 Therefore, vg seems to be required for both wing identity and cell proliferation, suggesting that the VG-SD dimer could be one of the links between signalling genes and genes involved in cell proliferation. 6 We have previously shown a strong interaction between specific antiproliferative drugs, such as methotrexate, aminopterin, fluorodeoxyuridine, and the phenotype of vg mutants.…”

Section: Introductionmentioning

confidence: 99%

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The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

et al. 2003

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Links between genes involved in development, proliferation and apoptosis have been difficult to establish. In the Drosophila wing disc, the vestigial (vg) and the scalloped (sd) gene products dimerize to form a functional transcription factor. Ectopic expression of vg in other imaginal discs induces outgrowth and wing tissue specification. We investigated the role of the VG-SD dimer in proliferation and showed that vg antagonizes the effect of dacapo, the cyclin-cdk inhibitor. Moreover, ectopic vg drives cell cycle progression and in HeLa cultured cells, the VG-SD dimer induces cell proliferation per se. In Drosophila, ectopic vg induces expression of dE2F1 and its targets dRNR2 and string. In addition vg, but not dE2F1, interacts with and induces expression of dihydrofolate reductase (DHFR). Moreover, a decrease in VG or addition of aminopterin, a specific DHFR inhibitor, shift the dorso-ventral boundary cells of the disc to a cell death sensitive state that is correlated with reaper induction and DIAP1 downregulation. This indicates that vg in interaction with dE2F1 and DHFR is a critical player for both cell proliferation and cell survival in the presumptive wing margin area.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

et al. 2003

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Themes from a NASA workshop on gene regulatory processes in development and evolution

Davidson

Ruvkun

1999

J. Exp. Zool.

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A memorable workshop, focused on causal mechanisms in metazoan evolution and sponsored by NASA, was held in early June 1998, at MBL. The workshop was organized by Mike Levine and Eric H. Davidson, and it included the PI and associates from 12 different laboratories, a total of about 30 people. Each laboratory had about two and one half hours in which to represent its recent research and cast up its current ideas for an often intense discussion. In the following we have tried to enunciate some of the major themes that emerged, and to reflect on their implications. The opinions voiced are our own. We would like to tender apologies over those contributions we have not been able to include, but this is not, strictly speaking, a meeting review. Rather we have focused on those topics that bear more directly on evolutionary mechanisms, and have therefore slighted some presentations (including some of our own), that were oriented mainly toward developmental processes.

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GAL4 enhancer traps expressed in the embryo, larval brain, imaginal discs, and ovary of drosophila

et al. 1997

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We have screened a collection of approximately 400 GAL4 enhancer trap lines for useful patterns of expression in the embryo, larval brain, imaginal discs, and ovary using a UAS‐lacZ reporter construct. Although similar patterns of expression have previously been reported in the original P[lacZ] enhancer trap screens, these lines are useful for directing ectopic expression of genes in discrete patterns during these stages. In addition, we have identified some unique patterns of expression that have not been previously reported. Dev. Dyn. 209:310–322, 1997. © 1997 Wiley‐Liss, Inc.

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Integration of positional signals and regulation of wing formation and identity by Drosophila vestigial gene

Cited by 458 publications

References 48 publications

The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

The Drosophila wing differentiation factor Vestigial–Scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc

Themes from a NASA workshop on gene regulatory processes in development and evolution

GAL4 enhancer traps expressed in the embryo, larval brain, imaginal discs, and ovary of drosophila

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