Integration of Metabolic Reactions and Gene Regulation

Yeang, Chen‐Hsiang

doi:10.1007/s12033-010-9325-y

Cited by 10 publications

(4 citation statements)

References 127 publications

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“…The different forms of integration between metabolic pathways, cells, tissues, systems, and biomarkers have intrigued researchers over time, still representing a current and innovative scientific field [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Interleukin 12 correlates with performance, metabolism and acid-base balance during physical exercise

Castilho

Gonçalves

Rodolpho

et al. 2023

Preprint

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Studies involving physical exercise are no longer performed only to evaluate the performance of athletes, but have become an important tool to understand how different forms of stress affect immunometabolism. The present study investigated the acute impact of a treadmill running test on different biomarkers, the acid-base system, glycemia/lactatemia, and the correlation between IL-12 and metabolism/performance. Ten male subjects participated in a cross-sectional study. The treadmill protocol was progressively increased until exhaustion. The IL-12 concentration was measured using the "Cytometric Bead Array" kit (CBA, BD Bioscience, USA) through flow cytometry, and the data were analyzed using FCAP Array software. The test had an average time of 13 minutes and 51 seconds and induced alterations in IL-12 concentration of 160%, lactate of 607%, blood glucose of 58%, blood pH of -3%, BE of -529%, bicarbonate of - 58%, and anion gap of 232%. It was observed that the lower the percentage variation in IL-12, the greater the phase to reach the anaerobic threshold (AT) in Km/h, and the time to reach this same threshold, and the opposite was also true, confirmed by the Spearman test. (-0.900 between IL-12 and the time to reach AT and -0.872 between IL-12 and the phase to reach AT). Other correlations were observed: between post-IL-12 and pre anion gap of 1.0, post-IL-12 and post chloride of 1.0, percentage change in IL-12 and post anion gap of 1.0 and percentage variation in IL-12 and post lactate of 0.943, pre-IL-12 and post anion gap of -1.0, post-IL-12 and pre LDH of -0.943, post-IL-12 and post LDH of -0.943, post-IL -12 and BE post of -9.943, post-IL-12 and post bicarbonate of -0.943, and post-IL-12 and post pH of -0.943. The AT was reached in 7:52 minutes, in the 14.9 km/h phase, with a heart rate of 163 beats per minute, an absolute power of 524 W, and an absolute VO2 of 3.12 l.min. A correlation between IL-12 and performance, metabolism, and blood acid-base balance is suggested. Furthermore, it is expected that approximately 15% of glycemia is formed by the CORI cycle, through the removal of lactate and reestablishment of glycemia, however, this estimate can be exceeded in athletes, according to the level of training.

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Section: Introductionmentioning

confidence: 99%

Interleukin 12 correlates with performance, metabolism and acid-base balance during physical exercise

Castilho

Gonçalves

Rodolpho

et al. 2023

Preprint

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“…The comprehension of lag phase plays an important role in many aspects of the biotechnological field. As known, once bacteria are transferred to a new medium, the metabolite energy will be depleted in the cells and cause termination of the cell growth [1,2]. This phenomenon is explained by the differences between original media and the new culture.…”

Section: Introductionmentioning

confidence: 99%

Potential Cultivation of Lactobacillus pentosus from Human Breastmilk with Rapid Monitoring through the Spectrophotometer Method

et al. 2020

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The present study focused on the development of a new method to determine the lag phase of Lactobacillus in breast milk which was attained during the 1st, 3rd, and 6th month (M1, M3, and M6). The colonies’ phylogenetic analysis, derived from the 16S rRNA gene sequences, was evaluated with genus Lactobacillus pentosus and achieved a similarity value of 99%. Raman spectroscopy in optical densities of 600 nm (OD600) were used for six consecutive days to observe the changes of the cell growth rate. The values of OD600 were well fitted with the regression model. From this work, M1 was found to be the longest lag phase in 18 h, and it was 17% to 27% longer compared to M3 and M6, respectively. However, the samples of M3 and M6 showed the shortest duration in reaching 0.5 of OD600 nm (16 h) which was enhanced by 80% and 96% compared to M1, respectively. These studies will be of significance when applied in determining the bacteria growth curve and in assessing the growth behavior for the strain in human breast milk.

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“…In particular, samples come from two groups: cancer and benign. The intergration of such molecular data sources for exploration and discovery is of growing interest [5, 6, 7]. A variety of visualization tools have recently been introduced that can map metabolite and gene expression information onto metabolic pathways, such as [8, 9, 10].…”

Section: Introductionmentioning

confidence: 99%

Pathway-directed weighted testing procedures for the integrative analysis of gene expression and metabolomic data

et al. 2012

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We explore the utility of p-value weighting for enhancing the power to detect differential metabolites in a two-sample setting. Related gene expression information is used to assign an a priori importance level to each metabolite being tested. We map the gene expression to a metabolite through pathways and then gene expression information is summarized per-pathway using gene set enrichment tests. Through simulation we explore four styles of enrichment tests and four weight functions to convert the gene information into a meaningful p-value weight. We implement the p-value weighting on a prostate cancer metabolomics dataset. Gene expression on matched samples is used to construct the weights. Under certain regulatory conditions, the use of weighted p-values does not in-flate the type I error above what we see for the un-weighted tests except in high correlation situations. The power to detect differential metabolites is notably increased in situations with disjoint pathways and shows moderate improvement, relative to the proportion of enriched pathways, when pathway membership overlaps.

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Integration of Metabolic Reactions and Gene Regulation

Cited by 10 publications

References 127 publications

Interleukin 12 correlates with performance, metabolism and acid-base balance during physical exercise

Interleukin 12 correlates with performance, metabolism and acid-base balance during physical exercise

Potential Cultivation of Lactobacillus pentosus from Human Breastmilk with Rapid Monitoring through the Spectrophotometer Method

Pathway-directed weighted testing procedures for the integrative analysis of gene expression and metabolomic data

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