Integration of mechanical conditioning into a high throughput contractility assay for cardiac safety assessment

Goßmann, Matthias; Linder, Peter; Thomas, Ulrike; Juhász, Krisztina; Lemme, Marta; George, Michael; Fertig, Niels; Dragicevic, Elena; Stoelzle-Feix, Sonja

doi:10.1016/j.vascn.2020.106892

Cited by 7 publications

(1 citation statement)

References 63 publications

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“…Other technologies such as fibronectin lattice mesh biosensors 23 as well as Polydimethylsiloxane (PDMS) pillar integrated magnetic sensors 24 could monitor contractile strain or force of cardiac tissue but require optical imaging to derive such information. Commercial instruments such as FLEXcyte 96 25 , 26 system could measure the cardiomyocyte contractility through no-contact displacement induced capacitive changes but not for cardiac organoids.…”

Section: Introductionmentioning

confidence: 99%

A soft and ultrasensitive force sensing diaphragm for probing cardiac organoids instantaneously and wirelessly

et al. 2022

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Time-lapse mechanical properties of stem cell derived cardiac organoids are important biological cues for understanding contraction dynamics of human heart tissues, cardiovascular functions and diseases. However, it remains difficult to directly, instantaneously and accurately characterize such mechanical properties in real-time and in situ because cardiac organoids are topologically complex, three-dimensional soft tissues suspended in biological media, which creates a mismatch in mechanics and topology with state-of-the-art force sensors that are typically rigid, planar and bulky. Here, we present a soft resistive force-sensing diaphragm based on ultrasensitive resistive nanocracked platinum film, which can be integrated into an all-soft culture well via an oxygen plasma-enabled bonding process. We show that a reliable organoid-diaphragm contact can be established by an ‘Atomic Force Microscope-like’ engaging process. This allows for instantaneous detection of the organoids’ minute contractile forces and beating patterns during electrical stimulation, resuscitation, drug dosing, tissue culture, and disease modelling.

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