Multifactorial approaches to enhance maturation of human iPSC-derived cardiomyocytes

Kistamás, Kornél; Müller, Anna; Muenthaisong, Suchitra; Lamberto, Federica; Zana, Melinda; Dulac, Morgane; Leal, Filipa; Maziz, Ali; Costa, Pedro F.; Bernotienė, Eiva; Bergaud, Christian; Dinnyés, András

doi:10.1016/j.molliq.2023.122668

Cited by 2 publications

(2 citation statements)

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“…Electrically conductive materials hold significance in regenerative medicine, especially within cardiac tissue engineering, where scaffolds require biocompatibility, electrical conductivity, mechanical stability, and architectural resemblance to the native myocardium. [ 12,135,136 ] Effective propagation of electrical impulses within scaffolds is crucial to achieving synchronized contraction, a key aspect of cardiac function. CPs, in particular, have emerged as a promising tissue engineering tool due to their biocompatibility, electrical and ionic properties, flexibility, and versatility.…”

Section: Engineering Adult‐like Cms Using Cpsmentioning

confidence: 99%

“…Most hiPSC‐CMs differentiation protocols use small molecules on 2D chemically coated surfaces, with a notable but limited adoption of commercially available hiPSC‐CMs (probably due to their higher cost and chemically undefined nature). [ 12 ] Key considerations of the cryopreservation of hiPSC‐CMs include the optimal differentiation day for freezing, the use of pro‐survival treatments, and factors such as cell density, solution volume, freezing rates, and storage conditions. [ 13 ] Despite variability in methodologies, cryopreservation has been shown to yield high viability, recovery, and purity (>90%), [ 14 ] which is crucial for the hiPSC‐CMs' long‐term storage and supports biobanking initiatives.…”

Section: Introductionmentioning

confidence: 99%

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Advancing Cardiomyocyte Maturation: Current Strategies and Promising Conductive Polymer‐Based Approaches

Elkhoury,

Kodeih,

Enciso‐Martínez

et al. 2024

Adv Healthcare Materials

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Cardiovascular diseases are a leading cause of mortality and pose a significant burden on healthcare systems worldwide. Despite remarkable progress in medical research, the development of effective cardiovascular drugs has been hindered by high failure rates and escalating costs. One contributing factor is the limited availability of mature cardiomyocytes (CMs) for accurate disease modeling and drug screening. Human induced pluripotent stem cell‐derived CMs offer a promising source of CMs; however, their immature phenotype presents challenges in translational applications. This review focuses on the road to achieving mature CMs by summarizing the major differences between immature and mature CMs, discussing the importance of adult‐like CMs for drug discovery, highlighting the limitations of current strategies, and exploring potential solutions using electro‐mechano active polymer‐based scaffolds based on conductive polymers. However, critical considerations such as the trade‐off between three‐dimensional systems and nutrient exchange, biocompatibility, degradation, cell adhesion, longevity, and integration into wider systems must be carefully evaluated. Continued advancements in these areas will contribute to a better understanding of cardiac diseases, improved drug discovery, and the development of personalized treatment strategies for patients with cardiovascular disorders.This article is protected by copyright. All rights reserved

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Section: Engineering Adult‐like Cms Using Cpsmentioning

confidence: 99%