Integration of isothermal amplification with quantum dot-based fluorescence resonance energy transfer for simultaneous detection of multiple microRNAs

Abstract

The integration of quantum dot-based fluorescence resonance energy transfer with rolling circle amplification enables simultaneous sensitive detection of multiple microRNAs.

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

“…43,56,58 Several groups have reported the use of this protocol to conjugate QDs to peptides, 31,59 proteins, 60 antibodies 61,62 and nucleotides. 63,64 The method of streptavidin-biotin bonding was applied by Ghimire et al 65 to develop transparent thin films involving QDs conjugated to polymers. Initially, the chitosan-polystyrene (CS-PS) copolymer was reacted with biotin and then added to streptavidin-conjugated CdSe/ZnS QDs.…”

Accurate cancer cell identification and microRNA silencing induced therapy using tailored DNA tetrahedron nanostructures

“…With the development of nanomaterials, a variety of nanoprobes have been reported for the detection of multiple miRNAs. [9][10][11][12] For example, the Tang group developed multicolor uorescent nanoprobes based on gold nanoparticles (GNPs) for evaluating cellular migration and invasion by simultaneously imaging miRNA-221, PTEN mRNA and MMP9 in living cells. 13 The Zhu group assembled multicomponent nucleic acid enzymes onto the surface of mesoporous silica-coated gold nanorods as multifunctional nanodevices for intracellular miRNA-21 and miRNA-145 in situ imaging.…”

Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels

“… 9 – 12 Developing strategies for accurate and sensitive multiplex miRNA detection in living cells is significant for miRNA biomedical applications. 13 , 14 To address this issue, advanced delivery systems assisting the transfection of DNA probes into cells 15 , 16 and efficient intracellular signal amplification strategies for improving the detection sensitivity are urgently needed.…”