Integration of cytological and molecular analysis to confirm a hybridity in F1 Brassica progeny

Abstract

In Brassica, interspecific hybridization is a potential and useful method for transferring valuable traits between species of commercial interest. In the previous experiments successful interspecific hybrids were obtained through hybridization between chosen rapeseed cultivars (Brassica napus L.) and Brassica rapa genotypes for transferring clubroot resistance from wild species into cultivated background. In the presented research morphological, cytological and simple sequence repeats (SSR)based molecular analyses were carried out to confirm the hybrid nature of the F1 plants. This paper evidences for a successful creation of twenty three F1 interspecific hybrids where six out of eleven cross combinations were confirmed as fertile. The obtained results have shown that analyzed morphological characters of F1 hybrids were intermediate as compared to those of both parental genotypes. Furthermore, in pollen mother cells (PMCs) of the F1 hybrids abnormalities like univalent, lagging chromosomes and disorders in synchronization were observed in meiosis. Generally, the abnormalities in meiosis occurred in most of the tested genotypes with the mean frequency of 63.8%. Among tested SSR primers, Na10-A08 was found to reveal highly polymorphic bands in hybrids. As a result, 41.1% of the investigated plants were confirmed as true hybrids.

Tissue Culture Techniques for the Production of Interspecific Hybrids in Poland: History and Achievements

“…chinensis was used as the female parent and Brassica napus as the male parent (Niemann et al, 2015). True F 1 hybrids between these species were intermediate in terms of morphological traits compared with the parental genotypes; however, in their pollen mother cells (PMCs), various abnormalities, such as univalent, lagging chromosomes and disorders in synchronization, were observed during meiosis (Niemann et al, 2019).…”