Integration of a surface acoustic wave biosensor in a microfluidic polymer chip

Länge, Kerstin; Blaess, Guido; Voigt, A.; Götzen, Reiner; Rapp, M.

doi:10.1016/j.bios.2005.12.026

Cited by 75 publications

(42 citation statements)

References 12 publications

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“…Limits of detection of such devices are on the order of ng/cm 2 and recent lithographically fabricated devices (Rabe et al 2003;Zhang and Kim 2005) have demonstrated on-chip multiplexing with balance sizes on the order of a few 10 s of micrometers in radius. A variant on these BAW techniques are Surface Acoustic Wave, SAW, devices which exploit shear surface waves (Josse et al 2001;Lange et al 2006). Sensitivities are typically of the same order (Kalantar-Zadeh et al 2003) as QCM type devices.…”

Section: Acoustic Biosensorsmentioning

confidence: 99%

Nanobiosensors: optofluidic, electrical and mechanical approaches to biomolecular detection at the nanoscale

Erickson

Mandal

Yang

et al. 2007

Microfluid Nanofluid

218

142

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Next generation biosensor platforms will require significant improvements in sensitivity, specificity and parallelity in order to meet the future needs of a variety of fields ranging from in vitro medical diagnostics, pharmaceutical discovery and pathogen detection. Nano-biosensors, which exploit some fundamental nanoscopic effect in order to detect a specific biomolecular interaction, have now been developed to a point where it is possible to determine in what cases their inherent advantages over traditional techniques (such as nucleic acid microarrays) more than offset the added complexity and cost involved constructing and assembling the devices. In this paper we will review the state of the art in nanoscale biosensor technologies, focusing primarily on optofluidic type devices but also covering those which exploit fundamental mechanical and electrical transduction mechanisms. A detailed overview of next generation requirements is presented yielding a series of metrics (namely limit of detection, multiplexibility, measurement limitations, and ease of fabrication/assembly) against which the various technologies are evaluated. Concluding remarks regarding the likely technological impact of some of the promising technologies are also provided.

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Section: Acoustic Biosensorsmentioning

confidence: 99%

Nanobiosensors: optofluidic, electrical and mechanical approaches to biomolecular detection at the nanoscale

Erickson

Mandal

Yang

et al. 2007

Microfluid Nanofluid

218

142

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“…These devices usually consist of the sensor embedded in a shielding and protecting sensor cartridge or housing, typically manufactured from polymers. We and other groups have reported about the design of such sensor chips for SAW devices (Cole et al 2005;Francis et al 2004;Gronewold et al 2005;Länge et al 2006) which have proven to be suitable as cheap and disposable sensor elements. Sensor housings allow miniaturization of fluidic channels delivering the sample to the sensor, therefore low sample consumption can be obtained.…”

Section: Introductionmentioning

confidence: 98%

Design and integration of a generic disposable array-compatible sensor housing into an integrated disposable indirect microfluidic flow injection analysis system

Rapp

Schickling

Prokop

et al. 2011

Biomed Microdevices

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We describe an integration strategy for arbitrary sensors intended to be used as biosensors in biomedical or bioanalytical applications. For such devices ease of handling (by a potential end user) as well as strict disposable usage are of importance. Firstly we describe a generic array compatible polymer sensor housing with an effective sample volume of 1.55 μl. This housing leaves the sensitive surface of the sensor accessible for the application of biosensing layers even after the embedding. In a second step we show how this sensor housing can be used in combination with a passive disposable microfluidic chip to set up arbitrary 8-fold sensor arrays and how such a system can be complemented with an indirect microfluidic flow injection analysis (FIA) system. This system is designed in a way that it strictly separates between disposable and reusable components- by introducing tetradecane as an intermediate liquid. This results in a sensor system compatible with the demands of most biomedical applications. Comparative measurements between a classical macroscopic FIA system and this integrated indirect microfluidic system are presented. We use a surface acoustic wave (SAW) sensor as an exemplary detector in this work.

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“…For economical and feasibility reasons, it is therefore of great interest to significantly reduced consumption of these materials when possible. Consequently, different sensing techniques have been combined with microfluidic systems; for example surface plasmon resonance (SPR) (Sjölander and Urbaniczky 1991;Wheeler et al 2004), fluorescence microscopy (Marie et al 2006;Satoh et al 2007), SPR-induced fluorescence (Wiltschi et al 2006), electrochemical impedimetric spectroscopy (EIS) (Zou et al 2007), other impedance-based techniques (Ateya et al 2005;Boehm et al 2007), resonant acoustic profiling (RAP) (or quartz crystal microbalance, QCM) (Godber et al 2007), and surface acoustic wave sensors (SAW) (Länge et al 2006;Mitsakakis et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A miniaturized flow reaction chamber for use in combination with QCM-D sensing

Ohlsson

Axelsson

Henry

et al. 2010

Microfluid Nanofluid

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A miniaturized flow chamber for quartz crystal microbalance with dissipation monitoring (QCM-D) has been developed. The main purpose was to reduce the total liquid sample consumption during an experiment, but also to gain advantages with respect to kinetics and mass transport by reducing the boundary diffusion layer. The bottom of the flow chamber is a QCM-D sensor surface, on which a polydimethylsiloxane spacer ring, fabricated onto a poly(methyl methacrylate) lid, is placed symmetrically around the QCM-D electrode (diameter *10 mm). The spacer ring defines the inner chamber height (typically 40-50 lm) and provides sealing. Through the lid, there are inlet and outlet channels. The typical chamber volume is in the range of 2.5-3.5 ll (with a 10 ll dead volume). In flow mode, we have operated the cell at flow rates of 6-50 ll/ min, i.e., volume turnovers of 2-17 per min. As a model system, to test the microcell, the formation of supported phospholipid bilayers on a SiO 2 surface was studied. For comparison, the same process was studied in a commercially available QCM-D equipment with significantly larger total volume (by a factor of 20). The decrease in effective sample consumption to produce a bilayer on the sensor surface in the chamber was approximately proportional to the decrease in chamber volume. Smaller volume also reduced the liquid exchange time. Potential improvements of the chamber include further optimization of the flow profile and, in addition, further miniaturization by decreasing the chamber height and the sensor radius.

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Integration of a surface acoustic wave biosensor in a microfluidic polymer chip

Cited by 75 publications

References 12 publications

Nanobiosensors: optofluidic, electrical and mechanical approaches to biomolecular detection at the nanoscale

Nanobiosensors: optofluidic, electrical and mechanical approaches to biomolecular detection at the nanoscale

Design and integration of a generic disposable array-compatible sensor housing into an integrated disposable indirect microfluidic flow injection analysis system

A miniaturized flow reaction chamber for use in combination with QCM-D sensing

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