Integration of a multi-enzyme system with a liquid membrane in Taylor flow regime for the production and in situ recovery of gluconic acid from cellulose

Ruales‐Salcedo, Angela V.; Higuita, Juan C.; Fontalvo, Javier

doi:10.1016/j.cep.2020.108140

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…Researchers have developed many alternative processes to minimize the production costs associated with raw materials and product separation. Ruales-Salcedo et al proposed a hybrid process consisting of a multi-enzyme production system and in situ GA recovery by a liquid membrane in Taylor flow, which increased the GA productivity and yield to 1.6- and 1.7-fold higher than those in the multi-enzyme system containing sodium acetate buffer, respectively ( Ruales-Salcedo et al, 2020 ). Yu et al immobilized three types of enzymes (cellulase, GOD and catalase) on a reversible soluble Eudragit L-100 and then applied the system to the catalytic synthesis of GA from corn straw, with the conversion rate of cellulose to GA reaching 61.41% ( Yu et al, 2021 ).…”

Section: Innovative Integrated Routes For the Production Of Gluconic ...mentioning

confidence: 99%

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Gluconic acid (GA) and its derivatives, as multifunctional biological chassis compounds, have been widely used in the food, medicine, textile, beverage and construction industries. For the past few decades, the favored production means of GA and its derivatives are microbial fermentation using various carbon sources containing glucose hydrolysates due to high-yield GA production and mature fermentation processes. Advancements in improving fermentation process are thriving which enable more efficient and economical industrial fermentation to produce GA and its derivatives, such as the replacement of carbon sources with agro-industrial byproducts and integrated routes involving genetically modified strains, cascade hydrolysis or micro- and nanofiltration in a membrane unit. These efforts pave the way for cheaper industrial fermentation process of GA and its derivatives, which would expand the application and widen the market of them. This review summarizes the recent advances, points out the existing challenges and provides an outlook on future development regarding the production of GA and its derivatives by microbial fermentation, aiming to promote the combination of innovative production of GA and its derivatives with industrial fermentation in practice.

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Section: Innovative Integrated Routes For the Production Of Gluconic ...mentioning

confidence: 99%

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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“…( 8). 8 For this case, the GOx-CAT system was modeled for batch operation and the main assumptions are as follows: (12)] are summarized in Table 1.…”

Section: Model Solution and Optimization Methodologymentioning

confidence: 99%

“…As an important step towards this goal, in previous work, we successfully demonstrated a multi-enzyme system (composed of cellulase, ⊎-glucosidase, GOx, and CAT) for gluconic acid production using cellulosic material as the feedstock. 12,13 Process schemes and operational conditions for the multi-enzyme system have been proposed with the intent of evaluating and identifying the most promising process designs for the best use of the enzyme activity, while recognizing its cost contribution to production. The potential process schemes involve one or two bioreactors operating in either batch or fed-batch mode.…”

Section: Introductionmentioning

confidence: 99%

Modeling and tuning of glucose oxidase‐catalase system incorporating dissolved oxygen concentration

Ruales‐Salcedo

Prado-Rúbio

Fontalvo

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND Gluconic acid production by a glucose oxidase (GOx) and catalase (CAT) system has been proposed. However, the bioprocess optimal design and operation are limited by the lack of kinetic models of GOx involving oxygen as substrate. Herein, the GOx‐CAT system is modeled considering a continuous oxygen supply at a bioreactor lab scale. Initially, experiments and modeling for parameter estimations of KLa and the individual enzymes were conducted. Then, experiments and modeling for the GOx‐CAT system were performed for final model tuning. Additionally, the model quality was evaluated, allowing for a deeper understanding of the system phenomenology. RESULTS From the oxygen transport model tuning, a highly accurate KLa estimation was obtained (R2>0.98 and confidence interval <2%). The highest oxygen transport rate was obtained for the combined system Buffer‐antifoam‐GOx‐CAT that was 2.5 times larger than that obtained for DI water. Kinetic models for the individual enzymes were very accurate and the parameters were fully identifiable. For the integrated system, the gluconic acid evolution was properly predicted and there was a loss of predictive power for the oxygen model. Parameter identifiability analysis showed that kcatCAT and KMHPCAT interpretability was compromised. However, the sensitivity analysis indicated that the model must not be simplified. CONCLUSION Herein its has been demonstrated that KLa is a complex parameter to be estimated for multi‐enzymatic systems due to its dependency on medium composition, particularly with GOx. Nonetheless, based on the acceptable model predictive power, the calculated parameters could be used for studies on the process design phase. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Cellulase: A Catalytic Powerhouse for Lignocellulosic Waste Valorisation

Singh

Chakravarty

Khade

et al. 2022

Clean Energy Production Technologies

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Integration of a multi-enzyme system with a liquid membrane in Taylor flow regime for the production and in situ recovery of gluconic acid from cellulose

Cited by 7 publications

References 33 publications

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Modeling and tuning of glucose oxidase‐catalase system incorporating dissolved oxygen concentration

Cellulase: A Catalytic Powerhouse for Lignocellulosic Waste Valorisation

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