Integrating untargeted metabonomics, partial least square regression analysis and MetPA to explore the targeted pathways involved into Huangqi Jiangzhong Tang against chronic atrophic gastritis rats

Cui, Jiajia; Hu, Yinghuan; Du, Guanhua; Qin, Xuemei

doi:10.1016/j.chemolab.2017.03.005

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…In the present study, SA in the CG group was significantly up-regulated compared with the HC group (Fig. 6C), which agreed with a report that SA was significantly increased in rats with CAG [30,31]. Nicotinic acid, also known as niacin, is one of the naturally occurring vitamins B3.…”

Section: Energy Metabolism-supporting

confidence: 93%

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Non-targeted metabolomics reveals diagnostic biomarker in the tongue coating of patients with chronic gastritis

Wang

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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Analysis of the properties of the tongue has been used in traditional Chinese medicine for disease diagnosis. Notably, tongue analysis, which is non-invasive and convenient compared with gastroscopy and pathological examination, can be used to assess chronic gastritis (CG). In order to find potential diagnostic biomarkers and study the metabolic mechanisms of the endogenous small molecules in the tongue coating related to CG, a non-targeted metabolomic analysis method was developed using ultra high performance liquid chromatography combined with quadrupole timeof-flight mass spectrometry (UHPLC-Q/TOF-MS). It was performed using two different columns in positive and negative ion scanning modes separately. The stability of the samples was evaluated and the age and gender factors of the subjects were excluded to ensure the reliability of the data in this study. Finally, under the four analysis models, 130, 229, 113 and 92 differential compounds were found using multivariate statistical methods respectively. 37 potential biomarkers were putatively identified after removing the duplicate compounds and five potential diagnostic biomarkers were putatively identified by receiver operating characteristic (ROC) curve analysis, including inosine, oleamide, adenosine, N-acetylglucosamine (GlcNAc) and xanthine. The main metabolic pathways associated with CG were purine metabolism, amino acid metabolism, sphingolipid metabolism and energy metabolism, which suggested that oxygen free radicals and energy metabolism were altered in patients with CG. These results provided a potential new basis for the quantitative diagnosis and pathogenesis of CG.

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Section: Energy Metabolism-supporting

confidence: 93%

“…Creatinine is the end product of creatine and creatine phosphate metabolism in the body which is an essential substrate for arginine and proline metabolism and muscle energy [32]. Creatinine levels in the tongue coating of CG patients were significantly down-regulated, which was also found in the CAG rats [31].…”

Section: Amino Acid and Other Metabolisms-mentioning

confidence: 98%

Non-targeted metabolomics reveals diagnostic biomarker in the tongue coating of patients with chronic gastritis

Wang

et al. 2019

Journal of Pharmaceutical and Biomedical Analysis

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“…In previous reports, methods applied to ensure quality control of HQJZ had apparent limitations in that they could determine only one or two components. Besides, the analysis was very difficult to perform in order to investigate the intrinsic quality of HQJZ, while also insufficient to reveal the complexity and synergic effect of this formula (Liu, Cui, Hu, Du, & Qin, ). In this study, analytes with special biological activities were selected to evaluate the quality of the formula.…”

Section: Resultsmentioning

confidence: 99%

“…Heatmap was further constructed to reveal the relationships among the samples (Figure 3 (2), calycosin-7-glucoside (3), liquiritigenin (4), ononin (5), astraisoflavan-7-O-β-D-glucoside (6), calycosin (7), cinnamic acid (8), formononetin (9), 10-dimethoxymedicarpin (10), 7,2 0 -dihydroxy-3 0 ,4 0 -dimethoxyisoflavan (11), glycyrrhetinic acid (12), gallic acid (13), 7,2 0dihydroxy-3 0 ,4 0 -dimethoxyisoflavane-7-O-glucoside (14), astragaloside IV (15), astragaloside III (16), astragaloside II (17) In previous reports, methods applied to ensure quality control of HQJZ had apparent limitations in that they could determine only one or two components. Besides, the analysis was very difficult to perform in order to investigate the intrinsic quality of HQJZ, while also insufficient to reveal the complexity and synergic effect of this formula (Liu, Cui, Hu, Du, & Qin, 2017). In this study, analytes with special biological activities were selected to evaluate the quality of the formula.…”

Section: Hqjzp Is a Traditional Chinese Patent Medicine Consisting Ofmentioning

confidence: 99%

Rapid quantitative analysis of 18 chemical constituents in HuangQi JianZhong Tang based on UHPLC–MS

Jia

Qin

2020

Biomedical Chromatography

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A rapid, highly sensitive, and specific analytical method using ultra‐high‐performance liquid chromatography‐triple quadruple MS was developed to quantitatively measure 18 chemical constituents in Huangqi Jianzhong Tang (HQJZ), a famous traditional Chinese medicine formula. Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with a gradient mobile phase (A: 0.1% aqueous formic acid and B: acetonitrile) at a flow rate of 0.25 mL/min. The chromatographic peaks of 18 components were identified by comparing with the reference compounds. Multiple reaction monitoring was employed for quantitative analysis of the components. Seven batches of HQJZ samples were analyzed with a good linear regression relationship (R2, .9978–.9993), precisions [relative standard deviation (RSD), 0.90%–3.60%], repeatability (RSD, 2.50%–4.00%), stability (RSD, 1.00%–4.00%), and recovery (96.10%–104.30%). Based on this established method, the present study offered a highly sensitive, specific, and rapid determination method for identification of 18 compounds, which greatly promoted the systemic quality control of HQJZ.

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“…Previous studies have found that the metabolic regulations were contributed to the protection of HQJZ against CAG, where HQJZ was administrated accompanying with the duplication process of CAG rat model. [ ] The results only based on single analytical technology has illustrated that pretreatment of HQJZ could protect metabolic perturbations induced by CAG partially via metabolism of carbohydrates, amino acids or lipids, which could not simultaneously cover the whole metabolome due to their disadvantage in sensitivity or dynamic range. Therefore, studies are necessary to apply a multiplatform approach to increase the metabolic coverage.…”

Section: Introductionmentioning

confidence: 99%

Urinary metabolomics research for Huangqi Jianzhong Tang against chronic atrophic gastritis rats based on 1H NMR and UPLC-Q/TOF MS

Jin

Qin

Zheng

2020

Journal of Pharmacy and Pharmacology

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Objectives As a traditional Chinese medicine (TCM), Huangqi Jianzhong Tang (HQJZ) has a good efficacy in treating chronic atrophic gastritis (CAG). Our objective was to determine its mechanism based on the urine comprehensive metabolome. Methods In the study, a metabolomic approach was applied to reveal the efficacy of HQJZ on the constructed CAG rats coupled with proton nuclear magnetic resonance (1H NMR) and ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC‐Q/TOF MS). Key findings The results showed the regulatory effect of HQJZ on urinary metabolism disorder in CAG rats was similar to the positive drug teprenone. Nineteen and 16 potential biomarkers related to CAG were detected by NMR and UPLC‐Q/TOF MS, respectively. Thirty‐two urine metabolites were significantly regulated by HQJZ treatment. Combined with MetPA and partial least square regression analysis (PLS‐RA), three metabolic pathways of valine, leucine and isoleucine, TCA cycle, and glycine, serine and threonine metabolism were the most relevant pathways for HQJZ treatment. Conclusions The main mechanism of HQJZ might be due to the balance of energy consumption, inflammatory inhibition, improvement of the immune system and oxidative stress on the constructed CAG rats. These findings provided comprehensive metabolic information of TCM by parallel measurements by LC‐MS and NMR.

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Integrating untargeted metabonomics, partial least square regression analysis and MetPA to explore the targeted pathways involved into Huangqi Jiangzhong Tang against chronic atrophic gastritis rats

Cited by 16 publications

References 26 publications

Non-targeted metabolomics reveals diagnostic biomarker in the tongue coating of patients with chronic gastritis

Non-targeted metabolomics reveals diagnostic biomarker in the tongue coating of patients with chronic gastritis

Rapid quantitative analysis of 18 chemical constituents in HuangQi JianZhong Tang based on UHPLC–MS

Urinary metabolomics research for Huangqi Jianzhong Tang against chronic atrophic gastritis rats based on 1H NMR and UPLC-Q/TOF MS

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