Urinary metabolomics research for Huangqi Jianzhong Tang against chronic atrophic gastritis rats based on 1H NMR and UPLC-Q/TOF MS

Abstract

Objectives As a traditional Chinese medicine (TCM), Huangqi Jianzhong Tang (HQJZ) has a good efficacy in treating chronic atrophic gastritis (CAG). Our objective was to determine its mechanism based on the urine comprehensive metabolome. Methods In the study, a metabolomic approach was applied to reveal the efficacy of HQJZ on the constructed CAG rats coupled with proton nuclear magnetic resonance (1H NMR) and ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC‐Q/TOF MS). Key findings The results showed the regulatory effect of HQJZ on urinary metabolism disorder in CAG rats was similar to the positive drug teprenone. Nineteen and 16 potential biomarkers related to CAG were detected by NMR and UPLC‐Q/TOF MS, respectively. Thirty‐two urine metabolites were significantly regulated by HQJZ treatment. Combined with MetPA and partial least square regression analysis (PLS‐RA), three metabolic pathways of valine, leucine and isoleucine, TCA cycle, and glycine, serine and threonine metabolism were the most relevant pathways for HQJZ treatment. Conclusions The main mechanism of HQJZ might be due to the balance of energy consumption, inflammatory inhibition, improvement of the immune system and oxidative stress on the constructed CAG rats. These findings provided comprehensive metabolic information of TCM by parallel measurements by LC‐MS and NMR.

Jianpiyiqi formula ameliorates chronic atrophic gastritis in rats by modulating the Wnt/β‑catenin signaling pathway

“…Folate, as a vitamin, is able to improve gastric mucosal damage and teprenone is a gastric mucosal protective agent (34,35). In the present study, the positive control drugs selected for the rat model of CAG were folate and teprenone, as these have a certain effect to improve gastric atrophy according to previous experimental results (23,25). Furthermore, the positive control drugs inhibited the expression levels of Wnt signal-related genes.…”

“…In recent years, significant progress has been made in the treatment of patients with CAG using TCM (20,21). Previous in vivo studies have indicated that TCM formulations are able to improve gastric mucosal atrophy, including Modified Sijunzi Decoction (22), Weiqi Decoction (23), Banxia Xiexin Decoction (24) and Huangqi Jianzhong Tang (25). Jianpiyiqi formula is a TCM prescription that may be used to effectively treat CAG in the clinic (7,8).…”

Urine metabolomics of rats with chronic atrophic gastritis

“…[10] found that both electroacupuncture and moxibustion could restore various caG-induced metabolic changes, including membrane metabolism, energy metabolism and neurotransmitter function. Liu Yuetao [11] based on 1 H-NMR technical analysis astragalus chienchung soup to the CAG rats serum endogenous metabolites regulation function disorder, through multivariate statistical analysis, to clarify its regulation on chronic atrophic gastritis targets, related indicators associated with efficacy is established, by partial least-squares regression analysis and Met PA screening and treatment effect is most related metabolic pathways, find the root of remembranous milk vetch chienchung soup can obviously inhibit the CAG lesions, can obviously regulate 3hydroxy butyric acid lactic acid acetate succinate metabolites such as disorder, the CAG treatment is the main metabolic pathways of arginine-proline metabolismGlycerol metabolism and glycine-serine-threonine metabolism pathways. Sun Yina [12] found 59 qualitative and quantitative metabolites, and then PCA OPLS-DA VIP value was used to find 8 potential differential metabolites related to dampis-heat syndrome of spleen and stomach.…”

Explore the effects of Shidan granules on chronic atrophic gastritis using LC–MS based plasma metabolomics study

“…Vitacoenzyme (VTE) tablets have been clinically used to treat CAG due to their good curative effects (Liu et al, 2018; Liu et al, 2020); thus, VTE was selected as the positive drug in this study. After 7 days of acclimatization, 50 rats were randomly divided into five groups (10 rats in each group): control group, model group, VTE‐positive group (0.3 g/kg), low‐dose SDG group (14.04 g crude herbs per kg), and high‐dose SDG group (28.08 g crude herbs per kg).…”

“…Recently, traditional Chinese medicine (TCM) prescriptions, which have remarkable therapeutic effects with fewer side effects, have been widely used to treat CAG in clinics, (Liu et al, 2020; Xu et al, 2020). Among these TCM prescriptions, Shidan granules (SDG) have drawn extensive attention because of their excellent curative effects on CAG.…”