Integrating carbon nanotubes and lipid bilayer for biosensing

Huang, Yongmei; Palkar., Preeti Vikas; Li, Lain‐Jong; Zhang, Hua; Chen, Peng

doi:10.1016/j.bios.2009.12.011

Cited by 48 publications

(33 citation statements)

References 17 publications

(15 reference statements)

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“…However, for more practical use and realization of the full potential of these systems, we must now integrate these elements into functional devices, solve the lipid bilayer stability issues caused by the structural fragility of SLB in harsh conditions (such as dehydration and high salt concentration) and address nonspecific binding, especially for biosensing and cell-interfacing applications. 88,89 Furthermore, incorporating functional proteins such as transmembrane proteins into a lipid layer in an in vivo-like configuration while sustaining their functionalities is highly difficult. Finally, current techniques for generating nanoscale cell membrane features are mostly based on the creation of single heterogeneous membrane structures.…”

Section: Discussionmentioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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Cell membranes contain a variety of lipids and functional proteins that offer active platforms that organize the membrane components into functional assemblies and perform biologically important reactions. The dynamic and complex nature of the membranes makes them attractive materials, but at the same time, researchers report substantial experimental uncertainty in controlling the membrane reactions and extracting valuable information. The fascinating new structures and properties of nanomaterials could be utilized in addressing aforementioned issues, and the design and synthesis of lipid-nanostructure hybrids could be beneficial to the research areas in lipid-membrane biotechnology and nanobiotechnology. These hybrid structures possess dimensions that are comparable to that of biological molecules and structures and physicochemical properties that arise from both lipids and nanomaterials. Therefore, lipid-nanostructure hybrids offer additional options for control of the synthesized structures, provide new insight in understanding nanostructures and biological systems and allow the mimicking of functional subcellular membrane components and monitoring of the membrane-associated reactions in a highly sensitive and controllable manner. In this review, we present recent advances in the synthesis of various lipid-nanostructure hybrids and the application of these structures in biotechnology and nanotechnology. We further describe the scientific and practical applications of lipid-nanostructure hybrids for detecting membrane-targeting molecules, interfacing nanostructures with live cells and creating membrane-mimicking platforms to investigate various intercellular processes.

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Section: Discussionmentioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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“…6,11 In recent times, carbon nanotubes (CNTs) have been widely studied in the biomedical field for drug delivery, biosensing, and molecular imaging. [12][13][14] Biomedical properties like pharmacokinetics, toxicity, and cytocompatibility of graphene and GO have not been systematically explored. Yang et al 15 have reported pharmacokinetics and biodistribution of graphene functionalized with polyethylene glycol (PEG) and examined its toxicity in mice.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Gurunathan¹,

Han²,

Dayem³

et al. 2012

IJN

726

511

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Background: Graphene holds great promise for potential use in next-generation electronic and photonic devices due to its unique high carrier mobility, good optical transparency, large surface area, and biocompatibility. The aim of this study was to investigate the antibacterial effects of graphene oxide (GO) and reduced graphene oxide (rGO) in Pseudomonas aeruginosa. In this work, we used a novel reducing agent, betamercaptoethanol (BME), for synthesis of graphene to avoid the use of toxic materials. To uncover the impacts of GO and rGO on human health, the antibacterial activity of two types of graphene-based material toward a bacterial model P. aeruginosa was studied and compared. Methods: The synthesized GO and rGO was characterized by ultraviolet-visible absorption spectroscopy, particle-size analyzer, X-ray diffraction, scanning electron microscopy and Raman spectroscopy. Further, to explain the antimicrobial activity of graphene oxide and reduced graphene oxide, we employed various assays, such as cell growth, cell viability, reactive oxygen species generation, and DNA fragmentation. Results: Ultraviolet-visible spectra of the samples confirmed the transition of GO into graphene. Dynamic light-scattering analyses showed the average size among the two types of graphene materials. X-ray diffraction data validated the structure of graphene sheets, and high-resolution scanning electron microscopy was employed to investigate the morphologies of prepared graphene. Raman spectroscopy data indicated the removal of oxygen-containing functional groups from the surface of GO and the formation of graphene. The exposure of cells to GO and rGO induced the production of superoxide radical anion and loss of cell viability. Results suggest that the antibacterial activities are contributed to by loss of cell viability, induced oxidative stress, and DNA fragmentation. Conclusion:The antibacterial activities of GO and rGO against P. aeruginosa were compared. The loss of P. aeruginosa viability increased in a dose-and time-dependent manner. Exposure to GO and rGO induced significant production of superoxide radical anion compared to control. GO and rGO showed dose-dependent antibacterial activity against P. aeruginosa cells through the generation of reactive oxygen species, leading to cell death, which was further confirmed through resulting nuclear fragmentation. The data presented here are novel in that they prove that GO and rGO are effective bactericidal agents against P. aeruginosa, which would be used as a future antibacterial agent.

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“…Most of these modified NTFETs involve non-suspended SWNTs, [24][25][26][27] or a network of SWNTs. 28 Herein, we propose a novel single cell in vivo FET biosensor based on individual suspended SWNTs in a device that can function both as a FET and a p-n junction diode. Advantages of suspended SWNTs include a greater availability of surface area, which increases the sensitivity 1 of the device, and the ability of the cell to incorporate the nanotube internally while preserving the electrical connectivity to the carbon nanotube.…”

confidence: 99%

Single cell in-vivo carbon nanotube device with multimodal sensing potential

et al. 2013

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Single walled carbon nanotube (SWNT) field effect transistors (NTFETs) are quickly becoming the foundation for bioelectronic sensors. We describe a multimodal NTFET device that could be used as a real time single cell biosensor with the potential for chemical, optical and electrical sensing capabilities. This device utilizes the natural movement of a cell through the trench of a NTFET to provide a working cell-SWNT interaction where the nanotube is suspended. The use of individual suspended SWNTs in lieu of non-suspended SWNTs in our device provides the basis for an in vivo NTFET multimodal single cell biosensor.

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Integrating carbon nanotubes and lipid bilayer for biosensing

Cited by 48 publications

References 17 publications

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Single cell in-vivo carbon nanotube device with multimodal sensing potential

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