Integrating Anti-Influenza Virus Activity and Chemical Pattern Recognition to Explore the Quality Evaluation Method of Lonicerae Japonicae Flos

Xie, Xueqing; Gu, Lifei; Xu, Wanyi; Yu, Xie‐an; Yin, Guo; Wang, Jue; Jin, Ya; Wang, Lijun; Wang, Bing; Wang, Tiejie

doi:10.3390/molecules27185789

Cited by 8 publications

(15 citation statements)

References 45 publications

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“…LJF extract has been shown to not only reduce levels of IL-6 and TNF-α in order to reduce the inflammatory response but also to combat viral importation and replication by attenuating SARS-CoV-2 M pro activity [ 26 ]. Furthermore, LJF is regarded as a core anti-influenza herb, and its key chemical components against the influenza virus have been identified as neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid [ 27 ]. The repair rate of the lung index and the improvement rate of IL-6 and IFN-γ were chosen as the evaluation indexes of drug efficacy.…”

Section: Discussionmentioning

confidence: 99%

“…Chlorogenic acid has a broad spectrum of antiviral activity and could inhibit RSV infection by modulating the expression of TLR3, TBK1, and IRF3 in the TLR3 signaling pathway and decreasing the level of IFN-β [ 11 ]. Secoxyloganin plays an important role in the antiviral activity of LJF and may be related to the synergistic effect of multiple compounds [ 27 ]. This was in agreement with our results.…”

Section: Discussionmentioning

confidence: 99%

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Analyzing the Material Basis of Anti-RSV Efficacy of Lonicerae japonicae Flos Based on the PK-PD Model

Liang,

Liu,

Wang

et al. 2023

Molecules

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Lonicerae japonicae Flos (LJF) possesses a good anti-respiratory syncytial virus (RSV) effect. However, the material basis of LJF in treating RSV is still unclear. In this study, a sensitive and accurate quantitative method based on UHPLC-QQQ MS was established and validated for the simultaneous determination of the 15 ingredients from LJF in RSV-infected mice plasma. Multiple reaction monitoring was performed for quantification of the standards and of the internal standard in plasma. All the calibration curves show good linear regression within the linear range (r2 > 0.9918). The method validation results, including specificity, linearity, accuracy, precision, extraction recovery, matrix effect, and stability of 15 ingredients, are all within the current acceptance criteria. This established method was successfully applied to the pharmacokinetic study of 15 compounds from LJF. Furthermore, the repair rate of lung index and the improvement rate of IFN-γ and IL-6 improved after administration of the LJF, indicating that LJF possessed a positive effect on the treatment of RSV infection. Finally, by combining Spearman and Grey relation analysis, isochlorogenic acid B, isochlorogenic acid C, secoxyloganin, chlorogenic acid, and loganic acid are speculated to be the main effective ingredients of LJF in treating RSV. This study lays the foundation for attempts to reveal the mechanisms of the anti-RSV effect of LJF.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analyzing the Material Basis of Anti-RSV Efficacy of Lonicerae japonicae Flos Based on the PK-PD Model

Liang,

Liu,

Wang

et al. 2023

Molecules

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“…Traditional Chinese herbs are gaining attention as promising complementary and alternative therapies to fight infections [ 27 ]. LJF has been used for many years in China for its efficacy in the treatment of bacterial and viral infections, as documented in Mingyi Bielu [ 8 , 9 , 28 ]. LJF was the most popular traditional Chinese medicine used in treatments of severe acute respiratory syndromes (SARS) and influenza A in 2003 and 2009 [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Research by Gao et al [ 30 ] showed that LJF extracts exerted protective effects against Hydroquinone-induced cytotoxicity in hepatic L02 cells. Xie et al [ 9 ] explored the quality evaluation method of LJF by combining anti-H1N1 activity with chemical pattern recognition, which is of great significance for ensuring the clinical effect of LJF antiviral. Zeng et al [ 6 ] reviewed several anti-RSV efficacies of LJF.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology combined with metabolomics to explore the mechanism for Lonicerae Japonicae flos against respiratory syncytial virus

Ding,

Li,

Zhang

et al. 2023

BMC Complement Med Ther

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Background Respiratory Syncytial Virus (RSV) stands out as a primary contributor to lower respiratory tract infections and hospitalizations, particularly in infants. Lonicerae japonicae flos (LJF), a traditional Chinese medicine renowned for its efficacy against various viral infections, including RSV, has been widely employed. Despite its common use, the precise therapeutic mechanism of LJF against RSV remains elusive. This study aimed to investigate the underlying mechanism of LJF against RSV through network pharmacology and metabolomics. Methods In this study, based on network pharmacology, potential targets related to LJF and RSV were obtained from PubChem and Swiss Target Prediction. The core targets and pathways were established and verified by enrichment analysis and molecular docking. The anti-RSV efficacy of LJF was determined by in vitro experiments. Additionally, metabolomics analysis was integrated, allowing for the identification of differential metabolites and their correlation with targets following LJF treatment in the context of RSV infection. Results A total of 23 active ingredients and 780 targets were obtained, of which 102 targets were associated with LJF anti-RSV. The construction of the corresponding Protein–Protein Interaction (PPI) network unveiled potential core targets, including STAT3, TNF, and AKT1. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that LJF's anti-RSV effects primarily involve key pathways such as the PI3K-Akt signaling pathway, EGFR tyrosine kinase inhibitor resistance, and FoxO signaling pathway. Molecular docking showed that ZINC03978781, 4,5'-Retro-.beta.,.beta.-Carotene -3,3'-dione, 4',5'-didehydro and 7-epi-Vogeloside had better binding ability. The cellular assay showed that the therapeutic index of LJF against RSV was 4.79. Furthermore, 18 metabolites were screened as potential biomarkers of LJF against RSV, and these metabolites were mainly involved in the pathways of purine metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, and other related pathways. Conclusions The intergration of network pharmacology and metabolomics can clarify the active targets and related pathways of LJF against RSV, which could provide a valuable reference for further research and clinical application of LJF.

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“…Medicinal components have also been characterized. In the flowers, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 4,5- O -caffeoylquinic acid, sweroside and secoxyloganin have been found [ 15 ]. In the leaves, luteolin, rutin, luteoloside, lonicerin, isochlorogenic acid C, isochlorogenic acid B, isochlorogenic acid A and chlorogenic acid have been found [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry

Wang

et al. 2023

Metabolites

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Lonicera japonica Thunb. (LJT) has been widely used as medicines or food additives in Asian countries for thousands of years. The flower buds are often medicinally used, and the other tissues are ignored. However, flowers, leaves and stems have also been reported to have antimicrobial, anti-inflammatory and antioxidant effects. In the current study, un-targeted metabolomics analysis was performed to investigate the metabolic difference among different tissues (flowers, flower buds, stems and leaves) of LJT based on liquid chromatography with mass spectrometry. A total of 171 metabolites were identified, including 28 flavonoids, 35 phenolic acids, 43 iridoids, 9 amino acids, 6 nucleotides, 16 fatty acids, 22 lipids and 12 others. Four new secondary metabolites were discovered. Some flavonoids and iridoids were not detected in leaves and stems. Principal component analysis showed significant differences among four different tissues. Some 27, 81, 113 differential metabolites were found between flowers/flower buds, leaves/flower buds, stems/flower buds, respectively. Primary metabolites showed a higher content in the flowers and flower buds. For the flavonoids, flavones were mainly accumulated in the leaves, flavonols were mainly accumulated in the flower buds, and acylated flavonol glucosides were mainly accumulated in the flowers. Most phenolic acids showed a higher content in the flowers or flower buds, while phenolic acid-glucosides showed significantly higher content in the flower buds. The most abundant iridoids in the LJT also showed a higher content in the flowers and flower buds. These results can provide new insights into the understanding of the metabolites changes in different tissues, and lay a theoretical foundation for the comprehensive utilization of LJT.

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Integrating Anti-Influenza Virus Activity and Chemical Pattern Recognition to Explore the Quality Evaluation Method of Lonicerae Japonicae Flos

Cited by 8 publications

References 45 publications

Analyzing the Material Basis of Anti-RSV Efficacy of Lonicerae japonicae Flos Based on the PK-PD Model

Analyzing the Material Basis of Anti-RSV Efficacy of Lonicerae japonicae Flos Based on the PK-PD Model

Network pharmacology combined with metabolomics to explore the mechanism for Lonicerae Japonicae flos against respiratory syncytial virus

Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry

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