Integrating an algal β-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast

Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt

“…A recent study found that integrating a H. pluvialis β-carotene hydroxylase gene into the predesigned carotenoid-biosynthesis pathway in Kluyveromyces marxianus indeed gave higher carotenoid production than using crtS and crtR from X. dendrorhous. However, due to the low copy number of gene cassettes integrated into the genome, its total carotenoid productivity was still quite low (about 162 μg/g DCW) as compared with previous studies (Chang et al 2014). …”

RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

“…Metabolic engineering of carotenoid biosynthesis has been achieved in several plants including major crops such as rice, canola seeds, and tomato fruits [52][53][54]. Engineering high levels of b-carotene in the endosperm of 'golden rice' is a major breakthrough [55], and has the potential to improve the b-carotene source from the food, and the vitamin A content for human, reducing the disease of vitamin A defect especially for people from poor areas.…”

Carotenoids of Microalgae Used in Food Industry and Medicine

“…To speed up and reduce the costs of carotenoid production, other organisms than microalgae but modified genetically with algal genes have been proposed. For instance, Chang et al [434] have used yeast as an astaxanthin production platform. To reach this goal, they cloned β-carotenoid hydroxylases from different algae.…”