Integrated use of LC/MS/MS and LC/Q-TOF/MS targeted metabolomics with automated label-free microscopy for quantification of purine metabolites in cultured mammalian cells

Nybo, S. Eric; Lamberts, Jennifer T.

doi:10.1007/s11302-018-9643-2

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…Furthermore, the LC-MS technique has been improved to separate and identify more lipid classes and is currently considered a prominent metabolomics technique for metabolite identification [52]. Faster and more reliable technology for liquid chromatography coupled with quadrupole time of flight mass spectrometry (LC-Q/TOF-MS) has been developed for the simultaneous analysis of a large number of blood metabolites in metabolomics studies [33].…”

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confidence: 99%

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Guo

Tao

et al. 2020

The Journal of Veterinary Medical Science

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High-producing dairy cows are easily affected by left displacement of the abomasum (LDA) within 4 weeks postpartum. Although LDA is highly associated with metabolic disturbances, the related information on comprehensive metabolic changes, with the exception of some blood biochemical parameters, remains limited. In this study, the changes in plasma metabolites and in the metabolic profile of postpartum dairy cows with LDA were investigated through liquid chromatography coupled with quadrupole time of flight mass spectrometry (LC-Q/ TOF-MS)-based metabolomics, and the metabolic networks related to LDA were constructed through metabolomics pathway analysis (MetPA). An obvious change in the metabolic profile was reflected by significant variations in 68 plasma metabolites in postpartum dairy cows with LDA, and these variations consequently altered 13 metabolic pathways (histidine metabolism, tyrosine metabolism, valine, leucine and isoleucine biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, arginine and proline metabolism, tryptophan metabolism, synthesis and degradation of ketone bodies, linoleic acid metabolism, arachidonic acid metabolism, citrate cycle, butanoate metabolism, vitamin B 6 metabolism and pyrimidine metabolism). This study shows that the more detailed information obtained by LC-Q/TOF-MS-based metabolomics and MetPA might contribute to a better understanding of the disordered metabolic networks in postpartum dairy cows with LDA. KEY WORDS: left displacement of the abomasum, liquid chromatography coupled with quadrupole time of flight mass spectrometry, metabolomics, pathway analysis, postpartum dairy cow Left displacement of the abomasum (LDA) is often observed in high-producing dairy cows mainly within 4 weeks postpartum [2, 12, 14]. Specifically, LDA occurs when the abomasum migrates from its normal position to the left lateral abdominal wall, and this migration can cause anorexia and colic in dairy cows and even death in some cases [45]. Dairy cows with LDA exhibit a lower health status, decreased fertility, less milk production and hence a greater culling rate [11, 26]. Many studies have focused on factors related to LDA, and the findings suggest that the species, breed, gender, age, production level, nutrition, metabolism and concurrent diseases likely play an important role in the pathogenesis of LDA [13]. Marked variations in some blood biochemical parameters, such as amyloid A, haptoglobin, non-esterified fatty acids (NEFAs), beta-hydroxy-butyrate (BHBA), Ca, P, Mg, Cl, urea, glucose, aspartate aminotransferase and glutamate dehydrogenase, indicate that the metabolic status of lactating dairy cows with abomasum displacement is strikingly altered [18, 32, 34]. Nevertheless, the available information on comprehensive metabolic changes remains limited, and this topic should be further explored using omics technologies. Metabolomics, as a branch of omics research, aims to identify and quantify small endogenous metabolites in biological samples using a high-throughput analytical p...

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confidence: 99%

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Guo

Tao

et al. 2020

The Journal of Veterinary Medical Science

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“…Purine metabolites are considered clinically relevant biomarkers for improving the progression of PD. 37 Uric acid acts as a powerful antioxidant and an effective free radical scavenger in the human body. Its reduction might promote the occurrence and development of PD.…”

Section: Discussionmentioning

confidence: 99%

Rhynchophylline alleviates neuroinflammation and regulates metabolic disorders in a mouse model of Parkinson's disease

et al. 2023

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“…In order to address the low fragmentation efficiency of in‐source CID, the high collision energy characteristic of MS ALL technique was applied to LC–Q‐TOF‐MS. LC–Q‐TOF‐MS is a hybrid MS technology consisted of a unit mass resolution quadrupole (Q1), a second quadrupole (Q2), and a TOF mass analyzer with high resolution [211–218]. In the acquisition procedure of MS ALL technique, all the precursor ions are allowed to go through Q1 freely and fragmented in Q2.…”

Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non-toxic, non-immunogenic, non-antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins, or nanoparticles has become a mature technology for the sake of improving their pharmacokinetic and pharmacological profiles, also referred to as PEGylation. By comparison, there are only a few PEGylated pharmaceuticals have been registered for further clinical trials and even less was approved for marketing. High failure rate of PEGylated pharmaceuticals in pre-clinical and clinical trials could be majorly attributed to their unclear pharmacokinetic behaviors. Therefore, the in vivo fate of the PEGylated pharmaceuticals for the various routes of administration needs to be thoroughly investigated An accurate in vivo pharmacological study thereof highly depends on the precise detection of polyethylene glycols as well as their fragments in biological matrixes. The goal of this review is to highlight the analytical methods that were developed and applied to evaluate the polyethylene glycols in pharmaceutical ingredients and excipients, which bring us closer to bridging

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Integrated use of LC/MS/MS and LC/Q-TOF/MS targeted metabolomics with automated label-free microscopy for quantification of purine metabolites in cultured mammalian cells

Cited by 10 publications

References 22 publications

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses

Rhynchophylline alleviates neuroinflammation and regulates metabolic disorders in a mouse model of Parkinson's disease

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

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