Integrated transcriptome and proteome analysis reveals complex regulatory mechanism of cotton in response to salt stress

Abstract

Background Soil salt stress seriously restricts the yield and quality of cotton worldwide. To investigate the molecular mechanism of cotton response to salt stress, a main cultivated variety Gossypium hirsutum L. acc. Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis. Results Through transcriptome analysis in cotton leaves under salt stress for 0 h (T0), 3 h (T3) and 12 h (T12), we identified 8 436, 11 628 and 6 311 differentially expressed genes (DEGs) in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. A total of 459 differentially expressed proteins (DEPs) were identified by proteomic analysis, of which 273, 99 and 260 DEPs were identified in T3 vs. T0, T12 vs. T0 and T12 vs. T3, respectively. Metabolic pathways, biosynthesis of secondary metabolites, photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs. Detail analysis of the DEGs or DEPs revealed that complex signaling pathways, such as abscisic acid (ABA) and jasmonic acid (JA) signaling, calcium signaling, mitogen-activated protein kinase (MAPK) signaling cascade, transcription factors, activation of antioxidant and ion transporters, were participated in regulating salt response in cotton. Conclusions Our research not only contributed to understand the mechanism of cotton response to salt stress, but also identified nine candidate genes, which might be useful for molecular breeding to improve salt-tolerance in cotton.

Transcriptome Analysis Reveals Molecular Mechanisms under Salt Stress in Leaves of Foxtail Millet (Setaria italica L.)

“…Among the hub genes in the salt-tolerant accession, the four genes expressed only in the salt-sensitive accession were associated with the catabolism and synthesis of sugars, suggesting that salt-tolerant foxtail millet accession can catabolize to produce more energy under salt stress ( Figure 5 b). The relationship between genes (101760718 and 101781167) related to triosephosphate isomerase and enolase 1 and salt stress has been reported by Chen et al [ 63 ] and Nam et al [ 64 ]. However, to our knowledge, the response of alpha-1,4 glucan phosphorylase L isozyme (101773221) and glucose-1-phosphate adenylyltransferase small subunit 1 (101755337) has not been reported and requires further study ( Table 2 ).…”