Genome-wide identification of the CER1 gene family in apple and response of MdCER1-1 to drought stress

Gao, Yanlong; Zhang, Zhongxing; Cheng, Jiao; Xian, Xulin; Li, Cailong; Wang, Yanxiu

doi:10.1007/s10142-022-00940-x

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…The higher MDA content in the VIGS-silenced cotton suggests that the ability to remove ROS may be impaired [ 12 ]. Similarly, previous studies reported that CER are related to drought resistance in plants by modulating the accumulation of ROS [ 29 ]. As a kind of osmotic substance, proline content is an important index reflecting the degree of damage to plant membrane lipid peroxidation by stress [ 97 , 98 ].…”

Section: Discussionmentioning

confidence: 52%

“…The CER gene family contributes to the production of cuticle wax and has a vital function in stress mitigation [ 12 ]. The CER gene family has been found across the genome in a range of plant species, including Castanea mollissima [ 32 ], apple [ 29 ] and passion fruit ( Passiflora edulis ) [ 31 ], and functionally described in (A) thaliana [ 43 ], Barley [ 44 ], Brachypodium distachyon [ 45 ], and (B) napus [ 46 ]. However, the CER genes in cotton ( G. hirsutum ) have not yet been studied.…”

Section: Discussionmentioning

confidence: 99%

“…The FPKM expression results revealed higher expression levels of GhCER genes in the leaves of plants subjected to drought-stress when compared to well-watered plants, which is consistent with the qRT-PCR results indicating increased GhCER gene expression in the leaves of the drought-stressed plant when compared to the non-stressed plant group. Likewise, Gao et al [ 29 ] found that there was higher MdCER1 -1 expression in drought-stressed leaves when compared to leaves from irrigated plants [ 29 ]. The study by Wu et al [ 21 ] also highlighted that SlCER1 -1 induces wax alkanes synthesis, which improves drought resistance and fruit storage.…”

Section: Discussionmentioning

confidence: 99%

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Decoding drought resilience: a comprehensive exploration of the cotton Eceriferum (CER) gene family and its role in stress adaptation

Hamid,

Ghorbanzadeh,

Jacob

et al. 2024

BMC Plant Biol

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Background The cuticular wax serves as a primary barrier that protects plants from environmental stresses. The Eceriferum (CER) gene family is associated with wax production and stress resistance. Results In a genome-wide identification study, a total of 52 members of the CER family were discovered in four Gossypium species: G. arboreum, G. barbadense, G. raimondii, and G. hirsutum. There were variations in the physicochemical characteristics of the Gossypium CER (GCER) proteins. Evolutionary analysis classified the identified GCERs into five groups, with purifying selection emerging as the primary evolutionary force. Gene structure analysis revealed that the number of conserved motifs ranged from 1 to 15, and the number of exons varied from 3 to 13. Closely related GCERs exhibited similar conserved motifs and gene structures. Analyses of chromosomal positions, selection pressure, and collinearity revealed numerous fragment duplications in the GCER genes. Additionally, nine putative ghr-miRNAs targeting seven G. hirsutum CER (GhCER) genes were identified. Among them, three miRNAs, including ghr-miR394, ghr-miR414d, and ghr-miR414f, targeted GhCER09A, representing the most targeted gene. The prediction of transcription factors (TFs) and the visualization of the regulatory TF network revealed interactions with GhCER genes involving ERF, MYB, Dof, bHLH, and bZIP. Analysis of cis-regulatory elements suggests potential associations between the CER gene family of cotton and responses to abiotic stress, light, and other biological processes. Enrichment analysis demonstrated a robust correlation between GhCER genes and pathways associated with cutin biosynthesis, fatty acid biosynthesis, wax production, and stress response. Localization analysis showed that most GCER proteins are localized in the plasma membrane. Transcriptome and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) expression assessments demonstrated that several GhCER genes, including GhCER15D, GhCER04A, GhCER06A, and GhCER12D, exhibited elevated expression levels in response to water deficiency stress compared to control conditions. The functional identification through virus-induced gene silencing (VIGS) highlighted the pivotal role of the GhCER04A gene in enhancing drought resistance by promoting increased tissue water retention. Conclusions This investigation not only provides valuable evidence but also offers novel insights that contribute to a deeper understanding of the roles of GhCER genes in cotton, their role in adaptation to drought and other abiotic stress and their potential applications for cotton improvement.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Decoding drought resilience: a comprehensive exploration of the cotton Eceriferum (CER) gene family and its role in stress adaptation

Hamid,

Ghorbanzadeh,

Jacob

et al. 2024

BMC Plant Biol

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“…This finding suggested that NRT1.1 may regulate NO 3 − -dependent Na + transport. Apples, as an important horticultural product, have significant economic benefits globally [39][40][41][42][43][44]. China has the highest apple production and nitrogen fertilizer application rates in the world.…”

Section: Introductionmentioning

confidence: 99%

“…China has the highest apple production and nitrogen fertilizer application rates in the world. Excess nitrogen fertilizer is applied to cultivated apple trees to achieve high production, but the absorption efficiency of the plant body is less than 40%, which is a large discrepancy compared to developed countries [40][41][42][43][44] and causes serious environmental problems. Furthermore, the relatively poor local climate of apple orchards in China (mainly in mountainous, rural areas), insufficient rainfall, and reduced groundwater level, as well as soil salinization caused by unreasonable irrigation and single application chemical fertilizer over a long time period, mean that the apple trees are to a large extent vulnerable to drought and salt stress, resulting in a decline in apple yield and quality.…”

Section: Introductionmentioning

confidence: 99%

Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica

Liu

Lin

Yang

et al. 2023

IJMS

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Nitrate is one of the main sources of nitrogen for plant growth. Nitrate transporters (NRTs) participate in nitrate uptake and transport, and they are involved in abiotic stress tolerance. Previous studies have shown that NRT1.1 has a dual role in nitrate uptake and utilization; however, little is known about the function of MdNRT1.1 in regulating apple growth and nitrate uptake. In this study, apple MdNRT1.1, a homolog of Arabidopsis NRT1.1, was cloned and functionally identified. Nitrate treatment induced an increased transcript level of MdNRT1.1, and overexpression of MdNRT1.1 promoted root development and nitrogen utilization. Ectopic expression of MdNRT1.1 in Arabidopsis repressed tolerance to drought, salt, and ABA stresses. Overall, this study identified a nitrate transporter, MdNRT1.1, in apples and revealed how MdNRT1.1 regulates nitrate utilization and abiotic stress tolerance.

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Identification and Expression Analysis of MdFAR Gene Family Based on Transcriptome and Physiological Data in Apple (Malus × domestica)

Ma,

Gao,

Zhang

et al. 2024

J Plant Growth Regul

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Genome-wide identification of the CER1 gene family in apple and response of MdCER1-1 to drought stress

Cited by 9 publications

References 37 publications

Decoding drought resilience: a comprehensive exploration of the cotton Eceriferum (CER) gene family and its role in stress adaptation

Decoding drought resilience: a comprehensive exploration of the cotton Eceriferum (CER) gene family and its role in stress adaptation

Identification and Functional Characterization of MdNRT1.1 in Nitrogen Utilization and Abiotic Stress Tolerance in Malus domestica

Identification and Expression Analysis of MdFAR Gene Family Based on Transcriptome and Physiological Data in Apple (Malus × domestica)

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