Integrated study of genotoxicity biomarkers in schoolchildren and inhalable particles in areas under petrochemical influence

Lemos, Andréia Torres de; Lemos, Clarice Torres de; Coronas, Mariana Vieira; Rocha, Jéssica Rosiak da; Vargas, Vera Maria Ferrão

doi:10.1016/j.envres.2020.109443

Cited by 15 publications

(7 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The comet test did not show any association with air pollutants, but the DNA damage appeared higher during the second winter in the presence of higher air pollution levels. Similar results have been reported by other authors, highlighting that MN and comet tests detect independent effects induced by different substances not necessarily attached to ultrafine particulate matter [23,36,55], in contrast to other studies showing a concordance among these tests, including if with lower concentration of air pollutants [49].…”

Section: Discussionsupporting

confidence: 90%

“…Conversely, this study design did not allow to compare the MN and comet test results at high and low air pollutants levels. Various studies found a positive association between air pollution and DNA damage tests at lower levels of air pollutants than that found in our study [40][41][42][43][44][45][46][47][48][49]. However, other studies provided negative or inconsistent results regarding the association between MN frequency in children's cells and air pollutant exposure, highlighting the role of other behavioural factors in the MN occurrence [47,52,53].…”

Section: Discussionsupporting

confidence: 47%

“…A previous study conducted on preschool children (3-6 years old) living in the same town [39] during two consecutive winters, also found a high mean MN frequency in children's buccal mucosa cells, similarly to that found in present study. Other studies evaluated the frequency of MN in exfoliated buccal cells in children [40][41][42][43][44][45][46][47][48][49] and found a significant association between MN frequencies and various air pollutants in children exposed to air pollution.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

et al. 2021

View full text Add to dashboard Cite

Air pollutants, especially PM, have been found to determine various effects on human health, including genotoxic effects. The aim of this study was to assess DNA damage with micronuclei (MN) and comet tests on buccal cells of 6–8 years old children living in an area with high air pollution. Both tests were repeated in the same children in two consecutive winters to compare the levels of DNA damage under different pollution conditions. A complete data set including lifestyle, air pollutants levels and biological sampling was available for 180 children in the two winters. A high mean MN frequency was found in both seasons, with higher value in the first (0.51 ± 0.59) than the second winter (0.40 ± 0.52), whereas DNA damage measured with comet test showed higher damage in the second versus the first winter (visual score 208.8 ± 67.1 vs. 173.2 ± 50.8). The associations between air pollutant levels (CO, NO2, SO2, benzene, O3, PM10, and PM2.5; PM0.5 and PAHs) and DNA damage were investigated at different lag times, and mainly, no significant association was found. This study on repeated measure of MN frequency and DNA damage in children’s buccal did not show an association with various air pollutants evaluated in an area with high levels of air pollution.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Characteristics of the included studies are reported in Table 1 and Table 2 and Supplementary Table S3 . Five studies were carried out in Europe [ 73 , 74 , 75 , 76 , 77 ] and South America [ 78 , 79 , 80 , 81 , 82 ], and three studies were conducted in Asia [ 83 , 84 , 85 ]. The first paper was published in 2000 [ 85 ], whereas the most recent one was released online in December 2020 [ 83 ].…”

Section: Resultsmentioning

confidence: 99%

Cytogenetic Effects in Children Exposed to Air Pollutants: A Systematic Review and Meta-Analysis

Acito

Fatigoni

Villarini

et al. 2022

IJERPH

View full text Add to dashboard Cite

The aim of this systematic review and meta-analysis was to assess the association between exposure to ambient air pollutants and micronuclei (MN) frequency in children. This work was performed according to the Cochrane Collaboration and the PRISMA guidelines and recommendations. Articles published before November 2021 were identified by an advanced search on PubMed/MEDLINE, Scopus and Web of Science databases. A critical appraisal using a specific tool was conducted to assess the quality of each included study. All analyses were carried out by using the Review Manager (RevMan) 5.4 software (The Cochrane Collaboration, London, UK). One hundred and forty-five references were firstly identified, and, at the end of selection process, 13 studies met the inclusion criteria. Six studies carried out a direct evaluation through the use of air samplers, whereas the other ones accessed environmental databases (n = 2) or used other tools (n = 3). In two cases, exposure was not directly investigated, with children sampled in two different areas with well-known different levels of pollution. The overall effect size (ES) was 1.57 ((95% CI = 1.39; 1.78), p-value < 0.00001) (total evaluated subjects: 4162), which highlighted a statistically significant association between outdoor air pollution and MN frequency in children. As a high MN frequency has been associated with a number of pathological states and a higher risk of developing chronic degenerative diseases, our results should be taken into consideration by policy makers to design and implement interventions aimed at reducing the introduction of pollutants in the atmosphere as well as at minimizing the exposure extent, particularly in children.

show abstract

“…The micronucleus test in human peripheral blood lymphocytes has been used since 1976 (Countryman and Heddle 1976), being the most frequently cytogenetic parameter in studies involving molecular epidemiology (Sram et al 2016). Nevertheless, the use of oral exfoliated cells is of great importance in detecting genotoxic effects since they have rapid renewal, are correlated with micronucleus marking in lymphocytes, and are the first contact of several carcinogenic agents that occur by inhalation and ingestion, establishing an in vivo examination in a target tissue: the oral epithelium (Lemos et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Is micronucleus assay in oral exfoliated cells a suitable tool for biomonitoring children exposed to environmental pollutants? A systematic review

Malacarne

Souza

Alpire

et al. 2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

The aim of this review was to evaluate if micronucleus assay in oral exfoliated cells is a suitable tool for biomonitoring children exposed to environmental pollutants. Through the electronic databases PubMed/Medline, Scopus, and Web of Science, all published studies until April 2021 that examined the relationship between exposure to environmental pollutants and micronucleus frequency in oral cells were searched. All relevant articles using a combination of the following keywords-"children," "micronucleus," "oral cells," and "environmental pollution"-were considered. A total of 20 papers met the criteria for inclusion in the systematic review. The results regarding the cytogenetic damage induced by environmental pollutants are conflicting. Some authors have demonstrated that environmental pollution induces mutagenesis in oral cells while others did not. Following the parameters of the Project for Effective Public Health Practices (EPHPP) and after extensive reading of all the articles included, a total of 12 articles had moderate and strong scores and 8 had a classification considered weak. Taken together, this review was able to demonstrate the association between micronucleus frequency and exposure to environmental pollutants in oral exfoliated cells of children.

show abstract

Integrated study of genotoxicity biomarkers in schoolchildren and inhalable particles in areas under petrochemical influence

Cited by 15 publications

References 65 publications

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

Winter Air Pollution and Genotoxic Effects in Children Living in a Highly Polluted Urban Area

Cytogenetic Effects in Children Exposed to Air Pollutants: A Systematic Review and Meta-Analysis

Is micronucleus assay in oral exfoliated cells a suitable tool for biomonitoring children exposed to environmental pollutants? A systematic review

Contact Info

Product

Resources

About