Integrated structural and functional optical imaging combining spectral-domain optical coherence and multiphoton microscopy

Vinegoni, Claudio; Ralston, Tyler S.; Tan, Wei; Luo, Wenhua; Marks, D.L.; Boppart, Stephen A.

doi:10.1063/1.2171477

Cited by 76 publications

(55 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fibroblast cell migration in response to injury was monitored by MPM using TPF. Vinegoni et al 188 reported an integrated microscope that combined SD OCM, TPF, and SHG signals to allow for simultaneous acquisition of structural and functional information based on a single Ti:sapphire laser with a 60-nm bandwidth and 80-MHz pulse repetition rate (Femtolasers, Vienna, Austria). An average sample power between 1 and 10 mW was focused with a 20 × 0.95 MO onto fibroblast cells transfected with GFP-labeled vinculin and stained with a nuclear dye (Hoechst 33342).…”

Section: Multimodal Oct and Mpmmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

418

264

View full text Add to dashboard Cite

Abstract. In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submicron regime and to the imaging speed increase by more than half a million times to more than 5 million A-scans per second, with the latter one accomplished by the state-of-the-art swept source laser technologies that are reviewed in this article. In addition, parallelization of OCT detection, such as line-field and full-field OCT, has shortened the acquisition time even further by establishing quasi-akinetic scanning. Besides the technical improvements, several functional and contrast-enhancing OCT applications have been investigated, among which the label-free angiography shows great potential for future studies. Finally, various multimodal imaging modalities with OCT incorporated are reviewed, in that these multimodal implementations can synergistically compensate for the fundamental limitations of OCT when it is used alone. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Multimodal Oct and Mpmmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

418

264

View full text Add to dashboard Cite

show abstract

“…Multimodal MPT/ OCM systems capable of obtaining MPT and OCM images simultaneously have been previously reported in cell cultures and tissue models, and have demonstrated that complimentary information can be obtained from biological tissues [22][23][24][25]. Significant improvements in terms of system design, light source, image resolution, acquisition speed and postprocessing algorithm have since been achieved [26,27].…”

Section: Biophotonicsmentioning

confidence: 99%

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Alex

Weingast

Weinigel

et al. 2012

Journal of Biophotonics

View full text Add to dashboard Cite

A preliminary clinical trial using state‐of‐the‐art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three‐dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub‐cellular details with axial and transverse resolutions of <2 μm and <0.5 μm, respectively, from a volume of 0.35 × 0.35 × 0.2 mm3 at a frame rate of 0.14 Hz (512 × 512 pixels). State‐of‐the‐art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ∼8 μm and ∼20 μm, respectively, from a volume of 7 × 3.5 × 1.5 mm3 at a frame rate of 46 Hz (1024 × 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre‐screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub‐cellular level information of the respective regions (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

“…One of the limits of 3D deconvolution is that it tends to reduce the sensitivity by the introduction of noise [11] and because of the required high phase stability it could not be applied to in vivo imaging [10]. SD-OCT has been applied to cellular imaging [2][3][4][5] and also offers the benefit of increased phase stability [12][13][14][15].…”

Section: Biophotonicsmentioning

confidence: 99%

“…Substrates involved are often opaque which causes difficulties for conventional cellular imaging modalities such as differential interference contrast (DIC) microscopy. Therefore optical coherence tomography (OCT) has been proposed to be used for time lapse cell imaging in 3D scaffolds [1][2][3][4][5]. Difficulties arise since OCT signals from cell samples exhibit high dynamic range.…”

Section: Introductionmentioning

confidence: 99%

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Höfer¹,

Povazˇay²,

Hermann³

et al. 2011

Journal of Biophotonics

View full text Add to dashboard Cite

Visualization of cell migration during chemotaxis using spectral domain optical coherence tomography (OCT) requires non‐standard processing techniques. Stripe artefacts and camera noise floor present in OCT data prevent detailed computer‐assisted reconstruction and quantification of cell locomotion. Furthermore, imaging artefacts lead to unreliable results in automated texture based cell analysis. Here we characterize three pronounced artefacts that become visible when imaging sample structures with high dynamic range, e.g. cultured cells: (i) time‐varying fixed‐pattern noise; (ii) stripe artefacts generated by background estimation using tomogram averaging; (iii) image modulations due to spectral shaping. We evaluate techniques to minimize the above mentioned artefacts using an 800 nm optical coherence microscope. Effect of artefact reduction is shown exemplarily on two cell cultures, i.e. Dictyostelium on nitrocellulose substrate, and retinal ganglion cells (RGC‐5) cultured on a glass coverslip. Retinal imaging also profits from the proposed processing techniques. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Integrated structural and functional optical imaging combining spectral-domain optical coherence and multiphoton microscopy

Cited by 76 publications

References 12 publications

Optical coherence tomography today: speed, contrast, and multimodality

Optical coherence tomography today: speed, contrast, and multimodality

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Contact Info

Product

Resources

About