Integrated Stirred-Tank Bioreactor with Internal Adsorption for the Removal of Ammonium to Enhance the Cultivation Performance of gdhA Derivative Pasteurella multocida B:2

Abstract

Growth of mutant gdhA Pasteurella multocida B:2 was inhibited by the accumulation of a by-product, namely ammonium in the culture medium during fermentation. The removal of this by-product during the cultivation of mutant gdhA P. multocida B:2 in a 2 L stirred-tank bioreactor integrated with an internal column using cation-exchange adsorption resin for the improvement of cell viability was studied. Different types of bioreactor system (dispersed and internal) with resins were successfully used for ammonium removal at different agitation speeds. The cultivation in a bioreactor integrated with an internal column demonstrated a significant improvement in growth performance of mutant gdhA P. multocida B:2 (1.05 × 1011 cfu/mL), which was 1.6-fold and 8.4-fold as compared to cultivation with dispersed resin (7.2 × 1010 cfu/mL) and cultivation without resin (1.25 × 1010 cfu/mL), respectively. The accumulation of ammonium in culture medium without resin (801 mg/L) was 1.24-fold and 1.37-fold higher than culture with dispersed resin (642.50 mg/L) and culture in the bioreactor integrated with internal adsorption (586.50 mg/L), respectively. Results from this study demonstrated that cultivation in a bioreactor integrated with the internal adsorption column in order to remove ammonium could reduce the inhibitory effect of this by-product and improve the growth performance of mutant gdhA P. multocida B:2.

Development of In Situ Product Recovery (ISPR) System Using Amberlite IRA67 for Enhanced Biosynthesis of Hyaluronic Acid by Streptococcus zooepidemicus

“…The increased yield may be due to suitable environmental conditions created by this method. The surface of the resin adsorbent packed in the column can be increased because the use of an internal column allows the fluidization of porous adsorbent resin; therefore, more adsorbates can be adsorbed [ 44 ]. To date, columnar resins have been used to enhance multiple product biosynthesis, overcome the product inhibitory effect, and streamline the procedure for product recovery.…”

“…The concave surface resins (for the resins in the dispersed system) may be caused by the direct shear force from the impeller and increasing rate of resin collision, which affects the stability and efficacy of the resins. The internal column system allows the resins to adsorb the target product by being entrapped inside the column with no direct contact with the impeller [ 44 ]. Furthermore, the life cycle for IRA67 is higher in the internal column than in the dispersed system, as the letter, the concave-shaped surface, may clog the resin pores and cause fouling of the resin [ 61 ].…”

Microbial Secondary Metabolism and Biotechnology

“…Oslan et al (2020) [23] worked on improving the growth and viability of the mutant gdhA Pasteurella multocida B:2. As they have demonstrated that gdhA P. multocida B:2 growth was inhibited by the accumulation of by-product ammonium in the culture medium, they developed a 2 L stirred-tank bioreactor integrated with an internal column using cationexchange adsorption resin.…”

Innocell Bioreactor: An Open-Source Development to Produce Biomaterials for Food and Packaging Based on Fermentation Processes

“…They consist of a tank equipped with an agitator for mixing and aeration. Stirred tank bioreactors allow for controlled fermentation conditions such as temperature, pH, and dissolved oxygen levels [39,40].…”