Development of In Situ Product Recovery (ISPR) System Using Amberlite IRA67 for Enhanced Biosynthesis of Hyaluronic Acid by Streptococcus zooepidemicus

Abstract

High broth viscosity due to the accumulation of hyaluronic acid (HA) causes a limited yield of HA. It is a major problem of HA production using Streptococcus zooepidemicus. Extractive fermentation via in situ product recovery (ISPR) was utilized to enhance the HA production. Resins from Amberlite: IRA400 Cl; IRA900 Cl; IRA410 Cl; IRA402 Cl; and IRA67 were tested for the HA adsorption. IRA67 showed high adsorption capacity on HA. The study of the adsorption via a 2 L stirred tank bioreactor of S. zooepidemicus fermentation was investigated to elucidate the adsorption of HA onto IRA67 in dispersed and integrated internal column systems. The application of a dispersed IRA67 improved the HA production compared to the fermentation without resin addition by 1.37-fold. The HA production was further improved by 1.36-fold with an internal column (3.928 g/L) over that obtained with dispersed IRA67. The cultivation with an internal column shows the highest reduction of viscosity value after the addition of IRA67 resin: from 58.8 to 23.7 (mPa·s), suggesting the most effective ISPR of HA. The improved biosynthesis of HA indicated that an extractive fermentation by ISPR adsorption is effective and may streamline the HA purification.

The synergetic effect from the combination of different adsorption resins in batch and semi‐continuous cultivations of S. Cerevisiae cell factories to produce acetylated Taxanes precursors of the anticancer drug Taxol

“…In addition, solid extractants for ISPR have also been widely used, where common materials used to achieve the recovery of products involve: activated charcoal, silica gel and adsorbent porous resins among others (Abdullah Thaidi et al, 2023;Lee et al, 2003;Othman et al, 2018).…”

“…In addition, solid extractants for ISPR have also been widely used, where common materials used to achieve the recovery of products involve: activated charcoal, silica gel and adsorbent porous resins among others (Abdullah Thaidi et al, 2023 ; Lee et al, 2003 ; Othman et al, 2018 ). An important advantage of using a solid extractant is the easiness to be separated from the media by centrifugation or filtration steps as well as the easy recovery of the desired product from the solid phase (Aguilar et al, 2019 ).…”

Microbial hyaluronic acid production in the 21 century: a roadmap toward high production, tailored molecular weight

“…60.5 mPa s), improving oxygen transfer. No information was found about effects on molecular weight (ABDULLAH THAIDI et al, 2023).…”

Applications of Hyaluronic Acid (HA) in Dental Implant Treatment: A Systematic Review

“…It is present naturally in animals and humans and has the fantastic ability to hold up to 1000 times the weight of water (7). Native HA is present in the human body as a significant component in the extracellular matrix (ECM) and can be found in many tissues (6,8). HA functions in moisturizing the tissues and keeping the viscoelastic form of ECM (9).…”

“…Bioengineered synthesis of HA from an enzymatic process is a much simpler procedure and reduces the risk of contamination. For example, HA can be biosynthesized from Streptococcus zooepidemicus bacteria (8). However, some modifications need to be done for the extracted HA to make it compatible with human tissues (6).…”