Integrated regulation triggered by a cryophyte ω-3 desaturase gene confers multiple-stress tolerance in tobacco

Shi, Yulan; Yue, Xiule; An, Lizhe

doi:10.1093/jxb/ery050

Cited by 41 publications

(29 citation statements)

References 107 publications

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“…FTM1 participates alongside FAD3 in the mediation of stresses associated with Arabidopsis crown gall development through their enrichment with C18:3 fatty acids (Klinkenberg et al, 2014). The increase of C18:3 class of fatty acids also correlated with the enhanced membrane stability measures in the CbFAD3-OE lines (Shi et al, 2018). These results therefore suggest a role for SKU5 in stress metabolism with respect to the plasma membrane, a role that was not revealed in previous ground experiments.…”

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 57%

“…SKU5 may also directly participate in stress response signaling or cellular remodeling due to its localization to the outer aspects of the plasma membrane. A SKU5 homolog is highly upregulated [Log 2 (14)] in tobacco overexpressing a FATTY ACID DESATURASE 3 (CbFAD3-OE) homolog derived from the cryophyte Chorispora bungeana when compared to a wildtype line in the response to salt stress (Shi et al, 2018). CbFAD3-OE lines exhibited enhanced membrane fluidity and survival under cold, salt, and drought stresses, and demonstrated reduced lipid peroxidation and membrane leakage via activation of calcium signaling and suppression of ROS (Shi et al, 2018).…”

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

“…A SKU5 homolog is highly upregulated [Log 2 (14)] in tobacco overexpressing a FATTY ACID DESATURASE 3 (CbFAD3-OE) homolog derived from the cryophyte Chorispora bungeana when compared to a wildtype line in the response to salt stress (Shi et al, 2018). CbFAD3-OE lines exhibited enhanced membrane fluidity and survival under cold, salt, and drought stresses, and demonstrated reduced lipid peroxidation and membrane leakage via activation of calcium signaling and suppression of ROS (Shi et al, 2018). As such, the sku5 line may require alternative pathways to be activated to maintain membrane fluidity and integrity during spaceflight acclimation due to the removal of SKU5 from this response pathway.…”

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

“…The endocytosis of SKU5 and other GPI-APs is modulated across the root tip by salt and auxin treatments, linking the movement of these proteins to stress responses (Baral et al, 2015). The expression of SKU5 is itself regulated by boron deprivation, cold, salt, and immune responses, as well as abscisic acid (ABA) treatment (Keinath et al, 2010;Elmore et al, 2012;Li et al, 2012;Tanaka et al, 2016;Shi et al, 2018;Miki et al, 2019). While SKU5 interactors are unknown in Arabidopsis, a maize homolog of SKU5 interacts with a C-terminal peptide of AUXIN-BINDING PROTEIN 1 (ABP1) in vitro (Shimomura, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

et al. 2020

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The observation that plant roots skew in microgravity recently refuted the long-held conviction that skewing was a gravity-dependent phenomenon. Further, spaceflight root skewing suggests that specific root morphologies and cell wall remodeling systems may be important aspects of spaceflight physiological adaptation. However, connections between skewing, cell wall modification and spaceflight physiology are currently based on inferences rather than direct tests. Therefore, the Advanced Plant Experiments-03-2 (APEX-03-2) spaceflight study was designed to elucidate the contribution of two skewing-and cell wall-associated genes in Arabidopsis to root behavior and gene expression patterns in spaceflight, to assess whether interruptions of different skewing pathways affect the overall spaceflight-associated process. SPIRAL1 is a skewingrelated protein implicated in directional cell expansion, and functions by regulating cortical microtubule dynamics. SKU5 is skewing-related glycosylphosphatidylinositolanchored protein of the plasma membrane and cell wall implicated in stress response signaling. These two genes function in different cellular pathways that affect skewing on the Earth, and enable a test of the relevance of skewing pathways to spaceflight physiological adaptation. In this study, both sku5 and spr1 mutants showed different skewing behavior and markedly different patterns of gene expression in the spaceflight environment. The spr1 mutant showed fewer differentially expressed genes than its Col-0 wild-type, whereas sku5 showed considerably more than its WS wild-type. Developmental age played a substantial role in spaceflight acclimation in all genotypes, but particularly in sku5 plants, where spaceflight 4d seedlings had almost 10-times as many highly differentially expressed genes as the 8d seedlings. These differences demonstrated that the two skewing pathways represented by SKU5 and SPR1 have unique and opposite contributions to physiological adaptation to spaceflight. The spr1 response is less intense than wild type, suggesting that the loss of SPR1 positively impacts spaceflight adaptation. Conversely, the intensity of the sku5 responses suggests that the loss of SKU5 initiates a much more complex, deeper and more stress related response to spaceflight. This suggests that proper SKU5 function is important to spaceflight adaptation.

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Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 57%

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

et al. 2020

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“…In herbaceous plants, increases in α‐linolenic acid levels may activate the plasma membrane Ca 2+ ‐ATPase, which is a crucial regulator to switch off the signal triggering ROS production. Activation of Ca 2+ ‐ATPase will enhance the ROS‐scavenging ability (Nicoletta et al , Shabala et al , Huda et al ) and increase the expression of stress‐responsive genes (Shi et al ). Previous study reveals that overexpression of ω 3 desaturases can increase the α‐linolenic acid contents and enhance drought tolerance of transgenic tobacco plants (Zhang et al ).…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolomics analysis reveals different metabolic responses to drought in tolerant and susceptible poplar species

Jia

Wang

et al. 2019

Physiologia Plantarum

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Drought is one of the critical factors limiting tree growth and survival. Clarifying the adaptation to drought will facilitate the cultivation of drought‐tolerant varieties. Metabolites, as direct signatures of biochemical functions, can uncover the biochemical pathways involved in drought responses. Here, we investigated the physiological and metabolic responses of drought‐tolerant Populus simonii and drought‐susceptible Populus deltoides cv. ‘Danhong’ to drought. Under drought conditions, P. simonii grew better and had a higher photosynthetic rate than P. deltoides cv. ‘Danhong’. Global untargeted metabolite profiling was analyzed using gas chromatography time‐of‐flight mass spectrometry system. A total of 69 and 53 differentially accumulated metabolites were identified in drought‐stressed P. simonii and P. deltoides cv. ‘Danhong’, respectively. The metabolisms of carbohydrate, amino acid, lipid and energy were involved in the drought responses common to both poplar species. The citric acid cycle was significantly inhibited to conserve energy, whereas multiple carbohydrates acting as osmolytes and osmoprotectants were induced to alleviate the adverse effects of drought stress. Unlike P. deltoides cv. ‘Danhong’, P. simonii underwent a specific metabolic reprogramming that enhanced non‐enzymatic antioxidants, coordinated the cellular carbon/nitrogen balance and regulated wax biosynthesis. These results provide a reference for characterizing the mechanisms involved in poplar response to drought and for enhancing the drought tolerance of forest trees.

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Genome‐wide identification of FAD gene family and functional analysis of MsFAD3.1 involved in the accumulation of α‐linolenic acid in alfalfa

et al. 2020

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As an important forage legume in the world, alfalfa (Medicago sativa L.) has high adaptability to various unfavorable climatic conditions and high biomass, and have been playing critical roles in animal husbandry and industrial applications. As α‐linolenic acid cannot be synthesized by animals, and most must be obtained from plants, the increasing of α‐linolenic acid content in alfalfa will greatly contribute to improve quality of livestock. However, the molecular mechanisms for α‐linolenic acid synthesis and accumulation in alfalfa are still limited. In this study, the importance of ω‐3 fatty acid desaturase (FAD) was demonstrated by analyzing α‐linolenic acid metabolic pathways, combined with the dynamics of accumulation of unsaturated fatty acids in alfalfa. Moreover, the FAD3.1 identified in alfalfa was located in the endoplasmic reticulum, and its expression level was consistent with the accumulation patterns of α‐linolenic acid in leaves. Heterologous expression in yeast cells proves that MsFAD3.1 was involved in the synthesis of α‐linolenic acid, and the α‐linolenic acid content in MsFAD3.1‐overexpression transgenic alfalfa lines was significantly increased. These results indicate that new alfalfa germplasm with high α‐linolenic acid content can be successfully created through biotechnology, providing a theoretical basis for further improving the quality of alfalfa and the nutritional value of dairy products.

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Integrated regulation triggered by a cryophyte ω-3 desaturase gene confers multiple-stress tolerance in tobacco

Abstract: A ω-3 fatty acid desaturase gene from Chorispora bungeana confers multi-stress tolerance by the integrated regulation of membrane, [Ca2+]cyt, ROS, and stress-responsive genes in tobacco

Cited by 41 publications

References 107 publications

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

Comparative metabolomics analysis reveals different metabolic responses to drought in tolerant and susceptible poplar species

Genome‐wide identification of FAD gene family and functional analysis of MsFAD3.1 involved in the accumulation of α‐linolenic acid in alfalfa

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