Integrated physiological and transcriptomic analysis reveals mechanism of leaf in Phellodendron Chinense Schneid seedlings response to drought stress

Zhang, Xintong; Sun, Wenkai; Chen, Xiaoyong; Chen, Lili; Lv, Zhencheng; He, Hanjie; Yan, Wende

doi:10.1016/j.indcrop.2023.116679

Cited by 3 publications

(3 citation statements)

References 67 publications

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“…Under drought treatment, 13 LHCB and one photosystem I subunit II ( PsaD ) unigenes were downregulated in sativa and falcata . In Phellodendron chinense Schneid, reduced expression of Pc(S) - Lhcb3/4/5/6 and PasD unigenes led to a decrease in LHCII complex and ΔpH, resulting in reduced NPQ . Additionally, transgenic Arabidopsis complementation lines for all lhcb mutants exhibited wild-type phenotypes in response to ABA, promoting stomatal closure and inhibiting stomatal opening, demonstrating that lhcb mutant phenotypes indeed resulted from downregulation or disruption of LHCB genes .…”

Section: Discussionmentioning

confidence: 96%

Multiomics Analyses Reveal MsC3H29 Positively Regulates Flavonoid Biosynthesis to Improve Drought Resistance of Autotetraploid Cultivated Alfalfa (Medicago sativa L.)

Dong,

Han,

Chen

et al. 2024

J. Agric. Food Chem.

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Section: Discussionmentioning

confidence: 96%

Multiomics Analyses Reveal MsC3H29 Positively Regulates Flavonoid Biosynthesis to Improve Drought Resistance of Autotetraploid Cultivated Alfalfa (Medicago sativa L.)

Dong,

Han,

Chen

et al. 2024

J. Agric. Food Chem.

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“…The pigment contents in the leaves of collected seedlings were measured according to the published literature [67]. Briefly, fresh leaves (0.2 g) of P. chinense Schneid seedlings were loaded into tubes that contained 10 mL 95% ethanol solution and placed in the dark condition for 24 h after sealing, then centrifuged at 11,000 rpm for 5 min.…”

Section: Pigment Content Measurementmentioning

confidence: 99%

Integrated Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Gibberellic Acid Regulates the Growth and Flavonoid Synthesis in Phellodendron chinense Schneid Seedlings

Yang,

Luo,

Jiao

et al. 2023

IJMS

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The phytohormone gibberellic acids (GAs) play a crucial role in the processes of growth, organ development, and secondary metabolism. However, the mechanism of exogenous GA3 regulating the growth and flavonoid synthesis in Phellodendron chinense Schneid (P. chinense Schneid) seedlings remains unclear. In this study, the physicochemical properties, gene expression level, and secondary metabolite of P. chinense Schneid seedlings under GA3 treatment were investigated. The results showed that GA3 significantly improved the plant height, ground diameter, fresh weight, chlorophyll content, soluble substance content, superoxide dismutase, and peroxidase activities. This was accompanied by elevated relative expression levels of Pc(S)-GA2ox, Pc(S)-DELLA, Pc(S)-SAUR50, Pc(S)-PsaD, Pc(S)-Psb 27, Pc(S)-PGK, Pc(S)-CER3, and Pc(S)-FBA unigenes. Conversely, a notable reduction was observed in the carotenoid content, catalase activity and the relative expression abundances of Pc(S)-KAO, Pc(S)-GID1/2, and Pc(S)-GH 3.6 unigenes in leaves of P. chinense Schneid seedlings (p < 0.05). Furthermore, GA3 evidently decreased the contents of pinocembrin, pinobanksin, isosakuranetin, naringin, naringenin, (−)-epicatechin, tricetin, luteolin, and vitexin belonged to flavonoid in stem bark of P. chinense Schneid seedlings (p < 0.05). These results indicated that exogenous GA3 promoted growth through improving chlorophyll content and gene expression in photosynthesis and phytohormone signal pathway and inhibited flavonoid synthesis in P. chinense Schneid seedlings.

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“…The P. chinense Schneid is an important traditional medicinal plant which mainly distributed in the elevation range of 800–2000 meters in subtropical evergreen broad-leaved forests of China ( He et al, 2020 ; Zhang et al, 2023 ). The stem and root barks of this plant are rich in various bioactive compounds such as berberine and phellodendrine, which widely uses for the clinic therapies of cancer, hepatitis, pneumonia, diabete and arthritis ( Sun et al, 2019 ; Rauf et al, 2021 ; Nematollahi et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

The response of nutrient cycle, microbial community abundance and metabolic function to nitrogen fertilizer in rhizosphere soil of Phellodendron chinense Schneid seedlings

Gu,

Chen,

Shen

et al. 2023

Front. Microbiol.

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Nitrogen (N) as an essential macronutrient affects the soil nutrient cycle, microbial community abundance, and metabolic function. However, the specific responses of microorganisms and metabolic functions in rhizosphere soil of Phellodendron chinense Schneid seedlings to N addition remain unclear. In this study, four treatments (CK, N5, N10 and N15) were conducted, and the soil physicochemical properties, enzyme activities, microbial community abundances and diversities, metabolism, and gene expressions were investigated in rhizosphere soil of P. chinense Schneid. The results showed that N addition significantly decreased rhizosphere soil pH, among which the effect of N10 treatment was better. N10 treatment significantly increased the contents of available phosphorus (AP), available potassium (AK), ammonium nitrogen (NH4+-N), nitrate nitrogen (NO3−-N) and sucrase (SU) activity, as well as fungal diversity and the relative expression abundances of amoA and phoD genes in rhizosphere soil, but observably decreased the total phosphorus (TP) content, urease (UR) activity and bacterial diversity, among which the pH, soil organic matter (SOM), AP, NH4+-N and NO3−-N were the main environmental factors for affecting rhizosphere soil microbial community structure based on RDA and correlation analyses. Meanwhile, N10 treatment notably enhanced the absolute abundances of the uracil, guanine, indole, prostaglandin F2α and γ-glutamylalanine, while reduced the contents of D-phenylalanine and phenylacetylglycine in rhizosphere soil of P. chinense Schneid seedlings. Furthermore, the soil available nutrients represented a significant correlation with soil metabolites and dominant microorganisms, suggesting that N10 addition effectively regulated microbial community abundance and metabolic functions by enhancing nutrient cycle in the rhizosphere soil of P. chinense Schneid seedlings.

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Integrated physiological and transcriptomic analysis reveals mechanism of leaf in Phellodendron Chinense Schneid seedlings response to drought stress

Cited by 3 publications

References 67 publications

Multiomics Analyses Reveal MsC3H29 Positively Regulates Flavonoid Biosynthesis to Improve Drought Resistance of Autotetraploid Cultivated Alfalfa (Medicago sativa L.)

Multiomics Analyses Reveal MsC3H29 Positively Regulates Flavonoid Biosynthesis to Improve Drought Resistance of Autotetraploid Cultivated Alfalfa (Medicago sativa L.)

Integrated Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Gibberellic Acid Regulates the Growth and Flavonoid Synthesis in Phellodendron chinense Schneid Seedlings

The response of nutrient cycle, microbial community abundance and metabolic function to nitrogen fertilizer in rhizosphere soil of Phellodendron chinense Schneid seedlings

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