Integrated osteoimmunomodulatory strategies based on designing scaffold surface properties in bone regeneration

Chen, Zhao; Xing, Fei; Zhou, Yuxi; Yu, Peiyun; Xu, Jiawei; Luo, Rong; Zhou, Changchun; Xiang, Zhou; Rommens, Pol Maria; Liu, Ming; Ritz, Ulrike

doi:10.1039/d3tb00727h

Cited by 3 publications

(2 citation statements)

References 197 publications

(240 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osteoimmunomodulation plays a pivotal role in osteointegration by regulating immune cell responses and subsequent bone cell behavior . Upon implantation, the innate immune system reacts to the implanted materials, creating an immune niche that ultimately determines the fate of implant-to-bone integration. , As such, materials with immunomodulatory properties that promote an anti-inflammatory and prohealing immune microenvironment are crucial for enhancing osteointegration. Among the earliest immune cells to interact with implants are macrophages, which direct the host immune response.…”

Section: Resultsmentioning

confidence: 99%

Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization

Asadi Tokmedash,

Min

2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although the interaction between synthetic materials and biological systems is well-recognized, the role of surface topographical cues in regulating the local osteoimmune microenvironmentspecifically, their impact on host tissue and immune cells, and their dynamic interactionsremains underexplored. This study addresses this gap by investigating the impact of surface topography on osteogenesis and immunomodulation. We fabricated MXene/hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, and microscale topographical patterns using our custom bottom-up patterning method. These engineered surfaces were employed to assess the behavior of osteoblast precursor cells and macrophage polarization. Our results demonstrate that MXene/HAP-coated surfaces with microscale crumpled topography significantly influence osteogenic activity and macrophage polarization: these surfaces notably enhanced osteoblast precursor cell spreading, proliferation, and differentiation and facilitated a shift in macrophages toward an anti-inflammatory, prohealing M2 phenotype. The observed cell responses indicate that the physical cues from the crumpled topographies, combined with the chemical cues from the MXene/HAP coatings, synergistically create a favorable osteoimmune microenvironment. This study presents the first evidence of employing MXene/HAP-multilayer coated surfaces with finely crumpled topography to concurrently facilitate osteogenesis and immunomodulation for improved implant-to-tissue integration. The tunable topographic patterns of these coatings coupled with a facile and scalable fabrication process make them widely applicable for various biomedical purposes. Our results highlight the potential of these multilayer coatings with controlled topography to improve the in vivo performance and fate of implants by modulating the host response at the material interface.

show abstract

Section: Resultsmentioning

confidence: 99%

Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization

Asadi Tokmedash,

Min

2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…50 Upon implantation, the innate immune system reacts to the implanted materials, creating an immune niche that ultimately determines the fate of implant-to-bone integration. 51,52 As such, materials with immunomodulatory properties that promote an anti-inflammatory and prohealing immune microenvironment are crucial for enhancing osteointegration. Among the earliest immune cells to interact with implants are macrophages, which direct the host immune response.…”

Section: Macrophage Response To Different Topographical Cuesmentioning

confidence: 99%

Designer micro/nano-crumpled MXene multilayer coatings accelerate osteogenesis and regulate macrophage polarization

Tokmedash,

Min

2024

Preprint

View full text Add to dashboard Cite

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although the interaction between synthetic materials and biological systems is well-recognized, the role of surface topographical cues in regulating the local osteoimmune microenvironment—specifically, their impact on host tissue and immune cells and their dynamic interactions—remains underexplored. This study addresses this gap by investigating the impact of surface topography on osteogenesis and immunomodulation. We fabricated MXene/Hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, and micro-scale topographical patterns using our custom bottom-up pattering method. These engineered surfaces were employed to assess the behavior of osteoblast precursor cells and macrophage polarization. Our results demonstrate that MXene/HAP-coated surfaces with microscale crumpled topography significantly influence osteogenic activity and macrophage polarization: These surfaces notably enhanced osteoblast precursor cell spreading, proliferation, differentiation, and facilitated a shift in macrophages towards an anti-inflammatory, pro-healing M2 phenotype. The observed cell responses indicate that the physical cues from the crumpled topographies, combined with the chemical cues from the MXene/HAP coatings, synergistically create a favorable osteoimmune microenvironment. This study presents the first evidence of employing MXene/HAP-multilayer coated surfaces with finely crumpled topography to concurrently facilitate osteogenesis and immunomodulation for improved implant-to-tissue integration. The tunable topographic patterns of these coatings, coupled with a facile and scalable fabrication process, make them widely applicable for various biomedical purposes. Our results highlight the potential of these novel coatings to improve thein vivoperformance and fate of implants by modulating the host response at the material interface.

show abstract

Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Lin,

Chen

et al. 2024

Preprint

View full text Add to dashboard Cite

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement to enhance bone regeneration and modulate the immune microenvironment to promote tissue repair. We synthesized a LiCS ceramic powder and performed comprehensive analyses of its physicochemical properties, including phase composition, morphology, setting time, and mechanical strength. The results demonstrated that the incorporation of lithium into calcium silicate significantly increased the diametral tensile strength (DTS) and facilitated hydroxyapatite formation compared with undoped calcium silicate. In vitro assays revealed that the LiCS cement enhanced the proliferation, adhesion, and spread of Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS cement exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines, promoting the polarization of macrophages towards the M2 phenotype. The presence of Li in the cement also significantly improved the osteogenic differentiation of WJMSCs, as evidenced by elevated levels of alkaline phosphatase and osteocalcin expression. These findings underscore the dual functional capabilities of the LiCS cement in enhancing osteogenesis and modulating the immune environment, making it a promising material for bone tissue engineering and regeneration.

show abstract

Integrated osteoimmunomodulatory strategies based on designing scaffold surface properties in bone regeneration

Abstract: Traditional biomaterials as bone substitutes always regard the immune response as an obstacle leading to implantation failure. However, cumulative evidence revealed that blindly minimizing host immune reactions cannot induce successful...

Cited by 3 publications

References 197 publications

Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization

Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization

Designer micro/nano-crumpled MXene multilayer coatings accelerate osteogenesis and regulate macrophage polarization

Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Contact Info

Product

Resources

About