Integrated Optical Density (IOD), Syntactic Structure Analysis, and Survival in Operated Lung Carcinoma Patients

Kayser, Klaus; Liewald, F.; Kremer, K; Tacke, Michael

doi:10.1016/s0344-0338(11)80898-8

Cited by 9 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thin sections from tissue blocks were stained with periodic acid-Schiff (PAS), Masson's trichrome (MT) and Jones methenamine (JM), and the glomerular profile numerical density per area [N A (glom) per mm 2 ], glomerular volume (V G , expressed in μm 3 ), mesangial matrix (MM, expressed in percentage), tubular metaplasia (TM, expressed in % of glomeruli), interstitial fibrosis (IF, expressed in percentage) and tubular atrophy (TA, expressed in percentage) were evaluated by using an image processing software (Image-Pro Plus version 6; Media Cybernetics, Silver Spring, Maryland, USA). The integrated optical density (IOD) [5] was employed to assess the immunohistochemical labeling that allowed to estimate: peritubular capillary density (PC D , endothelial immunostaining for CD34), peritubular capillary protection (T VEGF , tubular staining for vascular endothelial growth factor), apoptosis (tubular expression for caspase-3) and oxidative stress status (tubular expression for: thioredoxin-1 (T trx1 ) and peroxiredoxin-2 (T prx2 )). The normalized total kidney mass (KM T ) was estimated for comparison purposes.…”

mentioning

confidence: 99%

Carvedilol protects the peritubular capillaries and kidney structure in spontaneously hypertensive rats

Cao

Llambí

Ottaviano

et al. 2015

International Journal of Cardiology

View full text Add to dashboard Cite

mentioning

confidence: 99%

Carvedilol protects the peritubular capillaries and kidney structure in spontaneously hypertensive rats

Cao

Llambí

Ottaviano

et al. 2015

International Journal of Cardiology

View full text Add to dashboard Cite

“…There have been a few previous reports about the entropy of nuclei, 17,18 and the authors of those reports used the different nuclear features for the definition of entropy. We used the area of the nucleus, which has an advantage for the sectioning of specimens to make slides.…”

Section: Discussionmentioning

confidence: 99%

“…Second, to assess densities, an intrinsic control is needed, otherwise a dark or bright image will be misleading. Kayser et al used lymphocytes as a control in lung carcinoma; 17 however, lymphocytes are occasionally seen in astrocytic tumors. As for shapes, they are often assessed using the form factor, which is the ratio between the perimeter and the area of nuclei.…”

Section: Discussionmentioning

confidence: 99%

Conditional entropy as an indicator of pleomorphism in astrocytic tumors

Tanaka

Nakazato

2004

Neuropathology

View full text Add to dashboard Cite

The entropies of nuclear arrangements as an indicator of pleomorphism are assessed using a morphometric method. Sixty astrocytic tumors (grades I, II, III and IV; 15 cases each) were reviewed and analyzed. All slides were stained with HE and MIB-1 antibody. The MIB-1 labeling index (LI) was assessed by counting nuclei under a microscope. Images of HE-stained slides were digitized and segmented using the watershed algorithm. Then, six nuclear parameters were measured automatically: (i) the number of total nuclei in the image, (ii) percentage of total nuclear area in the image, (iii) the mean area of the nucleus, (iv) the standard deviation of the area of the nucleus, (v) the entropy of nuclear arrangement (Entropysimple), and (vi) conditional entropy of nuclear arrangement (Entropyconditional). Entropysimple was defined according to the area of the nucleus and Entropyconditional was defined according to both the area of the nucleus and the area of its neighboring nuclei. Image processing and image analysis were performed with public domain software developed in the laboratory. Segmentation of the images resulted in inappropriate segmentation in a few percent of the images. The measurements obtained for each parameter were classified using discriminant analysis. The percentage of correct classification with Entropyconditional was 62%, which was the highest value among all the measurements. Classification based on the combination of all measurements resulted in a rate of correct classification of 88%. Thus, conditional entropy of nuclear arrangement is useful for grading of astrocytic tumors and it is proposed as an indicator of pleomorphism.

show abstract

“…The intensity of myelin staining in white matter regions was measured as optical density. The optical density was calculated as the log of the intensity of the ROI divided by the intensity of the background [50]. The optical density value was normalized to the area (in μm 2 ) of the ROI.…”

Section: Histologymentioning

confidence: 99%

White Matter Alterations in <b><i>Fmr1</i></b> Knockout Mice during Early Postnatal Brain Development

Shi

Zhuo

et al. 2019

Dev Neurosci

View full text Add to dashboard Cite

Fragile X syndrome (FXS) is the most commonly inherited form of intellectual disability ascribed to the autism spectrum disorder. Studies with FXS patients have reported altered white matter volume compared to controls. The Fmr1 knockout (KO) mouse, a model for FXS, showed evidence of delayed myelination during postnatal brain development. In this study, we examined several white matter regions in the male Fmr1 KO mouse brain compared to male wild-type (WT) mice at postnatal days (PND) 18, 21, 30, and 60, which coincide with critical stages of myelination and postnatal brain development. White matter volume, T₂ relaxation time, and magnetization transfer ratio (MTR) were measured using magnetic resonance imaging and myelin content was determined with histological staining of myelin. Differences in the developmental accumulation of white matter and myelin between Fmr1 KO and WT mice were observed in the corpus callosum, external and internal capsules, cerebral peduncle, and fimbria. Alterations were more predominant in the external and internal capsules and fimbria of Fmr1 KO mice, where the MTR was lower at PND 18, then elevated at PND 30, and again lower at PND 60 compared to the corresponding regions in WT mice. The pattern of changes in MTR were similar to those observed in myelin staining and could be related to the altered protein synthesis that is a hallmark of FXS. While no significant changes in white matter volumes and T₂ relaxation time between the Fmr1 KO and WT mice were observed, the altered pattern of myelin staining and MTR, particularly in the external capsule, reflecting the abnormalities associated with myelin content is suggestive of a developmental delay in the white matter of Fmr1 KO mouse brain. These early differences in white matter during critical developmental stages may contribute to altered brain networks in the Fmr1 KO mice.

show abstract

Integrated Optical Density (IOD), Syntactic Structure Analysis, and Survival in Operated Lung Carcinoma Patients

Cited by 9 publications

References 10 publications

Carvedilol protects the peritubular capillaries and kidney structure in spontaneously hypertensive rats

Carvedilol protects the peritubular capillaries and kidney structure in spontaneously hypertensive rats

Conditional entropy as an indicator of pleomorphism in astrocytic tumors

White Matter Alterations in <b><i>Fmr1</i></b> Knockout Mice during Early Postnatal Brain Development

Contact Info

Product

Resources

About