Integrated morphology analysis, metabolomic analysis and gene expression to assess the quality of four adventitious roots lines of Glycyrrhiza uralensis Fisch

Lü, Jun; Liang, Wenxia; Li, Jianli; Wang, Shihui; Yao, Lu; Wang, Juan; Gao, Wenyuan

doi:10.1007/s11240-018-1446-1

Cited by 3 publications

(4 citation statements)

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“…It showed G. uralensis adventitious roots were similar to wild-type roots in microscopic characteristics. Furthermore, adventitious roots of G. uralensis also produced similar metabolite profiles, such as total flavonoid, glycyrrhizic acid, and glycyrrhetinic acid (Lu et al 2018a). The cultivation of adventitious roots of Fig.…”

Section: Discussionmentioning

confidence: 87%

“…MB root also displayed similar gene expression and metabolite profiles to wild-type roots of S3Y. For Glycyrrhiza uralensis Fisch., there were four kinds of adventitious root lines induced from different explants (Lu et al 2018a).…”

Section: Discussionmentioning

confidence: 99%

“…f. could also accumulate similar bioactive compounds with their wild-type roots, respectively (Ho et al 2018;Liu et al 2013;Miao et al 2014). Hence, it appears that microscopic techniques complemented with metabolite profiles and gene expression can be used to explore the alternative sources of medicinal plant biomass (Lu et al 2018a;Shohael et al 2014).…”

Section: Discussionmentioning

confidence: 99%

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Screening and evaluation of adventitious root lines of Panax notoginseng by morphology, gene expression, and metabolite profiles

Yao

Wang

Liang

et al. 2019

Appl Microbiol Biotechnol

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Panax notoginseng is a commonly used Traditional Chinese Medicine (TCM) and has diverse pharmacological activities with triterpenoid saponins as its main active ingredient. In the cultivation of P. notoginseng, continuous cropping is a serious problem, which could induce reduced productivity, low tuber quality, and plant mortality. With unique advantages of easy control, relative stability, high yields, tissue culture is widely used in the protection of TCM resources. In this study, we screened one adventitious root line, multi-branched (MB) root induced from wild-type roots of P. notoginseng, with a high yield of total triterpenoid saponins (17.92 mg/g). The morphology analysis showed that MB root had structure similar to that of wild-type roots, except for the highly branched phenotype. MB root also showed close gene expression levels and metabolite profiles, which were also similar to those of wild-type roots of Demonstration Park (S3Y). Pearson's correlation coefficient analysis confirmed the importance of key gene, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), and transcription factor, PnERF1, in regulating triterpenoid saponin biosynthesis in P. notoginseng. These results suggested that MB root possesses potential value in the largescale cultivation of P. notoginseng. KeywordsPanax notoginseng . Adventitious root . Microscopic analysis . Triterpenoid saponins . Gene expression various therapeutic effects, such as anti-tumor, anti-oxidant (Ng 2006), blood circulation promotion (Zhang et al. 2016), and neurological function improvement (Gao et al. 2012). P. notoginseng extract and its active constituents are also widely utilized in food, health products, and cosmetics (Wang et al. 2016). In China, circa 5000 tons of P. notoginseng is consumed annually (Duan et al. 2017;Liu et al. 2014). P. notoginseng, as a perennial plant, has a narrow ecological range mainly in Wenshan, Yunnan province of China (Dong et al. 2018). It is known that continuous cropping is a serious problem in cultivation of P. notoginseng. Continuous cropping has multiple adverse effects on P. notoginseng, such as productivity and tuber quality decrease. In severe cases, continuous cropping will lead to plant death (Hu et al. 2011;Liu et al. 2011). Crop r o t a t i o n i s a n e ff e c t i v e m e t h o d f o r r e p l a n t i n g P. notoginseng; however, it needs 8 years or more (Ma et al. 2006). Nowadays, arable soils for cultivation of P. notoginseng are becoming scarce (Dong et al. 2018). In addition, the region of origin (Guan et al. 2007), harvest time (Xia et al. 2017), and processing (Yu et al. 2013) are also influential on the quality of P. notoginseng.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Screening and evaluation of adventitious root lines of Panax notoginseng by morphology, gene expression, and metabolite profiles

Yao

Wang

Liang

et al. 2019

Appl Microbiol Biotechnol

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“…Compared to 30Y, the accumulation pattern of metabolites was different from that of the Group 2, but the difference was not significant among adventitious roots, such as Ro. In the adventitious root of Glycyrrhiza uralensis Fisch, PCA revealed that the metabolite compositions of AR lines are similar, while native roots was distinct (Lu et al, ). This showed that the ginsenoside contents would be affected by culture environment, or the biosynthetic pathway of ginsenosides would be differentially regulated in these Panax ginseng samples.…”

Section: Discussionmentioning

confidence: 99%

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

et al. 2019

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In the study, six adventitious root lines of Panax ginseng have been successfully established. HPLC‐ESI‐MS analysis showed that 20 ginsenosides were identified in root lines, notoginsenoside Fa and notoginsenoside R2 were not found in AR lines. In AR lines, the highest accumulation of total ginsenosides was obtained in five‐year main AR (24.87 mg/g). Principal component analysis classified root lines into three groups. Five‐year ginseng was mostly similar with five‐year main AR, five‐year rootlet AR, and four‐year rootlet AR in ginsenosides composition of group 1. Besides, gene expressions were consistent with the production of total ginsenosides, and correlation analysis revealed that total ginsenosides biosynthesis was significantly positively correlated with the gene expression of dammarenediol synthase. Five‐year rootlet AR showed the highest activity on ferric‐reducing antioxidant power test among samples. It provides a scientific evidence for the further exploitation and large‐scale production of P. ginseng. Practical applications This study provides valuable information for the commercial scale culture of ginseng adventitious roots. This report combines morphology, ginsenoside composition and content, gene expression, and ferric‐reducing antioxidant power test to evaluate the quality of P. ginseng adventitious root, and combined with principal component analysis to screen out the high yield and stable ginseng adventitious roots. It would be profitable to use adventitious root culture of P. ginseng instead of field cultivation.

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Photosynthetic Properties and Structure of Leaves of Licorice Seedlings in Response to Drought Stress

Zhang

Song

Yang

et al. 2022

j biobased mat bioenergy

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Licorice (Glycyrrhiza uralensis Fisch.) is a perennial herb that has high drought resistance, and is widely used in food, pharmaceutical and cosmetics industries. Understanding the response mechanism of licorice leaves to drought stress can help to develop drought-tolerant medicinal crops. In this experiment, the changes of photosynthetic parameters and structure of licorice leaves under drought stress were investigated. After 10 days of treatment with different soil containing water of 80, 65, 50 and 35%, the photosynthetic indexes, leaf surface stomata, glands morphology, leaf structure and chloroplast structure of licorice seedlings were measured respectively. The results showed that under light stress (LS), licorice adapted to drought stress by adjusting stomatal number, morphology and gland density, while severe stress (SS) inhibited the electron transfer between photosystems, leading to inactivation of the oxygen emitting complex, destruction of chloroplast structure and insufficient CO2 supply, resulting in the decrease of photosynthesis. This paper explains the response mechanism of licorice leaves to drought stress, which provides a foundation for further exploring its mechanism and improving the yield.

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Integrated morphology analysis, metabolomic analysis and gene expression to assess the quality of four adventitious roots lines of Glycyrrhiza uralensis Fisch

Cited by 3 publications

References 26 publications

Screening and evaluation of adventitious root lines of Panax notoginseng by morphology, gene expression, and metabolite profiles

Screening and evaluation of adventitious root lines of Panax notoginseng by morphology, gene expression, and metabolite profiles

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

Photosynthetic Properties and Structure of Leaves of Licorice Seedlings in Response to Drought Stress

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