Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe

Lawrence, Andrea L.; Hii, Sze Fui; Jírsová, Dagmar; Panáková, Lucia; Ionică, Angela Monica; Gilchrist, Katrina; Modrý, David; Mihalca, Andrei Daniel; Webb, Cameron E.; Traub, Rebecca J.; Šlapeta, Jan

doi:10.1016/j.vetpar.2015.03.029

Cited by 57 publications

(39 citation statements)

References 45 publications

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“…Previously, there has been only one report since 1930 on the presence of the oriental cat flea C. orientis in Iran (Farhang‐Azad, ). Although C. orientis has been considered a subspecies of C. felis , recent reports upgrade it to a separate species, which is confirmed by molecular analysis (Lawrence et al ., ) and morphological characteristics (Ménier & Beaucournu, ). C. orientis has been found more commonly in southern and eastern parts of Iran and has been regarded as a species belonging to East and Southeast Asia.…”

Section: Discussionmentioning

confidence: 99%

“…Given the specific role of each species and its ecology in the epidemiological chain of disease transmission, the accurate identification of flea species and their distributions is of particular importance. Molecular markers can be useful for confirming species identification based on morphological characteristics and have been applied to identify and characterize closely related flea species (Lawrence et al ., , ). One commonly used molecular marker for species identification and phylogenetic analysis of fleas is the cytochrome oxidase subunit I (COI) (Lawrence et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for the presence of Ctenocephalides orientis in livestock dwellings in northwest Iran

Seyyed-Zadeh

Bozorgomid

Telmadarraiy

et al. 2018

Medical Vet Entomology

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Fleas are important vectors of diseases such as murine typhus, tularaemia, hymenolepiasis and plague. The presence of active foci and history of human- and flea-transmitted plague in northwest Iran prompted the present group to collect and identify fleas from human and livestock dwellings across West Azerbaijan Province. Adult fleas were collected and identified using routine taxonomic keys. Species designation was confirmed by sequencing the cytochrome oxidase subunit I (COI). Of the collected specimens (n = 989), 104 were collected off-host (30 from human dwellings and 74 in light traps) and the rest were found on hosts (107 on animals and 778 by human bait). Of these fleas, 394 (40%) were male and 595 (60%) were female. The collected specimens belonged to the species Ctenocephalides canis, Ctenocephalides felis, Ctenocephalides orientis and Pulex irritans (all: Siphonaptera: Pulicidae). The amplified COI fragment, in addition to confirming the morphological identification of species, showed good efficacy in separating the different species in the phylogenetic analysis. In addition to the identification of fleas from human and livestock dwellings using morphological and molecular characteristics, the current paper represents the first report of the presence of C. orientis in northwest Iran. This finding suggests that changing climate conditions may have expanded the distribution of this species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence for the presence of Ctenocephalides orientis in livestock dwellings in northwest Iran

Seyyed-Zadeh

Bozorgomid

Telmadarraiy

et al. 2018

Medical Vet Entomology

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“…To this end, accurate identification of the arthropods is required, at the species level. Until recently, tick and flea species were identified using morphological criteria (Beaucournu & Launay, 1990;Walker et al, 2003) or molecular tools (Mediannikov & Fenollar, 2014;Lawrence et al, 2015). Although these methods have both demonstrated success in arthropod identification, they also have several drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI‐TOF MS

Nebbak

Hamzaoui

Bérenger

et al. 2017

Medical Vet Entomology

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Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months. The MS spectra were then compared with those obtained from manual pestle grinding, the standard homogenization method. Both automated methods generated intense, reproducible MS spectra from fresh specimens. Frozen storage methods appeared to represent the best preservation mode, for up to 6 months, while storage in ethanol is also possible, with some caveats for tick specimens. Carnoy's buffer, however, was shown to be less compatible with MS analysis for the purpose of identifying ticks or fleas. These standard protocols for MALDI-TOF MS arthropod identification should be complemented by additional MS spectrum quality controls, to generalize their use in monitoring arthropods of medical interest.

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“…Asimismo, pueden correr muy rápido y sus largas patas posteriores le permiten realizar saltos hasta de 30 cm de largo. Los adultos solo emergen de sus puparios hasta que detectan que el hospedador ha ingresado a su nido, lo que explica que en algunos domicilios que han permanecido desocupados por mucho tiempo, los nuevos ocupantes comienzan a «vivir con pulgas» (ESCCAP, 2012; Gunn y Pitt, 2012; Lawrence et al, 2015).…”

Section: Piojosunclassified

Microscopía y Principales Características Morfológicas de Algunos Ectoparásitos de Interés Veterinario

Pulido-Villamarín¹,

Castañeda-Salazar²,

Ibarra‐Avila³

et al. 2016

Rev. investig. vet. Perú

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RESUMENLos ectoparásitos afectan de manera importante el estado sanitario de los animales debido a que sus efectos exfoliatrices, tóxicos y alérgicos causan un detrimento en su bienestar y, adicionalmente, pueden tener un efecto negativo en los índices de conversión alimenticia en animales de producción. Las ectoparasitosis pueden afectar animales de todas las especies y edades; sin embargo, los jóvenes son los más susceptibles. La importancia de un adecuado diagnóstico de estos problemas sanitarios está dada no solo por la incomodidad que generan en sus hospederos, sino por la posibilidad que tienen de transmitir otros agentes patógenos. El objetivo del presente trabajo fue presentar imágenes donde se describen algunas características morfológicas de determinados ectoparásitos de interés en Medicina Veterinaria, especialmente bajo las condiciones de Colombia.Palabras clave: diagnóstico, parásitos externos, microscopía óptica, microscopía electrónica de barrido ABSTRACTThe ectoparasites significantly affect the health status of the animals due to their toxic and allergic effects that cause a detriment in their welfare and additionally may have a negative effect on feed conversion rates in farm animals. Ectoparasites can affect animals of all species and ages, being the young animals the most susceptible. The importance of a proper diagnosis of these health problems is given not only by the

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Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe

Cited by 57 publications

References 45 publications

Evidence for the presence of Ctenocephalides orientis in livestock dwellings in northwest Iran

Evidence for the presence of Ctenocephalides orientis in livestock dwellings in northwest Iran

Comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by MALDI‐TOF MS

Microscopía y Principales Características Morfológicas de Algunos Ectoparásitos de Interés Veterinario

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