Integrated molecular systems for biosensors

Aizawa, Masuo; Nishiguchi, Kenji; Imamura, Munenori; Kobatake, Eiry; Haruyama, Tetsuya; Ikariyama, Yoshihito

doi:10.1016/0925-4005(95)85005-8

“…Biosensors based on the genetic systems of bacteria that metabolize pollutants have been described (1,10,11,20,40,41). These biosensors have used the XylR or NahR proteins to activate expression of reporter genes.…”

Section: Discussionmentioning

confidence: 99%

“…The Pseudomonas putida XylR protein has served as the detection component for a number of biosensors (1,11,20,41) based on the ability of this protein to activate transcription in response to xylene and toluene. DmpR, the product of the Pseudomonas sp.…”

mentioning

confidence: 99%

“…Expression of the reporter gene provides a measurable response when the appropriate transcription activator protein interacts with an effector molecule to signal a particular environmental condition. Bacterial biosensors have been created to detect naphthalene (10), benzene derivatives, including toluene and xylene (1,11,20,41); and certain toxic metals (20,25).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Generation of Novel Bacterial Regulatory Proteins That Detect Priority Pollutant Phenols

Wise

¹

,

Kuske

²

2000

Appl Environ Microbiol

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The genetic systems of bacteria that have the ability to use organic pollutants as carbon and energy sources can be adapted to create bacterial biosensors for the detection of industrial pollution. The creation of bacterial biosensors is hampered by a lack of information about the genetic systems that control production of bacterial enzymes that metabolize pollutants. We have attempted to overcome this problem through modification of DmpR, a regulatory protein for the phenol degradation pathway of Pseudomonas sp. strain CF600. The phenol detection capacity of DmpR was altered by using mutagenic PCR targeted to the DmpR sensor domain. DmpR mutants were identified that both increased sensitivity to the phenolic effectors of wild-type DmpR and increased the range of molecules detected. The phenol detection characteristics of seven DmpR mutants were demonstrated through their ability to activate transcription of a lacZ reporter gene. Effectors of the DmpR derivatives included phenol, 2-chlorophenol, 2,4-dichlorophenol, 4-chloro-3-methylphenol, 2,4-dimethylphenol, 2-nitrophenol, and 4-nitrophenol.

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“…A glucose sensor based on a self-assembled monolayer of 4-aminothiophenol has also been described . The application of self-assembled monolayers of thiols for the design of modified electrodes and biosensors was recently reviewed by Mandler and Turyan and Aizawa et al…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Monolayers of Cystamine and Cysteamine on Gold Studied by XPS and Voltammetry

Wirde

¹

,

Gelius

²

,

Nyholm

³

1999

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The formation of self-assembled chemisorbed layers of cystamine, cysteamine, and 4-aminothiophenol on gold has been studied by XPS and voltammetry. These compounds, often used in the preparation of biosensors and modified electrodes, are shown to yield surface coverages of approximately 80% of that of a octadecanethiol monolayer within 5 min in millimolar aqueous and ethanolic solutions. The results of the XPS experiments reveal that a shoulder on the S 2p3/2 peak (situated at 162.1 eV) develops at 161.3 eV upon increasing the adsorption time from minutes to 1 week and that the initial rate of formation of the shoulder is higher for cystamine than for cysteamine. This shoulder is believed to be due to the presence of a sulfur species with a higher coordination number with respect to gold. Increased adsorption times also give rise to increased amounts of oxidized carbon and sulfur in the films. The oxidation of the sulfur in the thiols results in a detachment of the molecules from the gold surface, as indicated by XPS experiments with different takeoff angles. The main N 1s peak for cystamine is shifted toward higher binding energies for increasing adsorption times while two prominent nitrogen peaks are generally seen for cysteamine. For cysteamine, increasing adsorption times result in an increase of the main nitrogen component at the higher binding energy, yielding an apparent shift in the nitrogen peak with time similar to that seen for cystamine. Possible explanations for these experimental findings are discussed. Cystamine, cysteamine, and 4-aminothiophenol films on gold are shown to be irreversibly oxidized in the gold oxide formation region. On the basis of evaluation of the oxidation charge, surface coverages of approximately 1 × 10-9 mol/cm2 were obtained for adsorption times between 5 min and 1 week.

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“…In general, biosensors are classified into two main groups: bioaffinity and metabolism biosensors [332,333]. This reflects what happens between the receptor and the analyte.…”

Section: Optical Biosensors Biochips Miniaturized Analytical Systemsmentioning

confidence: 99%

Instruments in Biotechnology and Medicine

Voss¹,

Feller²,

Beckmann³

2012

Handbook of Biophotonics

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The sections in this article are Photonic Instruments in Medicine Introduction Optical Window of Cells and Tissues Laser Light–Matter Interaction as a Function of Intensity Photochemical Treatment of Tissues Thermal Processes in Light–Matter Interaction Nonlinear Processes in Light–Matter Interaction (Not to Be Confused with Nonlinear Optics) Minimally Invasive Diagnostics – Endoscopy History Types and Components of Endoscopes Advantages and Disadvantages of Endoscopy Application Fields Combination of Standard White Light Endoscopic with Other Diagnostic Methods Some Trends in Endoscopy Noninvasive Diagnostics – Photoplethysmography Introduction Characterization of the PPG Signal Signal Acquisition Clinical Applications Summary Noninvasive Diagnostics – Ophthalmology Introduction Corneal Topography Perimetry Optical Biometry Retinal Imaging and Glaucoma Diagnostics Fluorescein Angiography Wavefront Analysis In Vitro Diagnostics – Microscopy Light Microscope Fluorescence Microscope New Fluorescence Microscope Techniques Confocal Microscopy Raman Microscopy In Vitro Diagnostics – Digital Slides and Virtual Microscopy In Vitro Diagnostics – Optical Methods in Clinical Analysis System Optical Spectroscopy of Blood and Urine Components Optical Spectroscopy of Tissues and Vessels Optical Spectroscopy of Agglutination and Precipitation – Nephelometry, Turbidimetry In Vitro Diagnostics – Blood Gas Pressure Measurements Optical Detection of p O 2 in Blood Optical Detection of p CO 2 in Blood Optical Detection of Further Monitoring Parameters in Intensive Care (Temperature, p H ) In Vitro Diagnostics – Photoacoustic Spectroscopy Therapy Introduction Laser Therapy in Ophthalmology – LASIK (Refraction Correction by Cornea Treatment) Photonic Instruments in Biotechnology Introduction Selected Analytical Methods Introduction Reflectometric Interference Spectroscopy ( RIf S ) Surface Plasmon Resonance ( SPR ) Fluorescence Methods Advanced Microscopic Techniques (Selection) Enzyme‐Linked Immunosorbent Assay ( ELISA ) Optical Biosensors, Biochips, Miniaturized Analytical Systems High‐Throughput Screening ( HTS ) Sample Preparation: Flow Injection Analysis Fermentation as a Central Process of Biotechnology General Aspects Fermentation Control Photobioreactors Optical Trap/Optical Tweezers Basic Scheme of Laser Tweezers and Micromanipulation Applications of Optical Tweezers

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Integrated molecular systems for biosensors

Cited by 17 publications

References 1 publication

Generation of Novel Bacterial Regulatory Proteins That Detect Priority Pollutant Phenols

Generation of Novel Bacterial Regulatory Proteins That Detect Priority Pollutant Phenols

Self-Assembled Monolayers of Cystamine and Cysteamine on Gold Studied by XPS and Voltammetry

Instruments in Biotechnology and Medicine

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