Integrated microfluidic system for efficient DNA extraction using on-disk magnetic stirrer micromixer

Dehghan, Amin; Gholizadeh, Ali; Navidbakhsh, Mahdi; Sadeghi, Hossein; Pishbin, Esmail

doi:10.1016/j.snb.2021.130919

Cited by 23 publications

(15 citation statements)

References 40 publications

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“…To quantify mixing progress and evaluate the influence of effective parameters, the mixing process was video-recorded, and the captured video frames were imported into ImageJ for image processing. Similar to previous work, 39 the histogram of the grayscale intensity was obtained at different times, and the standard deviation was calculated as expressed below:where I i , Ī and N are the local pixel grayscale intensity value, the average of entire pixels intensity, and the total number of pixels, respectively. By calculating the standard deviation at the beginning ( σ max ) and the time of mixing completion ( σ min ), the mixing index at the time t was calculated as:…”

Section: Methodsmentioning

confidence: 99%

Section: Droplet Mixing Evaluationmentioning

confidence: 99%

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Robotic digital microfluidics: a droplet-based total analysis system

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Section: Droplet Mixing Evaluationmentioning

confidence: 99%

Robotic digital microfluidics: a droplet-based total analysis system

et al. 2023

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“…The magnetic slabs were attached to a rotating circular disk as magnetic stirrers, changing angular velocities from 0 to 480 rpm to investigate the shaking effect on mixing the DI-water with glycerol 75%. As a result of high mixing efficiency and production of viscous stresses, this platform was successfully implemented for cell lysis [ 90 ]. The following study presented a magnetofluid mixer for rapid mixing of ferrofluid and distilled water without producing harmful Joule heat up, when considering the microfluidic flow rate, magnet placement angle, and magnet dimensions.…”

Section: Microfluidicsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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“…[11][12][13] Active mixers rely mainly on applied force fields to enable faster sampling and higher mixing efficiency. [14][15][16][17] However, a typical drawback of this active mixing approach is the requirement for external instrumentation to provide the driving force, which undoubtedly increases the complexity of the structure of the micro-mixing system and increases the cost of detection. In contrast, passive micromixers only use the inertial forces caused by the fluid flow and microchannel structure to achieve mixing, which is simple, easy to manufacture, and highly integrated, and can better accommodate analytical instruments.…”

Section: Introductionmentioning

confidence: 99%