Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

He, Mei; Crow, Jennifer; Roth, Marc; Zeng, Yong; Godwin, Andrew K.

doi:10.1039/c4lc00662c

Cited by 418 publications

(340 citation statements)

References 62 publications

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“…Other methods, such as immunoaffinity isolations, are only used for small amounts of original sample because of the high price of the reagents required. Finally, microfluidic methods [45,53] are promising, with the possibility of being coupled to online analysis [54]. …”

Section: Semi-synthetic Exosomes: Biotechnological Modification Of Namentioning

confidence: 99%

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

García‐Manrique

Matos

Gutiérrez

et al. 2018

J of Extracellular Vesicle

133

139

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Extracellular vesicles (EVs) are emerging as novel theranostic tools. Limitations related to clinical uses are leading to a new research area on design and manufacture of artificial EVs. Several strategies have been reported in order to produce artificial EVs, but there has not yet been a clear criterion by which to differentiate these novel biomaterials. In this paper, we suggest for the first time a systematic classification of the terms used to build up the artificial EV landscape, based on the preparation method. This could be useful to guide the derivation to clinical trial routes and to clarify the literature. According to our classification, we have reviewed the main strategies reported to date for their preparation, including key points such as: cargo loading, surface targeting strategies, purification steps, generation of membrane fragments for the construction of biomimetic materials, preparation of synthetic membranes inspired in EV composition and subsequent surface decoration.

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Section: Semi-synthetic Exosomes: Biotechnological Modification Of Namentioning

confidence: 99%

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

García‐Manrique

Matos

Gutiérrez

et al. 2018

J of Extracellular Vesicle

133

139

View full text Add to dashboard Cite

show abstract

“…He et al captured EpCAM(+) EVs and incubated them with lysis buffer to obtain the cargo proteins [110]. They then analysed total IGF-1R and p-IGF-1R using an immuno-chemiluminescence assay after on-chip mixing with specific antibodies [110].…”

Section: Microfluidics-based Detection and Analysis Systemsmentioning

confidence: 99%

“…He et al captured EpCAM(+) EVs using magnetic Mf-IAC, and analysed the total IGF-1R and p-IGF-1R levels on-chip [110]. Shao et al performed chip-based EV isolation, RNA extraction and RT-qPCR in one device to detect and quantify multiple target mRNAs [85].…”

Section: Efforts and Progress Towards Integrated On-a-chip Systems Fomentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

131

100

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Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated “on-a-chip” systems. In addition, this review also discusses the potential clinical applications of “on-a-chip” systems, including both “liquid biopsies” for personalized medicine and DDS devices for precision medicine, and then anticipates the possible future participation of cloud-based portable disease diagnosis and monitoring systems, possibly with the participation of artificial intelligence (AI).

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“…Similar to CTCs, exosomes carry surface markers from the cell of origin, which is exploited for enrichment strategies [147]. Rapid advancement of another novel method known as nanoscale fluorescence activated cell sorting call, or nanoFACS, has further advanced the methods of exosome isolation and sorting and allowed for the study of discrete, free, individual exosomes from bodily fluids [148].…”

Section: Methods To Isolate and Identify Exosomesmentioning

confidence: 99%

Liquid biopsy - emergence of a new era in personalized cancer care

2018

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The most successful treatment for cancer involves identifying druggable, biological markers for targeted therapy. In the clinical setting, surgical removal of tumors is the only procedure for identifying such targetable molecules. Shed from tumor cells, these markers are also present in circulating blood, albeit in very negligible amounts. Liquid biopsy is a procedure performed on a blood sample to look for such circulating cancer markers cells or pieces of nucleic acid from the tumor. The procedure shows promise in revolutionizing personalized cancer treatments. Here we briefly review the technique, characterization, and its utilization in clinics.

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Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

Abstract: A microfluidic technology for integrated exosome analysis allows for specific immunoisolation, subpopulation profiling, and targeted proteomic analysis of circulating exosomes, with minimally invasive sample consumption and markedly improved assay speed and sensitivity.

Cited by 418 publications

References 62 publications

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

Therapeutic biomaterials based on extracellular vesicles: classification of bio‐engineering and mimetic preparation routes

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Liquid biopsy - emergence of a new era in personalized cancer care

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