Integrated evaluation the antioxidant activity of epicatechin from cell dynamics

Abstract

Oxidative damage has been implicated in the pathogenesis of numerous disorders by affecting the normal functions of several tissues. Further, oxidative stress acts within cells to influence cell morphology and the behavior of cell migration. The movement and migration of cells are crucial during the development of organisms as they transition from embryo to adult, and for the homeostasis of adult tissues. Epicatechin (EC) is a natural flavonoid derived mostly from tea, chocolate, and red wine. We investigated the protective impact of EC on D‐galactose(D‐gal)/rotenone‐injured NIH3T3 cells and found alterations in cell dynamics throughout the procedure. The results reveal that D‐gal/rotenone stimulation can cause the cell area to expand and the number of cellular protrusions to increase. EC intervention can considerably minimize the oxidative damage of rotenone on NIH3T3 cells (p < 0.05) but showed little influence on cell damage induced by D‐gal. Furthermore, the corrective ability of EC as an antioxidant is reflected in a dose‐dependent effect on cell movement, including variations in movement speed and distance. Overall, from the perspective of cell morphology and cell motility, EC has a good protective impact on cells harmed by rotenone induced oxidative damage, as well as corrective properties as an antioxidant to balance intracellular oxidative stress, which allowing for a more comprehensive evaluation of antioxidant performance of EC.

Fuji apple (Malus domestica Borkh. cv. Red Fuji) pomace extracts as a source of antimicrobial and antioxidant polyphenols

“…However, when the concentration increased to 200 μg/mL, the ABTS radical scavenging rates of both PPE and L-ascorbic acid were about 99.99%, which were five times of that of CPE, from which demonstrated the strong antioxidant activity of PPE. In conclusion, both PPE and CPE showed strong ABTS radical scavenging capacity, from which PPE being more potent, which could be due to its higher content of polyphenols, mainly including chlorogenic acid, epicatechin and catechin (Qing et al, 2023;Serra et al, 2021).…”

Evaluating the Antioxidant Properties of the Leaves and Stems of Alpinia oxyphylla In Vitro and Its Growth-Promoting, Muscle Composition Change, and Antioxidative Stress Function on Juvenile Litopenaeus vannamei

“…The epicatechin unit presented significant effects due to the structural features of ortho-dihydroxy at B ring and hydroxyl groups at positions 3 and 5, which are helpful for electron delocalization and provide hydrogen bonding with the oxo group [24]. Moreover, epicatechin (2) has been reported as a potent natural antioxidant [25] with hepatocyte protective [26], cardiovascular protective [27], and anti-aging effects [28] previously. Therefore, epicatechin (2) may be one of the important antioxidative chemical compositions in AOE.…”