Integrated data-driven modeling and experimental optimization of granular hydrogel matrices

Verheyen, Connor A.; Uzel, Sebastien G. M.; Kurum, Armand; Roche, Ellen T.; Lewis, Jennifer A.

doi:10.1016/j.matt.2023.01.011

Cited by 15 publications

(13 citation statements)

References 77 publications

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“…2E), indicating the extrusion of the liquid phase and no yielding or flow of the AlgOx microgel-only slurry. 55,58 Inspecting the compressed microgel plug after recovery from the syringe barrel confirmed these observations (Fig. S5B, ESI †).…”

Section: Biomaterials Science Papersupporting

confidence: 62%

“…For inks with low (or no) extrudability, the sample commonly resembled a pendant droplet that formed as the liquid phase of the ink was dispensed without successful rearrangement or flow of the constituent microgels. 55,58 As extrudability increased, the microgel slurry yielded to initiate flow through the nozzle and filament formation. Meanwhile, the filament became more cohesive, increasing the hanging length.…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, the filament became more cohesive, increasing the hanging length. 55,58 The mean hanging filament length for each ink is shown in Fig. 2B.…”

Section: Biomaterials Science Papermentioning

confidence: 99%

“…While other methods have been used to produce alginate-based microgels with reduced dispersity, including microfluidic devices and airflow-mediated droplet generation, these strategies require specialized equipment and extensive troubleshooting to maximize microgel reproducibility and cell viability. 33,[52][53][54][55] In contrast, the centrifugal microdroplet device utilized in this study can be created in one afternoon using standard laboratory consumables and tools available at most hardware stores. 47 The ease of setup and facile scalability of this centrifugal approach suited our need to rapidly create multi-milliliter-scale batches of jammed, cell-containing AlgOx microgels for 3D printing.…”

Section: Design Of Granular Gelma : Algox Microgel Inksmentioning

confidence: 99%

Section: Extrudability and Stability Of Gelma : Algox Microgel Inksmentioning

confidence: 99%

See 4 more Smart Citations

3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

Seymour,

Kilian,

Navarro

et al. 2023

Biomater. Sci.

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show abstract

Section: Biomaterials Science Papersupporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, the filament became more cohesive, increasing the hanging length. 55,58 The mean hanging filament length for each ink is shown in Fig. 2B.…”

Section: Biomaterials Science Papermentioning

confidence: 99%

Section: Design Of Granular Gelma : Algox Microgel Inksmentioning

confidence: 99%

Section: Extrudability and Stability Of Gelma : Algox Microgel Inksmentioning

confidence: 99%

See 3 more Smart Citations

3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

Seymour,

Kilian,

Navarro

et al. 2023

Biomater. Sci.

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Granular Hydrogels in Biofabrication: Recent Advances and Future Perspectives

Daly

2023

Adv Healthcare Materials

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Granular hydrogels, which are formed by densely packing microgels, are promising materials for bioprinting due to their extrudability, porosity, and modularity. However, the multidimensional parameter space involved in granular hydrogel design makes material optimization challenging. For example, design inputs such as microgel morphology, packing density, or stiffness can influence multiple rheological properties that govern printability and the behavior of encapsulated cells. This review provides an overview of fabrication methods for granular hydrogels, and then examines how important design inputs can influence material properties associated with printability and cellular responses across multiple scales. Recent applications of granular design principles in bioink engineering are described, including the development of granular support hydrogels for embedded printing. Further, the paper provides an overview of how key physical properties of granular hydrogels can influence cellular responses, highlighting the advantages of granular materials for promoting cell and tissue maturation after the printing process. Finally, potential future directions for advancing the design of granular hydrogels for bioprinting are discussed.

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Granular Hydrogels Improve Myogenic Invasion and Repair after Volumetric Muscle Loss

Tanner,

Schiltz,

Narra

et al. 2024

Adv Healthcare Materials

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Skeletal muscle injuries including volumetric muscle loss (VML) lead to excessive tissue scarring and permanent functional disability. Despite high prevalence, there is currently no effective treatment for VML. Bioengineering interventions such as biomaterials that fill the VML defect to support cell and tissue growth are a promising therapeutic strategy. However, traditional biomaterials developed for this purpose lack the pore features needed to support cell infiltration. The present study investigates for the first time, the impact of granular hydrogels on muscle repair – hypothesizing that their flowability will permit conformable filling of the defect site and their inherent porosity will support the invasion of native myogenic cells, leading to effective muscle repair. We prepared small and large microparticle fragments from photocurable hyaluronic acid polymer via extrusion fragmentation and facile size sorting. In assembled granular hydrogels, particle size and degree of packing significantly influenced pore features, rheological behavior, and injectability. Using a mouse model of volumetric muscle loss (VML), we demonstrate that, compared to bulk hydrogels, granular hydrogels support early‐stage (satellite cell invasion) and late‐stage (myofiber regeneration) muscle repair processes. Together, these results highlight the promising potential of injectable and porous granular hydrogels in supporting endogenous repair after severe muscle injury.This article is protected by copyright. All rights reserved

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Integrated data-driven modeling and experimental optimization of granular hydrogel matrices

Cited by 15 publications

References 77 publications

3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

3D printing microporous scaffolds from modular bioinks containing sacrificial, cell-encapsulating microgels

Granular Hydrogels in Biofabrication: Recent Advances and Future Perspectives

Granular Hydrogels Improve Myogenic Invasion and Repair after Volumetric Muscle Loss

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