Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfilter Chambers

Wilding, Peter; Kricka, Larry J.; Cheng, Jing; Hvichia, Gia; Shoffner, Mann A.; Fortina, Paolo

doi:10.1006/abio.1997.2530

Cited by 284 publications

(211 citation statements)

References 29 publications

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“…[8][9][10] With the development of microchip methods for RNA or DNA sample preparation, 11-13 PCR 14-17 and on-chip hybridization, 18,19 it has become clear that integrating the cell capture and concentration steps within a m-TAS system remains a bottleneck. Microfiltration 20 and dielectrophoresis 11,21 have been used to integrate blood sample preparation with PCR on microfabricated devices. These studies showed the challenge associated with blood sample preparation for PCR.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

2004

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Section: Introductionmentioning

confidence: 99%

Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

2004

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“…To minimize the surface affinity for molecules from the solution, we over-coated micro-wells with an additional silicon oxide dielectric layer 200 nm thick, by magnetron sputtering [24][25][26], forming titanium-silicon oxide coated and aluminum-silicon oxide coated fiber-optic slides. The surface morphologies of the four different coatings were examined by atomic force microscopy (AFM).…”

Section: Surface Coatingmentioning

confidence: 99%

Improved picoliter-sized micro-reactors for high-throughput biological analysis

Han

Yuan

Wei

et al. 2013

Sci. China Life Sci.

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High-throughput pyrosequencing, carried out in millions of picoliter-sized reactors on a fiber-optic slide, is known for its longer read length. However, both optical crosstalk (which reduces the signal-to-noise ratio of CCD images) and chemical retention adversely affect the accuracy of chemiluminescence determination, and ultimately decrease the read length and the accuracy of pyrosequencing results. In this study, both titanium and oxidized aluminum films were deposited on the side walls and upper faces of micro-reactor slides to enhance optical isolation; the films reduced the inter-well crosstalk by one order of magnitude. Subsequently, chemical retention was shown to be caused by the lower diffusion coefficient of the side walls of the picolitersized reactors because of surface roughness and random pores. Optically isolated fiber-optic slides over-coated with silicon oxide showed smoother surface morphology, resulting in little chemical retention; this was further confirmed with theoretical calculations. Picoliter-sized micro-reactors coated with titanium-silicon oxide films showed the least inter-well optical crosstalk and chemical retention; these properties are expected to greatly improve the high-throughput pyrosequencing performance.high throughput analysis, picoliter-sized micro-reactor, surface coating Citation:Han W J, Yuan L N, Wei Q Q, et al. Improved picoliter-sized micro-reactors for high-throughput biological analysis.

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“…Separation methods using fluid mechanical effects may be classified into two categories: micro filter devices [2][3] and microchannel devices [4][5]. Among the latter, channel constriction [6][7], bending channel [8][9] and bifurcated channels [10][11] have been explored.…”

Section: Introductionmentioning

confidence: 99%

Effect of fluid dynamics and device mechanism on biofluid behaviour in microchannel systems: Modelling biofluids in a microchannel biochip separator

Xue

Patel

Kersaudy-Kerhoas

et al. 2009

2009 International Conference on Electronic Packaging Technology &Amp; High Density Packaging

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Biofluid behaviour in microchannel systems is investigated in this paper through the modelling of a microfluidic biochip developed for the separation of blood plasma. Based on particular assumptions, the effects of some mechanical features of the microchannels on behaviour of the biofluid are explored. These include microchannel, constriction, bending channel, bifurcation as well as channel length ratio between the main and side channels. The key characteristics and effects of the microfluidic dynamics are discussed in terms of separation efficiency of the red blood cells with respect to the rest of the medium. The effects include the Fahraeus and Fahraeus-Lindqvist effects, the Zweifach-Fung bifurcation law, the cell-free layer phenomenon. The characteristics of the microfluid dynamics include the properties of the laminar flow as well as particle lateral or spinning trajectories. In this paper the fluid is modelled as a single-phase flow assuming either Newtonian or Non-Newtonian behaviours to investigate the effect of the viscosity on flow and separation efficiency. It is found that, for a flow rate controlled Newtonian flow system, viscosity and outlet pressure have little effect on velocity distribution. When the fluid is assumed to be Non-Newtonian more fluid is separated than observed in the Newtonian case, leading to reduction of the flow rate ratio between the main and side channels as well as the system pressure as a whole.

show abstract

Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfilter Chambers

Cited by 284 publications

References 29 publications

Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

Improved picoliter-sized micro-reactors for high-throughput biological analysis

Effect of fluid dynamics and device mechanism on biofluid behaviour in microchannel systems: Modelling biofluids in a microchannel biochip separator

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