Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

Furdui, Vasile I.; Harrison, David

doi:10.1039/b409366f

Cited by 142 publications

(128 citation statements)

References 28 publications

(42 reference statements)

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“…This technique can be used to separate components from the bulk fluid efficiently. These processes can be exquisitely sensitive and are well-suited to sorting rare cell types; and they are discussed comprehensively elsewhere (Furdui & Harrison, 2004;Pamme, 2006). With beads as a reagent carrier, the microfluidic system becomes much more adaptable and resource-conscious.…”

Section: Assay Designmentioning

confidence: 99%

Design Principles for Microfluidic Biomedical Diagnostics in Space

Nelson¹

2011

Biomedical Engineering - From Theory to Applications

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Section: Assay Designmentioning

confidence: 99%

Design Principles for Microfluidic Biomedical Diagnostics in Space

Nelson¹

2011

Biomedical Engineering - From Theory to Applications

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“…Microfluidic systems are likely to incorporate components such as magnetic cell sorting [143], FACS [144] and optical trapping [145]. In principle, any assay or experimental system can be miniaturized and customized for specific purposes.…”

Section: Microfluidicsmentioning

confidence: 99%

Shine a light: Imaging the immune system

Sarris

Betz

2009

Eur J Immunol

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Intra vital microscopy and whole-body imaging promise to revolutionize how we study the immune system. They compel by the intrinsic beauty of the images obtained and the undeniable direct biological relevance of the observations. However, it is important to remember that in many cases, fundamental insights into the underlying biological processes have already been obtained using ex vivo reductionist approaches. Indeed, it is likely that with the advent of microfluidics, new and exciting avenues will open up for ex vivo experimentation. Here, we give a brief but comprehensive overview of the various imaging techniques available, their relative strengths and shortcomings and how these tools have been used to get us to where we are today. The challenge for the future will be to apply the most suitable technology and to integrate the findings across various imaging disciplines to build a unified, comprehensive ''big picture'' of the immune system.Key words: Imaging . Microscopy . Technology During a comparative study of digestive organs, Elie Metchnikoff was intrigued by the fact that certain cells that play no role in digestion, nevertheless have the ability to ingest foreign bodies [1]. The ''model system'' he used to study this phenomenon was the starfish larva, which is transparent enough to observe individual cells moving within. Metchnikoff observed that when he inserted a small splinter into the larvae, some cells accumulated at the point of insult and tried to ingest the foreign body. He had discovered phagocytosis, a phenomenon he demonstrated to be ubiquitous throughout the Animal Kingdom [2]. As higher organisms were not suited to direct microscopic examination, Metchnikoff proceeded to isolate phagocytic cells from the blood of higher organisms and demonstrated that these cells ingest and destroy microorganisms. Based on his observation, he introduced the concept of cellular immunity. Thus, it is fair to say that microscopic imaging has always been at the center of immunological research from its very conception. Initially however, immunologists had no tools with which to observe the cells involved in immune responses in higher organisms in vivo. They could use either tissue sections to reconstitute what had happened from snapshots or they could study specific aspects of the immune response with isolated cell populations ex vivo. The contributions of tissue imaging in immunology over the past century have been recently reviewed [3]. Here, we provide a broad overview of the multitude of imaging approaches that have been applied in immunology, ranging from ex vivo single molecule detection to in vivo whole-body imaging and microfluidic ''lab on a chip'' technology for immunological studies. Ex vivo studiesMany of the functions of the immune system can be mimicked ex vivo using primary cell populations. Early separation techniques relied on the physical characteristics of the cells. Velocity sedimentation was used to sort the cells according to size [4] and differential adherence in order to separate subpop...

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“…In the past few years, several microfluidic based magnetic sorting concepts have been investigated to capture magnetic beads or magnetically labeled cells. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] However, these devices are generally hampered by complex fabrication processes and low volumetric throughputs. In many earlier studies, flow rates were limited to less than 1 ml/h, 9,10 which are not practical for many realworld applications.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the throughput of the chip is significantly higher than the recently developed microfluidic devices. 9,10 Furthermore, other unique features of our chip, such as microscale field gradients and continuous flow with a buffer switching scheme enhance the purity and recovery of the target cells. Similar approaches have been demonstrated to locally concentrate the gradient of the applied magnetic field by integrating an array of NiFe (80% Ni and 20% Fe) microneedles 24 and Ni microstructures 25 to one side of a microfluidic channel.…”

Section: Introductionmentioning

confidence: 99%

On-chip magnetophoretic isolation of CD4 + T cells from blood

Darabi

Guo

2013

Biomicrofluidics

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This paper presents the design, fabrication, and testing of a magnetophoretic bioseparation chip for the rapid isolation and concentration of CD4 þ T cells from the peripheral blood. In a departure from conventional magnetic separation techniques, this microfluidic-based bioseperation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. Additionally, the chip is capable of processing significantly smaller sample volumes than conventional methods and sample losses are eliminated due to decreased handling. Furthermore, the possibility of sample-to-sample contamination is reduced with the disposable format. The overall dimensions of the device were 22 mm by 60 mm by 1 mm, approximately the size of a standard microscope slide. The results indicate a cell purity of greater than 95% at a sample flow rate of 50 ml/h and a cell recovery of 81% at a sample flow rate of 10 ml/h. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A parametric study was also performed to investigate the effects of channel height, substrate thickness, magnetic bead size, and number of beads per cell on the cell separation performance. V C 2013 AIP Publishing LLC. [http://dx

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Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

Cited by 142 publications

References 28 publications

Design Principles for Microfluidic Biomedical Diagnostics in Space

Design Principles for Microfluidic Biomedical Diagnostics in Space

Shine a light: Imaging the immune system

On-chip magnetophoretic isolation of CD4 + T cells from blood

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