Integrated Biosensor Systems for Ethanol Analysis

Abstract

Different integrated systems with a bi-enzymatic biosensor, working with two different methods for ethanol detection--flow injection analysis (FIA) or sequential injection analysis (SIA)--were developed and applied for ethanol extracted from gasohol mixtures, as well as for samples of alcoholic beverages and fermentation medium. A detection range of 0.05-1.5 g ethanol/l, with a correlation coefficient of 0.9909, has been reached when using FIA system, working with only one microreactor packed with immobilized alcohol oxidase and injecting free horseradish peroxidase. When using both enzymes, immobilized separately in two microreactors, the detection ranges obtained varied from 0.001 to 0.066 g ethanol/l, without on-line dilution to 0.010-0.047 g ethanol/l when a 1:7,000 dilution ratio was employed, reaching correlation coefficients of 0.9897 and 0.9992, respectively. For the integrated biosensor SIA system with the stop-flow technique, the linear range was 0.005-0.04 g/l, with a correlation coefficient of 0.9922.

Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system

“…AOD and HRP (Toyobo of Brazil) were immobilized separately on aminopropyl glass beads treated with 2.5% (v/v) glutaraldehyde as previously described (4). AOD and HRP (Toyobo of Brazil) were immobilized separately on aminopropyl glass beads treated with 2.5% (v/v) glutaraldehyde as previously described (4).…”

“…Several analytical techniques that use enzymatic reactions have been applied to quantify low ethanol concentrations. FIA has been used for ethanol detection in alcoholic beverages and in gasohol mixtures (3)(4)(5). FIA has been used for ethanol detection in alcoholic beverages and in gasohol mixtures (3)(4)(5).…”

A análise por injeção sequencial (SIA): vinte anos em uma perspectiva brasileira

“…Há ainda trabalhos visando a determinação de etanol em bebidas alcoólicas e em bioprocessos. 63,66 Considerando-se metais na área industrial, foram realizados trabalhos envolvendo a determinação dos mesmos em banhos de eletrodeposição (Cr 6+ , Ni 2+ ) 20,46 e aço (Cr 6+ ) 32 ou latão (Cu 2+ , Zn 2+ , Pb 2+ ). 48 Um destes trabalhos 46 é um dos poucos (dentre os apontados na Tabela 2) a tratar da determinação de mais de uma característica da amostra por mais de um sistema de detecção distinto (potenciometria para pH e Cl -e espectrofotometria para Ni 2+ ).…”

Characterization of an hrp-aox-polyaniline-graphite composite biosensor

“…Immobilization of the biological material on the electrode surface constitutes a crucial step in development of the biosensor, since the enzyme's structure must be maintained in order to enable its action on the sample of interest [16][17][18]. Horseradish peroxidase (HRP) is widely used in enzyme-linked biosensors.…”