Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system

Alhadeff, Eliana M.; Salgado, Andréa Medeiros; Cós, Oriol; Pereira, Nei; BelkisValdman,; Valero, Francisco

doi:10.1007/s12010-007-9036-4

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…In addition, it is possible to select and manage up to two wavelengths, scanning, and monitoring at wavelengths in the range of 345-1000 nm, and real-time graphs of the variables measured; all this is presented in a friendly, versatile user interface which is easy to manipulate. This is relevant since the new trends in bioprocess are related to the use of micro bioreactors for enzymatic determination [55,56], which will require samplings with less volume from the culture medium. On the other hand, it will be required to monitor more complex bioprocesses with multiple metabolites to draw information about the physiological state of the cells [57,58], with the SIA system being a strategic tool to develop better control algorithms.…”

Section: Discussionmentioning

confidence: 99%

Monitoring β-Fructofuranosidase Activity through Kluyveromyces marxianus in Bioreactor Using a Lab-Made Sequential Analysis System

Barbosa-Hernández,

Pliego-Sandoval,

Gschaedler-Mathis

et al. 2023

Fermentation

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The yeast Kluyveromyces marxianus has shown the potential to produce β-fructofuranosidases, which are enzymes capable of hydrolyzing β-fructofuranosides links of fructans to obtain fructooligosaccharides. The thriving market for fructose syrup and the quality standards imposed by food and pharmaceutical industries have generated an increased search for improved, monitored, and controlled production processes. Monitoring β-fructofuranosidase activity in a bioprocess requires the use of adequate sensors and the processing of information using efficient software algorithms; nevertheless, currently, such a sensor does not exist for this purpose. In this contribution, a sequential injection analysis system (SIA) developed in our laboratory was adapted to monitor at-line β-fructofuranosidase activity produced by the yeast K. marxianus. Samples were taken out automatically from the bioreactor and analyzed using 3,5-dinitrosalicylic (DNS). An algorithm was designed to operate the overall components of the lab-made SIA system. The enzymatic activity error obtained with the automatic SIA compared to the off-line laboratory determinations varied from 0.07% at high enzyme concentrations to 20.39% at low β-fructofuranosidase activity. Further development is required to improve the performance of the lab-made SIA system; nevertheless, such a device must be considered as a potential method for monitoring β-fructofuranosidase activity in real time.

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Section: Discussionmentioning

confidence: 99%

Monitoring β-Fructofuranosidase Activity through Kluyveromyces marxianus in Bioreactor Using a Lab-Made Sequential Analysis System

Barbosa-Hernández,

Pliego-Sandoval,

Gschaedler-Mathis

et al. 2023

Fermentation

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Enzymatic Kinetic Determinations

Hüttl

Frank

2000

Encyclopedia of Analytical Chemistry

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Kinetic methods for analytical applications can be divided into noncatalytic and catalytic methods. Enzyme kinetic determinations are a special type of catalytic methods. Enzymatic reactions are used analytically to determine enzyme activities – for instance, in the diagnosis of diseases, substrate concentrations in the food industry or medicine, and concentrations of effectors – for example, in trace analysis. Owing to the high selectivity of enzymatic analysis, the importance of this method in various fields is growing rapidly. The widest use is observed in clinical chemistry and food chemistry. This article gives only a general survey of the high potential of enzymatic analysis, with references to more detailed literature. Enzymatic reactions are characterized by some special features, explained after the introduction. The theoretical background for kinetic determination is the comprehension of the principles on enzymatic kinetics. In Section 3, the simplest mechanism, outlined by Michaelis and Menten, and several transformations of the equation are described. In this section, two kinetic parameters, enzyme activity and the Michaelis constant, are introduced. Section 4 deals with substances, the so‐called effectors , influencing the rate of an enzymatic reaction. Simple mechanisms of these effectors are shown. Different methods for determination of substrates, enzymes, and effectors, illustrated by many examples, are represented in Section 5. While substrate concentrations can be determined using both equilibrium or kinetic methods, the determination of enzymes and effectors can be performed only by kinetic approaches. In the last two sections, the application of enzymes in flow systems and biosensor development are shown.

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