Integrated bioethanol production from mixtures of corn and corn stover

Chen, Sitong; Xu, Zhaoxian; Li, Xiujuan; Yu, Jianming; Cai, Mufeng; Jin, Mingjie

doi:10.1016/j.biortech.2018.02.125

Cited by 63 publications

(30 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Corn stover, a lignocellulosic feedstock, is one of the most important agricultural residues available in high quantities with about 900 million tons produced in 2018 in China according to Ministry of Agriculture and Rural Affairs. Many studies have examined corn stover applied in different fields, such as generating electricity [ 2 ], biofuel [ 3 ] and biochemical production [ 4 ], and biological feed [ 5 ]. Especially, lactic acid (LA) is an important commodity chemical and also a monomer compound to produce biodegradable and biocompatible polylactic acid (PLA), which provides a sustainable alternative to petroleum-derived products [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Zhang

Jian-guo

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Lignocellulose comprised of cellulose and hemicellulose is one of the most abundant renewable feedstocks. Lactic acid bacteria have the ability to ferment sugar derived from lignocellulose. In this study, Pediococcus acidilactici PA204 is a lactic acid bacterium with a high tolerance of temperature and high-efficiency utilization of xylose. We developed a fed-batch simultaneous saccharification and fermentation (SSF) process at 37 °C (pH 6.0) using the 30 FPU (filter paper units)/g cellulase and 20 g/L corn steep powder in a 5 L bioreactor to produce lactic acid (LA). The titer, yield, and productivity of LA produced from 12% (w/w) NaOH-pretreated and washed stover were 92.01 g/L, 0.77 g/g stover, and 1.28 g/L/h, respectively, and those from 15% NaOH-pretreated and washed stover were 104.11 g/L, 0.69 g/g stover, and 1.24 g/L/h, respectively. This study develops a feasible fed-batch SSF process for LA production from corn stover and provides a promising candidate strain for high-titer and -yield lignocellulose-derived LA production.

show abstract

Section: Introductionmentioning

confidence: 99%

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Zhang

Jian-guo

et al. 2020

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Under the conditions of this study, the SSF technology did not produce the predicted effects due to cellulase activity inhibition (product inhibition). The solution to this problem might be the separation of the cellulose saccharification and fermentation processes proposed by Chen et al [20] in their work on the use of corn kernels and corn stalks as raw materials for ethanol production in an integrated technology. At the fermentation stage, the obtained starch and cellulose hydrolysates were combined and completely fermented after 72 h. Similar solutions were proposed for sugar cane, but it was also necessary in this case to carry out costly and time-consuming enzymatic hydrolysis of cellulose in addition to a separate fermentation process [18].…”

Section: Resultsmentioning

confidence: 99%

“…Both technological lines also meet at the fermentation stage, as the preparation of raw materials (grinding or pretreatment) and hydrolysis (mashing with the use of amylolytic enzymes and cellulose hydrolysis with cellulases) is carried out separately. The aim of such a technological line is to obtain a fermentation medium with an increased concentration of fermentable sugars (combination of starch and cellulose hydrolysate) and to reduce the concentration of inhibitors generated during the pretreatment of lignocellulose [20,21].…”

Section: Introductionmentioning

confidence: 99%

Integration of First- and Second-generation Bioethanol Production from Beet molasses and Distillery Stillage After Dilute Sulfuric Acid Pretreatment

Mikulski

Kłosowski

2021

Bioenerg. Res.

View full text Add to dashboard Cite

The possibility of using waste distillery stillage (first-generation technology) after dilute acid pretreatment, as a medium for the preparation of beet molasses mash, for ethanol production according to the simultaneous saccharification and fermentation (SSF) technology, was assessed. The combination of lignocellulosic hydrolysates made from acid-pretreated stillage with sugar-rich beet molasses is an effective way of utilizing the first-generation ethanol production by-products in the second-generation ethanol production technology. It was demonstrated that the final ethanol concentration could be as high as 90 g/L. The process yield was over 94% of the theoretical yield when the molasses was diluted using acid-pretreated maize distillery stillage. An attempt to increase the pool of fermentable sugars by using cellulases to hydrolyze cellulose failed due to product inhibition in the fermentation medium with a high glucose concentration. A more than threefold increase in the concentration of ethyl acetate (even up to 924.4±11.8 mg/L) was observed in the distillates obtained from the media incubated with cellulases. The use of beet molasses combined with the hydrolysate of pretreated distillery stillage also changed the concentration of other volatile by-products. An increase in the concentration of aldehydes (mainly acetaldehyde to a concentration of above 1500 mg/L), methanol, 1-propanol, and 1-butanol was observed, while the concentration of higher alcohols (isobutanol, 2-methyl-1-butanol, 3-methyl-1-butanol) decreased. Interestingly, the use of cellulases in fermentation media from molasses and stillage hydrolysates resulted in an average fourfold increase in the concentration of this ester to a maximum level of 924.4±11.8 mg/L. Hydrolysates made from acid-pretreated distillery stillage, combined with sugar-rich beet molasses to boost the efficiency of the conversion process, can be successfully used in the production of second-generation fuel ethanol. However, further optimization of the cellulose enzymatic hydrolysis process is required for efficient use of the raw material.

show abstract

“…The amounts of sugars (glucose, xylose, cellobiose, and arabinose) of fermentation samples were analyzed by an HPLC system (1200 series; Agilent, Wilmington, DE), which was equipped with a Bio‐Rad Aminex HPX‐87H column and a refractive index detector. The Aminex HPX‐87H column was maintained at 30°C, and the mobile phase was 0.005 M sulfuric acid at 65°C with a flow rate of 0.6 ml/min (S. Chen et al, 2018). All liquid samples were filtered and refrigerated at 4°C until analysis.…”

Section: Methodsmentioning

confidence: 99%

Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

Wen

Ledesma-Amaro

et al. 2020

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

Synthetic microbial communities have become a focus of biotechnological research since they can overcome several of the limitations of single‐specie cultures. A paradigmatic example is Clostridium cellulovorans DSM 743B, which can decompose lignocellulose but cannot produce butanol. Clostridium beijerinckii NCIMB 8052 however, is unable to use lignocellulose but can produce high amounts of butanol from simple sugars. In our previous studies, both organisms were cocultured to produce butanol by consolidated bioprocessing. However, such consolidated bioprocessing implementation strongly depends on pH regulation. Since low pH (pH 4.5–5.5) is required for butanol fermentation, C. cellulovorans cannot grow well and saccharify sufficient lignocellulose to feed both strains at a pH below 6.4. To overcome this bottleneck, this study engineered C. cellulovorans by adaptive laboratory evolution, inactivating cell wall lyases genes (Clocel_0798 and Clocel_2169), and overexpressing agmatine deiminase genes (augA, encoded by Cbei_1922) from C. beijerinckii NCIMB 8052. The generated strain WZQ36: 743B*6.0*3△lyt0798△lyt2169‐(pXY1‐Pthl‐augA) can tolerate a pH of 5.5. Finally, the alcohol aldehyde dehydrogenase gene adhE1 from Clostridium acetobutylicum ATCC 824 was introduced into the strain to enable butanol production at low pH, in coordination with solvent fermentation of C. beijerinckii in consortium. The engineered consortium produced 3.94 g/L butanol without pH control within 83 hr, which is more than 5‐fold of the level achieved by wild consortia under the same conditions. This exploration represents a proof of concept on how to combine metabolic and evolutionary engineering to coordinate coculture of a synthetic microbial community.

show abstract

Integrated bioethanol production from mixtures of corn and corn stover

Cited by 63 publications

References 37 publications

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Integration of First- and Second-generation Bioethanol Production from Beet molasses and Distillery Stillage After Dilute Sulfuric Acid Pretreatment

Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

Contact Info

Product

Resources

About