Integrated bacteria-algal bioreactor for removal of toxic metals in acid mine drainage from iron ore mines

Sahoo, Hrudananda; Senapati, Dilip; Thakur, Indu Sekhar; Naik, Umesh Chandra

doi:10.1016/j.biteb.2020.100422

Cited by 15 publications

(12 citation statements)

References 44 publications

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“…Acid mine drainage (AMD) has a deleterious impact associated with metalliferous mineral exploitation, leaching potentially toxic metals that can affect the environment (Alpers and Nordstorm 1997;Hudson-Edwards et al 2011;Nordstrom 2011;Favas et al 2016;Bogush et al 2016;Rambabu et al 2020;Sahoo et al 2020). Generally, AMD is harmful to the environment nearby and is also thought to affect areas downstream where metals can be transported at some distance (Kumari et al 2010;Ighalo et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of metals by algae from acid mine drainage: a case study of Frongoch Mine (UK)

Bogush

Edwards

et al. 2022

Environ Sci Pollut Res

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In Frongoch Mine (UK), it is unclear the distribution of metals on indigenous algae and whether these species of algae can accumulate metals. This study aimed to investigate the role of indigenous algae for metal removal from acid mine drainage and understand if metals can be adsorbed on the surface of algae or/and bioaccumulated in algae. A sequential extraction procedure was applied for algae samples collected from acid mine drainage (AMD) water to identify the forms in which metals are found in algae. Concentrations of Fe, Pb, Zn, Cu and Cd were evaluated in the algae and AMD samples were collected in June and October 2019. AMDs samples had a pH value ranging between 3.5 and 6.9 and high concentrations of Zn (351 mg/L) and Pb (4.22 mg/L) that exceeded the water quality standards (Water Framework Directive, 2015). Algae Ulothrix sp. and Oedogonium sp. were the two main species in the Frongoch AMDs. The concentrations of metals in algae ranged from 0.007 to 51 mg/g, and the bioconcentration factor of metals decreased in the following order: Fe > > Pb > > Cu > Cd > Zn. It was found that Zn, Cu and Cd were adsorbed onto the surface of and bioaccumulated in the algae, while Pb and Fe were mainly bioaccumulated in the algae. Indigenous algae can be considered as a biogeochemical barrier where metals are accumulating and can be used in bioremediation methods. Also, indigenous algae could be used as a bioindicator to assess water pollution at Frongoch Mine and other similar metal mines.

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Section: Introductionmentioning

confidence: 99%

Bioaccumulation of metals by algae from acid mine drainage: a case study of Frongoch Mine (UK)

Bogush

Edwards

et al. 2022

Environ Sci Pollut Res

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“…In our previous report, a multi-metal tolerant strain Enterobacter sp. AMD01 isolated from an iron-ore mining site, could e ciently able to remove about 95% of toxic metals from acid mine drainage (Sahoo et al 2020). Thus, for proper biological treatment and management of wastewater, there must be the search for tolerant microorganisms and their resistance to toxic environments.…”

Section: Resultsmentioning

confidence: 99%

“…In the present investigation, FTIR was used to identify the changes in functional groups of novel isolated strain and extracted EPS under different stress conditions. Approximately 5 mg of dried biomass and EPS were nely powdered with 50 mg of potassium bromide, powdered samples were pressed and KBr pellets were used for the IR study (Sahoo et al 2020). The infrared spectra (% T) were recorded within a range of 400 to 4000 cm −1 by using a Spectrum RX I Perkin Elmer, FTIR Spectrometer (Thermo scienti c).…”

Section: Antioxidant Assaymentioning

confidence: 99%

“…Exposure of high concentration of chromium has been reported to cause alteration of enzyme speci city, oxidative damage, structural deformation in DNA [DNA-DNA crosslinks, DNA strand breaks, DNA-protein, Cr(III)-DNA adducts, and alkali-labile sites] in biological systems (Bruins et al 2000). Further, metabolic alternation, oxidative damage, morphological disorganization, chlorosis, senescence, abrasion, premature leaf fall, decrease in seed germination index, photosynthetic impairment, stunted vegetative growth, and death of the plant have also been reported due to the chromium toxicity (Mohanty et al 2009;Sahoo et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…There is the possibility of the presence of organic acids and amino acids in the growth medium, which might compete with bacteria for coordination with Cr(III). Thus, IR spectra of the bacterial cell and extracted EPS from both control and Cr(VI) conditions could con rm the involvement of different functional groups for binding of reduced Cr(III)(Mishra et al 2012;Sahoo et al 2020;Das et al 2021). It was observed that the stretch of the hydroxyl group shifted from the range 3710.42 cm -1 (bacterial cells under control condition) to 3724.26 cm -1 (bacterial cells under Cr treated condition); also there was the shift from 3678 cm -1 (EPS from control condition) to 3679.30 cm -1 (EPS from Cr treated condition).Further, some of the common banding patterns have been observed in the range of 3841-3723 cm -1 of both Cr treated EPS and bacterial cells, indicating the interaction of Cr ions to both EPS and bacterial cells as well.…”

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Mechanism of Cr(VI) reduction by an indigenous Rhizobium pusense CR02 isolated from chromite mining quarry water (CMQW) at Sukinda Valley, India

Sahoo

Kisku

Varadwaj

et al. 2022

Environ Sci Pollut Res

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Toxicological assessment of CMQW generated due to chromite mining activities at Sukinda, valley has revealed high chromium contamination along with Zn and Fe. The present study focused on mechanism of chromate reduction by an indigenous multi-metal tolerant bacterium Rhizobium pusense CR02 isolated from CMQW. Isolated strain has shown resistance upto (520 mg/L) of Cr(VI) with IC50 value of 385.4 mg/L. The highest reduction rate 8.6×10 -2 /h was recorded with 20 mg/L of initial concentration of Cr(VI). Extracellular (3.06±0.012 U/mL), intracellular (3.60±0.13 U/mL) and membrane (1.89±0.01 U/mL) associated chromate reductases were found to be involved for reduction. The extracellular polymeric substances (EPS) produced by the isolate also enhanced reduction activity of 46.32±1.69 mg/L after 72 h with initial concentration of 50 mg/L. FTIR analysis revealed the involvement of functional groups -OH, -CO and -NH for Cr(VI) biosorption whereas P=O, -CO-NH-and -COOH interacted with Cr(III). Zeta potential with less negative surface charge favored reduction of Cr(VI). Treatment of CMQW by bacterial isolate detoxi ed Cr(VI) minimizing chromosomal aberrations in root cells of Allium cepa L., suggesting the role of Rhizobium pusense CR02 as a promising bio-agent for Cr(VI) detoxi cation. DeclarationsCredit author statement Hrudananda Sahoo: Conceptualization, methodology, execution of all experiments, data analysis, validation, manuscript writing, review and editing. Kanika Kisku, Kumar S. K. Varadwaj, and Prasannajit Acharya: Data analysis, review, and editing. Umesh Chandra Naik: Conceptualization, validation of data, review, and editing.Acknowledgments would like to express our sincere thanks to SERB, Govt. of India (YSS/2015/001089) for the nancial support. We would also acknowledge University Grants Commission (UGC), Govt. of India for providing Fellowship. We would also like to acknowledge CIF, OUAT, Bhubaneswar for ICP-OES and Zeta potential analysis; CSIR-NIIST, Thiruvananthapuram, Kerala for XPS analysis, and ILS, Bhubaneswar for DNA sequencing facilities. We are also highly thankful to the research

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Characterization of multi-metal-resistant Serratia sp. GP01 for treatment of effluent from fertilizer industries

2021

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The e uent generated from fertilizer plants in Paradeep in the coast of the Bay of Bengal is the major pollutant causing health hazard in the vicinity of the area with respect to plants, animals and microbes.Samples of e uent were found to contain heavy metals (mg L -1 ): Cr (100), Ni (36.975), Mn (68.673), Pb (20.133), Cu (74.44), Zn (176.716), Hg (5.358) and As (24.287) as analyzed by XRF. Indigenous bacterial strains were screened for chromate and multi-metal resistance to remediate the toxic pollutants. The isolated strain G1 was identi ed as Serratia sp. through 16S-rDNA sequence homology. Potent strain Serratia sp. GP01 treated with 100 mg L -1 of K 2 Cr 2 O 7 has shown the e cacy of reducing 69.05 mg L -1 of Cr over 48 h of incubation. Further, presence of chromate reductase gene (ChR) in Serratia sp. con rmed the enzymatic reduction of Cr (VI). SEM-EDX and SEM mapping analysis revealed substantial biosorption of Cr and other heavy metals present in e uent by Serratia sp. GP01. Antioxidant enzymes such as catalase (72.15 U mL -1 ), SOD (57.14 U mL -1 ) and peroxidase (62.49 U mL -1 ) were found to be higher as compared to the control condition. FTIR study also revealed the role of N-H, O-H, C = C, C-H, C-O, C-N, and C = O functional groups of the cell surface of Serratia sp. treated with K 2 Cr 2 O 7 and e uent from the fertilizer industry. Isolated strain Serratia sp. could be used for the detoxi cation of Cr (VI) and other heavy metals in fertilizer plant e uent.

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Integrated bacteria-algal bioreactor for removal of toxic metals in acid mine drainage from iron ore mines

Cited by 15 publications

References 44 publications

Bioaccumulation of metals by algae from acid mine drainage: a case study of Frongoch Mine (UK)

Bioaccumulation of metals by algae from acid mine drainage: a case study of Frongoch Mine (UK)

Mechanism of Cr(VI) reduction by an indigenous Rhizobium pusense CR02 isolated from chromite mining quarry water (CMQW) at Sukinda Valley, India

Characterization of multi-metal-resistant Serratia sp. GP01 for treatment of effluent from fertilizer industries

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