Integrated analysis of promoter methylation and expression of telomere related genes in breast cancer

Heng, Jianfu; Zhang, Fan; Guo, Xinwu; Tang, Lili; Peng, Limin; Luo, Xipeng; Xu, Xunxun; Wang, Shouman; Dai, Linjie; Wang, Jun

doi:10.18632/oncotarget.16036

Cited by 19 publications

(11 citation statements)

References 61 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tel1 promotes MRX recruitment, whereas Rif2 acts as a negative regulator by enhancing ATP hydrolysis that opens Rad50, facilitating MRX release from DNA (184). In line with insights from yeast experiments, changes in expression and posttranslational modification of MRN and telomeric proteins are associated with various diseases in humans (185–187). In mouse embryonic fibro-blasts, the MRN complex is required for the response to telomere dysfunction, consistent with the idea that initiating this response shares common elements with the response to interstitial DNA damage (188).…”

Section: Mrn and Dysfunctional Telomeresmentioning

confidence: 82%

The MRE11–RAD50–NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair

Syed

Tainer

2018

Annu. Rev. Biochem.

323

345

View full text Add to dashboard Cite

Genomic instability in disease and its fidelity in health depend on the DNA damage response (DDR), regulated in part from the complex of meiotic recombination 11 homolog 1 (MRE11), ATP-binding cassette–ATPase (RAD50), and phosphopeptide-binding Nijmegen breakage syndrome protein 1 (NBS1). The MRE11–RAD50–NBS1 (MRN) complex forms a multifunctional DDR machine. Within its network assemblies, MRN is the core conductor for the initial and sustained responses to DNA double-strand breaks, stalled replication forks, dysfunctional telomeres, and viral DNA infection. MRN can interfere with cancer therapy and is an attractive target for precision medicine. Its conformations change the paradigm whereby kinases initiate damage sensing. Delineated results reveal kinase activation, posttranslational targeting, functional scaffolding, conformations storing binding energy and en-abling access, interactions with hub proteins such as replication protein A (RPA), and distinct networks at DNA breaks and forks. MRN biochemistry provides prototypic insights into how it initiates, implements, and regulates multifunctional responses to genomic stress.

show abstract

Section: Mrn and Dysfunctional Telomeresmentioning

confidence: 82%

The MRE11–RAD50–NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair

Syed

Tainer

2018

Annu. Rev. Biochem.

323

345

View full text Add to dashboard Cite

show abstract

“…The on-chip bisulfite conversion was processed in a microfluidic chip which was integrated with the MS-PCR analysis unit, the device has achieved the methylated DNA detection within 80 min with low-input sample (Yoon et al, 2015). These microfluidic chips with capabilities of bisulfite conversion can not only be integrated with self-made testing equipments Heng et al, 2017), but also can be used in combination with commercial testing equipment, for example, the bisulfite conversion of DNA by a microfluidic chip was reported to be subjected to Fluidigm, which successfully accessed multiple CpG loci in multiple DNA samples with high reproducibility (Adamowicz et al, 2018). Researchers also try to use the analysis of DNA melting temperature to determine the methylation of one or more DNA regions.…”

Section: Microfluidic Technology To Study Breast Cancer Epigenomicsmentioning

confidence: 99%

Perspectives on the Role of Histone Modification in Breast Cancer Progression and the Advanced Technological Tools to Study Epigenetic Determinants of Metastasis

et al. 2020

View full text Add to dashboard Cite

Metastasis is a complex process that involved in various genetic and epigenetic alterations during the progression of breast cancer. Recent evidences have indicated that the mutation in the genome sequence may not be the key factor for increasing metastatic potential. Epigenetic changes were revealed to be important for metastatic phenotypes transition with the development in understanding the epigenetic basis of breast cancer. Herein, we aim to present the potential epigenetic drivers that induce dysregulation of genes related to breast tumor growth and metastasis, with a particular focus on histone modification including histone acetylation and methylation. The pervasive role of major histone modification enzymes in cancer metastasis such as histone acetyltransferases (HAT), histone deacetylases (HDACs), DNA methyltransferases (DNMTs), and so on are demonstrated and further discussed. In addition, we summarize the recent advances of next-generation sequencing technologies and microfluidic-based devices for enhancing the study of epigenomic landscapes of breast cancer. This feature also introduces several important biotechnologists for identifying robust epigenetic biomarkers and enabling the translation of epigenetic analyses to the clinic. In summary, a comprehensive understanding of epigenetic determinants in metastasis will offer new insights of breast cancer progression and can be achieved in the near future with the development of innovative epigenomic mapping tools.

show abstract

“…Previous publications indicate the deregulated expression of TERF1 in cancer [33][34][35]. In order to evaluate the potential contribution of the TERF1-tsi to testis cancer, we analyzed its expression by RT-PCR and IHC.…”

Section: Terf1-tsi Expression Is Downregulated In Seminomasmentioning

confidence: 99%

A Novel Tissue and Stem Cell Specific TERF1 Splice Variant Is Downregulated in Tumour Cells

Morcos

Najjar

Meessen

et al. 2019

IJMS

View full text Add to dashboard Cite

In this study, we describe the identification of a novel splice variant of TERF1/PIN2, one of the main components of the telomeric shelterin complex. This new splice variant is identical to TERF1, apart from a 30 amino acid internal insertion near to the C-terminus of TERF1. Based on genome comparison analyses and RNA expression data, we show that this splice variant is conserved among hominidae but absent from all other species. RNA expression and histological analyses show specific expression in human spermatogonial and hematopoietic stem cells (HSCs), while all other analyzed tissues lack the expression of this TERF1-isoform, hence the name TERF1-tsi (TERF1-tissue-specific-isoform). In addition, we could not detect any expression in primary human cells and established cancer cell lines. Immunohistochemistry results involving two new rabbit polyclonal antibodies, generated against TERF1-tsi specific peptides, indicate nuclear localization of TERF1-tsi in a subset of spermatogonial stem cells. In line with this observation, immunofluorescence analyzes in various cell lines consistently revealed that ectopic TERF1-tsi localizes to the cell nucleus, mainly but not exclusively at telomeres. In a first attempt to evaluate the impact of TERF1-tsi in the testis, we have tested its expression in normal testis samples versus matched tumor samples from the same patients. Both RT-PCR and IHC show a specific downregulation of TERF1-tsi in tumor samples while the expression of TERF1 and PIN2 remains unchanged.

show abstract

Integrated analysis of promoter methylation and expression of telomere related genes in breast cancer

Cited by 19 publications

References 61 publications

The MRE11–RAD50–NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair

The MRE11–RAD50–NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair

Perspectives on the Role of Histone Modification in Breast Cancer Progression and the Advanced Technological Tools to Study Epigenetic Determinants of Metastasis

A Novel Tissue and Stem Cell Specific TERF1 Splice Variant Is Downregulated in Tumour Cells

Contact Info

Product

Resources

About