Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation

Wang, Jun; Yan, Xiaoxiao; Nesengani, Lucky T.; Yang, Lei; Lu, Wenjing

doi:10.1016/j.micpath.2017.11.053

Cited by 9 publications

(5 citation statements)

References 42 publications

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“…As a naturally formed posttranscriptional regulatory factor, miRNAs are the regulatory factors that regulate gene expression at posttranscription level; besides, miRNAs have a series of extensive target mRNAs, and over 50% miRNAs are located in tumor-related genomic domains and fragile sites [ 7 ], implying that the disordered miRNA expression will exert a vital role in the pathogenesis of proliferative disease. Therefore, changing miRNA expression can lead to abnormal expression of many genes and gene products, thus significantly affecting cell viability.…”

Section: Introductionmentioning

confidence: 99%

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Nai

Zhang

et al. 2022

BioMed Research International

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EMs is a kind of benign disease with certain malignant behaviors. The adhesion, invasive growth, and angiogenesis of ectopic endometrial cells are the pathological basis of EMs occurrence, but its etiology and pathogenesis have not been completely illustrated yet. In our research, we aim to investigate the role of miR-363 in the pathogenesis of endometriosis. Real-time quantitative PCR was used to detect the expression of miR-363 before and after ESC/NSC transfection. CCK-8, flow cytometry, and transwell assay were used to detect the effect of the miR-363 expression on cell proliferation, apoptosis, and invasion. The effects of the miR-363 expression on the contents of Fas/APO-1 and ICAM-1 in cell culture supernatant were detected by ELISA. qRT-PCR and WB assay were used to detect the effects of the miR-363 expression on the mRNA and protein expression levels of ICAM-1, MMP-7, and VEGF in ESC. The increased expression of miR-363 could inhibit the proliferation and invasion of ESC, promote apoptosis, and inhibit the secretion of FAS/APO-1 and ICAM-1. The knockdown expression of miR-363 promoted proliferation and invasion of NSC, inhibited apoptosis, and promoted secretion of FAS/APO-1 and ICAM-1. VCAM-1, VEGF, and MMP-7 were detected in ESCs before transfection. The protein expression level was higher than that of NSCs. Compared with pretransfection, the protein levels of VCAM-1, VEGF, and MMP-7 in the M-363 group were significantly downregulated. The downregulated expression of miR-363 was associated with a stronger cell proliferation ability, a lower cell apoptosis rate, and a stronger ESC. Migration is associated with invasiveness, proliferation, angiogenesis, and immune escape. The low expression of miR-363 promotes endogenesis through posttranscriptional regulation of target genes VCAM-1, MMP-7, and VEGF. The differential expression of miR-363 between ESC and NSC may be an important factor in the many biological differences between ESC and NSC.

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Section: Introductionmentioning

confidence: 99%

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Nai

Zhang

et al. 2022

BioMed Research International

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“…The mapped small RNA tags were used to search for known miRNAs in the miRNA database, miRNABase 20.0 [ 47 ]. With the use of miRNABase 20.0 as the reference database, modified software miRNADeep2 [ 48 ] and srna-tools-cl were used to predict the pre-miRNAs and draw the secondary structures [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

Sun

Long

et al. 2022

IJMS

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A lobed leaf is a common trait in plants, but it is very rare in Lauraceae plants, including species of Phoebe. In the study of germplasm resources of Phoebe neurantha, we found lobed leaf variant seedlings, and the variation could be inherited stably. Studying the lobed leaf mechanism of P. neurantha var. lobophylla can offer insight into the leaf development mechanism of woody plants. RNA-seq and small RNA-seq analysis results showed that a total of 8091 differentially expressed genes (DEGs) and 16 differentially expressed miRNAs were identified in P. neurantha var. lobophylla. Considering previous research results, a leaf margin morphological development related miRNA, pne-miRNA319a, was primary identified as a candidate miRNA. Target gene prediction showed that a total of 2070 genes were predicted to be the target genes of differentially expressed miRNAs. GO enrichment analysis of differentially expressed target genes suggested that PnTCP2 is related to lobed leaf formation. The TRV-VIGS gene silencing of PnTCP2 led to lobed leaves in P. neurantha seedlings. The downregulation of PnTCP2 led to lobed leaves. The yeast two-hybrid test and bimolecular fluorescence complementation test confirmed that the PnTCP2 protein interacted with the PnLBD41 protein. Based on the expression analysis of gene-silenced leaves and RNA-seq and small RNA-seq analysis results, pne- miRNA319a and PnLBD41 might also play important roles in this process. In conclusion, PnTCP2 plays an important and vital role in the formation of the lobed leaves of P. neurantha var. lobophylla.

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“…Genome-wide characterization is crucial for understanding the development and pathophysiology of bovine endometritis, Abbreviations: BESC, bovine endometrial stromal cells; BEEC, bovine endometrial epithelial cells; LPS, lipopolysaccharide; DEGs, differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; PPARs, peroxisome proliferator-activated receptors; RNA-Seq, high-throughput mRNA sequencing; PCA, principal component analysis; LBP, lipoprotein binding protein; PRRs, innate pattern recognition receptors; AMP, antimicrobial peptides; LAP, lingual antimicrobial peptide; TAP, tracheal antimicrobial peptide; MD-2, myeloid differentiation factor 2; FDR, false discovery rate; IRF3, interferon regulatory factor 3; MAP kinase, mitogen-activated protein kinase; ISG, IFN-stimulated genes; GAPDH, glyceraldehyde 3-phosphatedehydrogenase; STEAP4, six-transmembrane epithelial antigen of prostate 4; CAM, cell adhesion molecules; VCAM-1, vascular cell adhesion molecule-1; HA, hyaluronan; ECM, extracellular matrix; HAS2, hyaluronan synthase 2. which will be beneficial for prevention and treatment of endometritis. Previous studies on RNA-seq analysis in BESCintegrated BEECs treated with LPS showed 20 differentially expressed miRNAs and 108 differentially expressed genes (DEGS) and enriched 118 Gene Ontology (GO) and 66 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which provided an understanding on the effect of miRNA on bovine endometritis (21). Another study on RNA-seq analysis of the change in miRNA and mRNA expressions in 15 cows at 7 and 21 days postpartum showed 4,197 DEGs in healthy cows, while only 31 DEGs in cows with cytological endometritis from proinflammatory to hyperplasia stages (22).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Transcriptomic Changes in Bovine Endometrial Stromal Cells Treated With Lipopolysaccharide

Ding

Deng

et al. 2020

Front. Vet. Sci.

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Endometritis adversely affects the ability of cattle to reproduce and significantly reduces milk production. The is mainly composed of epithelial and stromal cells, and they produce the first immune response to invading pathogens. However, most of the epithelial cells are disrupted, and stromal cells are exposed to an inflammatory environment when endometritis occurs, especially postpartum. Many bacteria and toxins start attacking stromal cell due to loss of epithelium, which stimulates Toll-like receptor (TLRs) on stromal cells and causes upregulated expression of cytokines. Understanding the genome-wide characterization of bovine endometritis will be beneficial for prevention and treatment of endometritis. In this study, whole-transcriptomic gene changes in bovine endometrial stromal cells (BESCs) treated with LPS were compared with those treated with PBS (control group) and were analyzed by RNA sequencing. Compared with the control group, a total of 366 differentially expressed genes (DEGs) were identified in the LPS-induced group (234 upregulated and 132 downregulated genes), with an adjusted P < 0.05 by DESeq. Gene Ontology (GO) enrichment analysis revealed that DEGs were most enriched in interleukin-1 receptor binding, regulation of cell activation, and lymphocyte-activated interleukin-12 production. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed DEGs were most enriched in the TNF signaling pathway, Toll-like receptor signaling pathway, cytokine–cytokine receptor interaction, NF-κB signaling pathway, and chemokine signaling pathway. The results of this study unraveled BESCs affected with LPS transcriptome profile alterations, which may have a significant effect on treatment inflammation by comprehending molecular mechanisms and authenticating unique genes related to endometritis.

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Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation

Cited by 9 publications

References 42 publications

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

Effects of miR-363 on the Biological Activities of Eutopic Endometrial Stromal Cells in Endometriosis

The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

Analysis of Transcriptomic Changes in Bovine Endometrial Stromal Cells Treated With Lipopolysaccharide

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