Integrated analysis identifies a pathway-related competing endogenous RNA network in the progression of pancreatic cancer

Abstract

Background It is well acknowledged that cancer-related pathways play pivotal roles in the progression of pancreatic cancer (PC). Employing Integrated analysis, we aim to identify the pathway-related ceRNA network associated with PC progression. Methods We divided eight GEO datasets into three groups according to their platform, and combined TCGA and GTEx databases as a group. Additionally, we screened out the differentially expressed genes (DEGs) and performed functional enrichment analysis in each group, and recognized the top hub genes in the most enriched pathway. Furthermore, the upstream of miRNAs and lncRNAs were predicted and validated according to their expression and prognostic roles. Finally, the co-expression analysis was applied to identify a pathway-related ceRNA network in the progression of PC. Results A total of 51 significant pathways that common enriched in all groups were spotted. Enrichment analysis indicated that pathway in cancer was greatly linked with tumor formation and progression. Next, the top 20 hug genes in this pathway were recognized, and stepwise prediction and validation from mRNA to lncRNA, including 11 hub genes, 4 key miRNAs, and 2 key lncRNAs, were applied to identify a meaningful ceRNA network according to ceRNA rules. Ultimately, we identified the PVT1/miR-20b/CCND1 axis as a promising pathway-related ceRNA axis in the progression of PC. Conclusion Overall, we elucidate the pathway-related ceRNA regulatory network of PVT1/miR-20b/CCND1 in the progression of PC, which can be considered as therapeutic targets and encouraging prognostic biomarkers for PC.

MMP25-AS1/hsa-miR-10a-5p/SERPINE1 axis as a novel prognostic biomarker associated with immune cell infiltration in KIRC

“…A large number of studies have found that long non-coding RNAs (lncRNAs) can act as miRNA sponges to regulate target gene expression. 7 , 8 The lncRNA-miRNA-mRNA ceRNA network has been shown to play vital roles in progression and metastasis of multiple cancers, such as colorectal cancer, 9 pancreatic cancer, 10 , 11 liver cancer, as well as breast cancer. 12 , 13 , 14 Nevertheless, the pivotal lncRNA-miRNA-mRNA ceRNA networks that are significantly associated with prognosis of KIRC still need further research.…”

Long noncoding RNA DLGAP1-AS1 promotes the progression of glioma by regulating the miR-1297/EZH2 axis

“…Several studies have reported the close association of aberrant lncRNAs and miRNAs expressions to tumor development, including glioma [ 24 , 25 ]. lncRNA HAS2-AS1 promoted tumour progression in glioma by acting as a ceRNA [ 26 ].…”

“…HCG11 modulated glioma progression through its action on the miR-496/CPEB3 axis [ 27 ]. Moreover, DLGAP1-AS1 has also been implicated in several processes involved in tumor progression [ 24 , 25 ]. However, the expression and functional role of DLGAP1-AS1 in glioma has yet to be completely characterized.…”

Long Noncoding Competing Endogenous RNA Networks in Pancreatic Cancer

“…By searching The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases and performing functional enrichment analysis, Zu et al. ( 274 ) recognized that pathways in cancer was greatly associated with tumor formation and progression. To identify a meaningful ceRNA network, the stepwise prediction and validation from mRNA to lncRNA were applied according to the ceRNA rules.…”