Integrated 16S rDNA Gene Sequencing and Untargeted Metabolomics Analyses to Investigate the Gut Microbial Composition and Plasma Metabolic Phenotype in Calves With Dampness-Heat Diarrhea

Yan, Zunxiang; Zhang, Kang; Zhang, Kai; Wang, Guibo; Wang, Lei; Zhang, Jingyan; Qiu, Zhengying; Guo, Zhuang; Song, Xiaoping; Li, Jianxi

doi:10.3389/fvets.2022.703051

Cited by 13 publications

(15 citation statements)

References 73 publications

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“…Diarrhoea alters the composition of the intestinal microbiome of calves. Research (Yan et al, 2022) found changes in 14 genera, which included Bacteroides, Shigella, Fournierella, Escherichia.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Therapeutic Effect of the Use of Bacillus Coagulans in Calf Dyspepsia

Шкромада¹,

Фотіна²,

BEREZOVSKYI³

et al. 2022

Scientific Horizons

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The relevance of this study lies in the use of the probiotic strain of the microorganism B. coagulans ALM 86 for the treatment of diarrhoea in dairy calves and the reduction of the use of antibiotics in animal husbandry. The purpose of this paper was to establish the therapeutic effect in the treatment of diarrhoea in calves, the composition of the microflora, physiological indicators and metabolism using Bacillus coagulans ALM 86. The study used the following methods: microbiological, microscopic, biochemical, physiological, statistical. Studies have shown that the use of B. coagulans ALM 86 contributes to a decrease in the number of opportunistic microflora E. coli – by 90.8%, S. aureus – by 92.4%, S. enterica – by 88.01, an increase in Lactobacillus sp. by 130.8–151.58% and Bifidobacterium sp. by 272.7–469.8%. Administration of B. coagulans ALM 86 at a concentration of 1×109, CFU/g at a dose of 3–5 g helped stop diarrhoea and accelerate the development of scar digestion for four days in dairy calves, compared to the group where the antibiotic was used. An increase in average daily growth and feed consumption was found in the first experimental group by 19.7–15.0%, in the second – by 23.4–19.9% (р≤0.05). A decrease in the level of total cholesterol with the use of B. coagulans ALM 86, an increase in the level of total protein by 18.57–22.6%, as a result of an increase in the content of total globulins by 49.3–57.37%, respectively (р≤0.05), were proven. Administration of B. coagulans ALM 86 at a concentration of 1×109, CFU/g at a dose of 3–5 g helped stop diarrhoea and accelerate the development of scar digestion for four days in dairy calves, compared to the group where the antibiotic was used. The obtained results of the study indicate the absence of toxic effects of the proposed dose of B. coagulans ALM 86 for the treatment of dyspepsia in calves and obtaining a positive effect. The practical value of the obtained results lies in the possibility of using B. coagulans ALM 86 in the treatment of diarrhoea in dairy calves without the use of antibiotics on animal farms of various designation.

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Section: Introductionmentioning

confidence: 99%

Determination of the Therapeutic Effect of the Use of Bacillus Coagulans in Calf Dyspepsia

Шкромада¹,

Фотіна²,

BEREZOVSKYI³

et al. 2022

Scientific Horizons

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“…In this experiment, the modeling methods and the results of symptoms in the model mice were consistent with the diagnostic criteria of DSH, indicating that the model was successfully replicated ( Hui et al., 2021 ). The main clinical sign of diarrhea with DSH is closely related to gut microbiota dysbiosis ( Gong et al., 2015 ; Yan et al., 2022 ). Studies have shown that digestive tract diseases occur primarily due to structural changes in bacteria of typical origin ( Sorbara and Pamer, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Alteration of intestinal mucosal microbiota in mice with Chinese dampness-heat syndrom diarrhea by improper diet combined with high temperature and humidity environments

Qiao

Peng

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundEnvironment, diet, and emotion may trigger diarrhea, but the mechanism is unclear. Dietary habits or environmental factors affect the composition of gut microbiota. This study aimed to investigate the effects of improper diet combined with high humidity and temperature (HTH) environment on the intestinal mucosal microbiota.Materials and methodsKunming mice were randomly assigned to two equal groups of five mice, namely the control (ccm) group and the model (cmm) group. Diarrhea mice with dampness-heat (DSH) were established by improper diet combined with HTH environments. We used 16S rRNA gene amplicon sequencing to analyze the characteristics of intestinal mucosal microbiota and the interaction relationship of function.ResultsOur study shows that the intestinal mucosal microbiota of mice changed significantly after an improper diet combined with the HTH environments. The abundance of Fusobacteria and Haemophilus increased dramatically in the cmm group compared to the ccm group (P<0.05). And the abundance of Firmicutes, Lactobacillus, and Lonsdalea was significantly decreased in the cmm group (P<0.05). According to the functional predictive analysis, we found that Lactobacillus showed a significant negative correlation with Protein export, Homologous recombination, Phenylalanine, tyrosine, tryptophan biosynthesis, Citrate cycle, and Lipoic acid metabolism.ConclusionDiarrhea with DSH constructed under improper diet and HTH environment may be related to Lactobacillus and Haemophilus. And long-term consumption of improper diet and the HTH environment may affect metabolism.

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“…The calves were fed colostrum at a dose of 4 kg/calf only on the first day of postpartum, and then fed the pasteurized normal milk at a dose of 6 kg/day, and the calf feed was fed from the third day of postpartum, and the feeding and drink tools were washed and disinfected two times daily. The diagnosis of calves with DHD followed the protocol in our previous study ( 12 ), which means that the DHD case was mainly characteristic with diarrhea as well as the symptom of red tongue coated with thick greasy tongue-fur or the symptom of feces with mucus, blood, or purulence. At the same time, the DHD case must show two out of total minor symptoms in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Recently, metabolomics was used to study the mechanism of diarrhea in calves, and acetylcarnitine, indoxyl sulfate, and oxindole have been recognized to be potential biomarkers for calf diarrhea ( 11 ). Additionally, metabolites such as lecithin and choline have been found to affect the progression of DHD by interfering with the metabolism of arachidonic acid, linoleic acid, and glycerophospholipids in calves ( 12 ). Previous studies on the structure and function of fecal microflora of calves with DHD indicated that DHD had a significant effect on the intestinal microbial compositions in calves, including significant changes in Escherichia-Shigella, Bacteroides, and Fournierella in sick calves ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, metabolites such as lecithin and choline have been found to affect the progression of DHD by interfering with the metabolism of arachidonic acid, linoleic acid, and glycerophospholipids in calves ( 12 ). Previous studies on the structure and function of fecal microflora of calves with DHD indicated that DHD had a significant effect on the intestinal microbial compositions in calves, including significant changes in Escherichia-Shigella, Bacteroides, and Fournierella in sick calves ( 12 ). These studies imply that systems biology makes it possible to screen potential pathological targets for calves with DHD.…”

Section: Introductionmentioning

confidence: 99%

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Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves

Yan

Zhang²,

Wang³

et al. 2022

Front. Vet. Sci.

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Dampness heat diarrhea (DHD) is one of the most common syndromes of calf diarrhea. Its complex etiology and lack of objective diagnostic criteria bring great challenges to the diagnosis and treatment of this disease. This study aims to screen some prospective diagnostic biomarkers or therapeutic targets for calves with DHD by investigating the differential protein profiles of plasma between DHD calves and clinically healthy calves by mass spectrometry-based proteomic. A total of 120 DHD calves and 90 clinically healthy calves were divided into two groups randomly, 30 DHD calves and 30 clinically healthy calves in the test group, and 90 DHD calves and 60 clinically healthy calves in the validation group. In the test group, a total of 52 proteins were differentially expressed between calves with DHD and clinically healthy calves, 13 proteins were significantly increased and 39 proteins were significantly decreased. The differentially expressed proteins were associated with the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway. In the validation group, 13 proteins were selected from 52 differential expression proteins for parallel reaction monitoring validation to verify their associations with DHD calves. The targeted proteomic results showed that fibronectin precursor (FN1) and apolipoprotein C-IV precursor (APOC4) were significantly associated with DHD in calves, and they were downregulated in sick calves. In conclusion, the differential expression of plasma proteins was associated with DHD pathogenesis in calves, and the FN1 and APOC4 might be the potential clinical biomarkers for diagnosis of DHD in calves, and the intestinal immune network of IgA production, caffeine metabolism, purine metabolism, and PI3K signaling pathway are the candidate targets to treat DHD in calves. Our finding provides a reference for further investigating the pathogenesis, developing techniques of diagnosis, and screening treatment drugs for DHD in calves.

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Integrated 16S rDNA Gene Sequencing and Untargeted Metabolomics Analyses to Investigate the Gut Microbial Composition and Plasma Metabolic Phenotype in Calves With Dampness-Heat Diarrhea

Cited by 13 publications

References 73 publications

Determination of the Therapeutic Effect of the Use of Bacillus Coagulans in Calf Dyspepsia

Determination of the Therapeutic Effect of the Use of Bacillus Coagulans in Calf Dyspepsia

Alteration of intestinal mucosal microbiota in mice with Chinese dampness-heat syndrom diarrhea by improper diet combined with high temperature and humidity environments

Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves

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