Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves

Yan, Zunxiang; Zhang, Kang; Wang, Guibo; Wang, Lei; Zhang, Jingyan; Qiu, Zhengying; Guo, Zhuang; Zhang, Kai; Li, Jianxi

doi:10.3389/fvets.2022.986329

Cited by 2 publications

(2 citation statements)

References 61 publications

(64 reference statements)

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“…However, when it comes to investigating the development of diarrhoea in animals, plasma emerges as an optimal choice for sampling. This preference arises from its comparatively stable chemical and physical properties, rich protein content, and relatively reliable collection techniques [ 22 ]. We can add to these facts that since haemorrhagic diarrhoea affects the overall physiological functioning of the body, plasma protein profiling offers valuable insights into these perturbations, making it a very suitable material for diagnosis and biomarker discovery.…”

Section: Resultsmentioning

confidence: 99%

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

Huber,

Kuropka,

Doulidis

et al. 2024

PLoS ONE

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Acute haemorrhagic diarrhoea is a common complaint in dogs. In addition to causes like intestinal parasites, dietary indiscretion, intestinal foreign bodies, canine parvovirus infection, or hypoadrenocorticism, acute haemorrhagic diarrhoea syndrome (AHDS) is an important and sometimes life-threatening differential diagnosis. There is some evidence supporting the link between Clostridium perfringens toxins and AHDS. These toxins may be partially responsible for the epithelial cell injury, but the pathogenesis of AHDS is still not fully understood. Recent studies have suggested that severe damage to the intestinal mucosa and associated barrier dysfunction can trigger chronic gastrointestinal illnesses. Besides bloodwork and classical markers for AHDS such as protein loss and intestinal bacterial dysbiosis, we focused mainly on the plasma-proteome to identify systemic pathological alterations during this disease and searched for potential biomarkers to improve the diagnosis. To accomplish the goals, we used liquid chromatography-mass spectrometry. We compared the proteomic profiles of 20 dogs with AHDS to 20 age-, breed-, and sex-matched control dogs. All dogs were examined, and several blood work parameters were determined and compared, including plasma biochemistry and cell counts. We identified and quantified (relative quantification) 207 plasmatic proteins, from which dozens showed significantly altered levels in AHDS. Serpina3, Lipopolysaccharide-binding protein, several Ig-like domain-containing proteins, Glyceraldehyde-3-phosphate dehydrogenase and Serum amyloid A were more abundant in plasma from AHDS affected dogs. In contrast, other proteins such as Paraoxonase, Selenoprotein, Amine oxidases, and Apolipoprotein C-IV were significantly less abundant. Many of the identified and quantified proteins are known to be associated with inflammation. Other proteins like Serpina3 and RPLP1 have a relevant role in oncogenesis. Some proteins and their roles have not yet been described in dogs with diarrhoea. Our study opens new avenues that could contribute to the understanding of the aetiology and pathophysiology of AHDS.

show abstract

Section: Resultsmentioning

confidence: 99%

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

Huber,

Kuropka,

Doulidis

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…However, when it comes to investigating the development of diarrhoea in animals, plasma emerges as an optimal choice for sampling. This preference arises from its comparatively stable chemical and physical properties, rich protein content, and relatively reliable collection techniques [24]. We can add to these facts that since haemorrhagic diarrhoea affects the overall physiological functioning of the body, plasma protein profiling offers valuable insights into these perturbations, making it a very suitable material for diagnosis and biomarker discovery.…”

Section: Discussionmentioning

confidence: 99%

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

Huber,

Kuropka,

Doulidis

et al. 2023

Preprint

View full text Add to dashboard Cite

Acute haemorrhagic diarrhoea is a common complaint in dogs. In addition to causes like intestinal parasites, dietary indiscretion, intestinal foreign bodies, canine parvovirus infection, or hypoadrenocorticism, acute haemorrhagic diarrhoea syndrome (AHDS) is an important and sometimes life-threatening differential diagnosis. There is some evidence supporting the link betweenClostridium perfringenstoxins and AHDS. These toxins may be partially responsible for the epithelial cell injury, but the pathogenesis of AHDS is still not fully understood. Recent studies have suggested that severe damage to the intestinal mucosa and associated barrier dysfunction can trigger chronic gastrointestinal illnesses. Besides bloodwork and classical markers for AHDS such as protein loss and intestinal bacterial dysbiosis, we focused mainly on the plasma-proteome to identify systemic pathological alterations during this disease and searched for potential biomarkers to improve the diagnosis. To accomplish the goals, we used liquid chromatography-mass spectrometry. We compared the proteomic profiles of 20 dogs with AHDS to 20 age-, breed-, and sex-matched control dogs. All dogs were examined, and several blood work parameters were determined and compared, including plasma biochemistry and cell counts. We identified and quantified 207 plasmatic proteins, from which dozens showed significantly altered levels in AHDS. Serpina3, Lipopolysaccharide-binding protein, several Ig-like domain-containing proteins, Glyceraldehyde-3-phosphate dehydrogenase and Serum amyloid A were more abundant in plasma from AHDS affected dogs. In contrast, other proteins such as Paraoxonase, Selenoprotein, Amine oxidases, and Apolipoprotein C-IV were significantly less abundant. Many of the identified and quantified proteins are known to be associated with inflammation. Other proteins like Serpina3 and RPLP1 have a relevant role in oncogenesis. Some proteins and their roles have not yet been described in dogs with diarrhoea. Our study opens new avenues that could contribute to the understanding of the aetiology and pathophysiology of AHDS.

show abstract

Differential proteomic of plasma provides a new perspective on scientific diagnosis and drug screening for dampness heat diarrhea in calves

Cited by 2 publications

References 61 publications

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)

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