Insulin Regulates Adipocyte Lipolysis via an Akt-Independent Signaling Pathway

Choi, Sarah M.; Tucker, David; Gross, Danielle N.; Easton, Rachael; DiPilato, Lisa M.; Dean, Abigail S.; Monks, Bob; Birnbaum, Morris J.

doi:10.1128/mcb.00797-10

Cited by 182 publications

(151 citation statements)

References 73 publications

(85 reference statements)

Supporting

Mentioning

135

Contrasting

Unclassified

Order By: Relevance

“…Insulin can also stimulate fatty acid synthase (FAS) to produce fatty acids and phosphoenol pyruvate carboxykinase (PEPCK-C) to generate glyceride-glycerol through glyceroneogenesis in adipose, and thus increase triglyceride stores (Holland and Hardie, 1985;Chen et al, 2010). However, fasting decreases the concentrations of both insulin and apelin in circulation (Choi et al, 2010;Yue et al, 2010;Viscarra et al, 2011b;Viscarra et al, 2011a;Yue et al, 2011). Therefore, mammals enduring prolonged food deprivation must possess wellregulated intracellular mechanisms to properly manage lipid metabolism to prevent premature depletion of their energy stores.…”

Section: Introductionmentioning

confidence: 99%

Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris)

Viscarra

Vázquez‐Medina

Rodríguez

et al. 2012

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYThe northern elephant seal pup (Mirounga angustirostris) undergoes a 2-3month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. The concentration of non-esterified fatty acids (NEFAs) increases and is associated with the development of insulin resistance in late-fasted pups. Furthermore, plasma NEFA concentrations respond differentially to an intravenous glucose tolerance test (ivGTT) depending on fasting duration, suggesting that the effects of glucose on lipid metabolism are altered. However, elucidation of the lipolytic mechanisms including lipase activity during prolonged fasting in mammals is scarce. To assess the impact of fasting and glucose on the regulation of lipid metabolism, adipose tissue and plasma samples were collected before and after ivGTTs performed on early (2weeks, N5) and late (6-8weeks; N8) fasted pups. Glucose administration increased plasma triglycerides and NEFA concentrations in late-fasted seals, but not plasma glycerol. Fasting decreased basal adipose lipase activity by 50%. Fasting also increased plasma lipase activity twofold and decreased the expressions of CD36, FAS, FATP1 and PEPCK-C by 22-43% in adipose tissue. Plasma acylcarnitine profiling indicated that late-fasted seals display higher incomplete LCFA -oxidation. Results suggest that long-term fasting induces shifts in the regulation of lipolysis and lipid metabolism associated with the onset of insulin resistance in northern elephant seal pups. Delineation of the mechanisms responsible for this shift in regulation during fasting can contribute to a more thorough understanding of the changes in lipid metabolism associated with dyslipidemia and insulin resistance in mammals.

show abstract

Section: Introductionmentioning

confidence: 99%

Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris)

Viscarra

Vázquez‐Medina

Rodríguez

et al. 2012

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Although Akt activation is both necessary and sufficient to transduce the insulin signal to enhance glucose transport in adipocytes (42), we cannot rule out the involvement of alternate signaling pathways, such as atypical protein kinase C (43). Insulin suppresses lipolysis in WAT via both Akt-dependent and Akt-independent signaling pathways through HSL or perilipin, respectively (44). We did (Fig.…”

Section: Discussionmentioning

confidence: 70%

Systemic VEGF-A Neutralization Ameliorates Diet-Induced Metabolic Dysfunction

Meoli

Mangiafico

et al. 2014

Diabetes

View full text Add to dashboard Cite

The vascular endothelial growth factor (VEGF) family of cytokines are important regulators of angiogenesis that have emerged as important targets for the treatment of obesity. While serum VEGF levels rise during obesity, recent studies using genetic models provide conflicting evidence as to whether VEGF prevents or accelerates metabolic dysfunction during obesity. In the current study, we sought to identify the effects of VEGF-A neutralization on parameters of glucose metabolism and insulin action in a dietary mouse model of obesity. Within only 72 h of administration of the VEGF-A–neutralizing monoclonal antibody B.20-4.1, we observed almost complete reversal of high-fat diet–induced insulin resistance principally due to improved insulin sensitivity in the liver and in adipose tissue. These effects were independent of changes in whole-body adiposity or insulin signaling. These findings show an important and unexpected role for VEGF in liver insulin resistance, opening up a potentially novel therapeutic avenue for obesity-related metabolic disease.

show abstract

“…Karakteristik DM yang lain adalah hambatan lipogenesis dan lipolisis yang berlebihan akibat defisiensi atau resistensi terhadap insulin. 3 Berbagai macam terapi telah ditemukan untuk mengontrol kadar gula darah pada pasien DM. Kontrol gula darah dengan baik akan mencegah kejadian komplikasi penyakit DM.…”

Section: Pendahuluanunclassified

EKSTRAK DAUN Nicotiana tabacum MENURUNKAN BERAT LEMAK VISCERAL PADA TIKUS DIABETES MELLITUS TIPE DUA

Suprapto

Jonathan²,

Ernanda³

et al. 2017

View full text Add to dashboard Cite

AbstrakDiabetes mellitus merupakan masalah utama kesehatan di dunia dan prevalensinya selalu meningkat. Pada dosis yang tepat kandungan nikotin dari ekstrak etanol daun tembakau (Nicotiana tabacum) memiliki efek hipoglikemik sehingga berpeluang sebagai terapi alternatif untuk diabetes mellitus. Namun, efeknya pada jaringan adipose penderita diabetes mellitus belum banyak diketahui. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol daun tembakau terhadap berat lemak visceral tikus model diabetes mellitus. Pada penelitian ini tikus galur Wistar dibagi menjadi 5 kelompok yaitu kontrol negatif (K-), kelompok tikus model diabetes mellitus (K+), dan tiga kelompok tikus diabetes melitus yang diberi ekstrak etanol daun tembakau 90 (P1), 180 (P2) dan 270 (P3) mg/kgBB/hari per oral selama 28 hari. Hasil penelitian menunjukkan bahwa terdapat penurunan bermakna berat lemak pada semua kelompok tikus DM yang diberi ekstrak daun tembakau dibandingkan dengan tikus DM tanpa terapi maupun tikus normal (p < 0,05). Kesimpulan penelitian adalah pemberian ekstrak etanol daun tembakau menurunkan berat lemak visceral pada tikus model DM. Penurunan berat lemak visceral kemungkinan disebabkan oleh kandungan nikotin dalam ekstrak yang dapat mengaktivasi lipolisis, menghambat lipogenesis, aktivasi thermogenesis, dan penurunan intake makan.Kata kunci: berat lemak visceral, diabetes mellitus, daun tembakau (Nicotiana tabacum). ETHANOL EXTRACT OF Nicotiana tabacum REDUCES THE VISCERAL WEIGHT OF TYPE TWO DIABETIC RATS AbstractDiabetes melllitusis the main health problem in the world and its prevalence is increasing dramatically. Tobacco leaf (Nicotiana tabacum) containing nicotine could be used as an alternative treatment for diabetes mellitus at the right dose. However, its effect on adipose tissue of diabetes mellitus model has not established well. This study aim is to observe the effect of ethanol extract of tobacco leaf to the visceral weight of diabetic rats. Rats were divided into five group normal rats (K-), diabetic rats (K+) and diabetic rats receiving ethanol extract of tobacco leaf at dose 90 (P1), 80 (P2), and 270 (P3) mg/kgBW/day orally for 28 days. Rats were sacrificed and visceral fat was weighed afterward. As the result, visceral fat of diabetic rats was less than the normal rats and diabetic rats treated with ethanol extract of tobacco leaf shown to reduce visceral weight significantly compared to normal rats (p < 0.05). Visceral weight decrement was due to nicotine effect that could induce lipolysis, inhibit lipogenesis, thermogenesis activation, and reduce food intake. Further research is needed to explore Nicotiana tabacum as an alternative treatment for diabetes mellitus.

show abstract

Insulin Regulates Adipocyte Lipolysis via an Akt-Independent Signaling Pathway

Cited by 182 publications

References 73 publications

Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris)

Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris)

Systemic VEGF-A Neutralization Ameliorates Diet-Induced Metabolic Dysfunction

EKSTRAK DAUN Nicotiana tabacum MENURUNKAN BERAT LEMAK VISCERAL PADA TIKUS DIABETES MELLITUS TIPE DUA

Contact Info

Product

Resources

About