Insulin Receptor Gene Expression in Normal and Diseased Bovine Liver

Liu, G.W.; Zhang, Zhigang; Wang, J.G.; Wang, Z.; Xu, Chuang; Zhu, Xiaoyan

doi:10.1016/j.jcpa.2010.04.004

Cited by 14 publications

(17 citation statements)

References 14 publications

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“…Someone observed [5,7] that the active concentration of oxidant is consistent with peak value of MDA, it is considered as an adaptation phenomenon due to an increased oxidative stress. Wang zhe et al reported that total antioxidative ability changed nonsignificantly in dairy cows affected ketosis, and mRNA expression of insulin receptor decreased [16,17]. There has always been no standardization to evaluate in a broken balance between oxidation and anti-oxidation in dairy cows with ketosis, so it is difficult to offer some detailed evidences on relationship of oxidative stress and ketosis [6,8].…”

Section: Discussionmentioning

confidence: 99%

Investigation on the Relationship of Insulin Resistance and Ketosis in Dairy Cows

Shu¹

2014

J Veterinar Sci Technol

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Section: Discussionmentioning

confidence: 99%

Investigation on the Relationship of Insulin Resistance and Ketosis in Dairy Cows

Shu¹

2014

J Veterinar Sci Technol

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“…The relative abundance of InsR on a cell is regulated by a balance between synthesis and degradation, which can be influenced by various physiological and pathological factors [9,20,21]. Impaired InsR signaling will result in impaired insulin-stimulated glucose uptake and decreased hepatic glucose production [22].…”

Section: Discussionmentioning

confidence: 99%

“…The expression of InsR mRNA in the liver of ketotic dairy cows and cows with fatty liver was reported to be lower than that in the liver of healthy cows, and might be due to insulin resistance [9]. Insulin is an anabolic hormone that plays numerous roles in the metabolism of carbohydrates, lipids and proteins, acts to preserve nutrient availability, and is a potent regulator of feed intake regulator [28].…”

Section: Discussionmentioning

confidence: 99%

High Insulin Concentrations Repress Insulin Receptor Gene Expression in Calf Hepatocytes Cultured <i>in Vitro</i>

Zhang

Liu

et al. 2011

Cell Physiol Biochem

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The major role of insulin and the insulin receptor (InsR) in the liver is to mediate glucose uptake into hepatocytes to synthesize glycogen and to maintain blood glucose homeostasis. In this study, we investigated the effects of high insulin concentrations on InsR gene expression in calf hepatocytes cultured in vitro. After the cells were cultured for 72 h, insulin was added to the culture solution at final concentrations of 0, 1, 10, 100 or 1000 nM. InsR mRNA expression was determined by semi-quantitative RT-PCR. The results showed that InsR mRNA expression in hepatocytes, adjusted for β-actin expression, decreased dose dependently with increasing insulin concentration. InsR mRNA expression was similar at 1 and 10 nM insulin, but was significantly lower than that in the control. InsR expression was similar at 100 and 1000 nM insulin, but was significantly lower than that in the control, 1 and 10 nM insulin groups. These data suggest that high concentrations of insulin significantly repress InsR mRNA expression in calf hepatocytes, and this inhibition occurs in a dose-dependent manner. Further studies are needed to investigate the mechanisms underlying these effects of insulin.

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“…Overfeeding during the dry period has been shown to increase hepatic IL1B mRNA expression (Janovick-Guretzky et al, 2007). Nutrition, immune function and dairy cattle Furthermore, Liu et al (2010) observed lower hepatic insulin receptor mRNA expression for cows with ketosis and hepatic lipidosis when compared with normal cows indicating that responses to insulin are decreased for cows suffering from a metabolic disease. These studies may indicate why some cows fail to adapt to the metabolic demands around the periparturient period and encourage future research studies linking insulin resistance to risk of PI and the development of infectious and non-infectious diseases during the periparturient period.…”

Section: Strategies For Preventing Disease During Early Lactationmentioning

confidence: 96%

Nutrition, immune function and health of dairy cattle

Ingvartsen

Moyes

2013

Animal

264

214

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The large increase in milk yield and the structural changes in the dairy industry have caused major changes in the housing, feeding and management of the dairy cow. However, while large improvements have occurred in production and efficiency, the disease incidence, based on veterinary records, does not seem to be improved. Earlier reviews have covered critical periods such as the transition period in the cow and its influence on health and immune function, the interplay between the endocrine system and the immune system and nutrition and immune function. Knowledge on these topics is crucial for our understanding of disease risk and our effort to develop health and welfare improving strategies, including proactive management for preventing diseases and reducing the severity of diseases. To build onto this the main purpose of this review will therefore be on the effect of physiological imbalance (PI) on immune function, and to give perspectives for prevention of diseases in the dairy cow through nutrition. To a large extent, the health problems during the periparturient period relate to cows having difficulty in adapting to the nutrient needs for lactation. This may result in PI, a situation where the regulatory mechanisms are insufficient for the animals to function optimally leading to a high risk of a complex of digestive, metabolic and infectious problems. The risk of infectious diseases will be increased if the immune competence is reduced. Nutrition plays a pivotal role in the immune response and the effect of nutrition may be directly through nutrients or indirectly by metabolites, for example, in situations with PI. This review discusses the complex relationships between metabolic status and immune function and how these complex interactions increase the risk of disease during early lactation. A special focus will be placed on the major energetic fuels currently known to be used by immune cells (i.e. glucose, non-esterified fatty acids, beta-hydroxybutyrate and glutamine) and how certain metabolic states, such as degree of negative energy balance and risk of PI, contribute to immunosuppression during the periparturient period. Finally, we will address some issues on disease prevention through nutrition.

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Insulin Receptor Gene Expression in Normal and Diseased Bovine Liver

Cited by 14 publications

References 14 publications

Investigation on the Relationship of Insulin Resistance and Ketosis in Dairy Cows

Investigation on the Relationship of Insulin Resistance and Ketosis in Dairy Cows

High Insulin Concentrations Repress Insulin Receptor Gene Expression in Calf Hepatocytes Cultured <i>in Vitro</i>

Nutrition, immune function and health of dairy cattle

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