Insulin-like plant proteins as potential innovative drugs to treat diabetes—The Moringa oleifera case study

Paula, Paulo Carvalho de; Oliveira, José T.A.; Sousa, Daniele O.B.; Alves, Brayan; Carvalho, Ana Fontenele Urano; Franco, Octávio L.; Vasconcelos, Ilka M.

doi:10.1016/j.nbt.2016.10.005

Cited by 22 publications

(14 citation statements)

References 104 publications

(120 reference statements)

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“…Furthermore, insulin-like plant proteins may partly attribute to the relation. In several vitro and in vivo studies, insulin-like plant proteins was isolated from leave, seeds and fruits, which was proven to increase tolerance to orally administered glucose with hypoglycemic activity in chemically induced diabetic mice [ 38 – 40 ].…”

Section: Discussionmentioning

confidence: 99%

Association between dietary protein intake and type 2 diabetes varies by dietary pattern

Qin

Chen

et al. 2018

Diabetol Metab Syndr

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BackgroundEpidemiological studies have demonstrated that high total protein intake was related to type 2 diabetes mellitus (T2DM) risks. However, few studies considered the impact of dietary pattern.ObjectiveWe examined the associations between protein intake and T2DM in different dietary patterns.MethodsWe used the demographic and dietary information of adults aged 18–75 years from the China Health and Nutrition Survey (2009), consisting of 4113 women and 4580 men. Dietary data was collected by using 24-h recalls combined with a food inventory for 3 consecutive days. Cluster analysis was used to classify subjects into groups, as determined by major sources of protein. Logistic regression models were used to calculate odds ratios (OR) and 95% confidence interval (95% CI) of T2DM according to the energy-adjusted protein intake.ResultsAll participants were divided into three patterns according to the dietary source of protein (legumes and seafood, red meat, refined grains). Overall, plant protein intake was significantly and inversely associated with T2DM. In the subgroup analysis by dietary patterns, extreme quartile of plant protein intake was also inversely related to T2DM in the “legumes and seafood” group [OR = 0.58, 95% CI (0.33–0.96)]. Total protein intake and animal protein intake were positively related to T2DM in the “red meat” group [OR: 3.12 (1.65–5.91) and 3.48 (1.87–6.60), respectively]. However, the association of animal protein intake was reversed in the “refined grains” group [OR = 0.55, 95% CI 0.32–0.89].ConclusionsThe association between protein intake and T2DM varies by dietary pattern. Dietary pattern may be considered into the recommendation of protein intake for diabetes prevention.

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Section: Discussionmentioning

confidence: 99%

Association between dietary protein intake and type 2 diabetes varies by dietary pattern

Qin

Chen

et al. 2018

Diabetol Metab Syndr

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“…Several plant lectins show similar behavior [ 26 , 27 ]. The importance of detecting lectins in Mo -LPI relies on the fact that there are some reports of hypoglycemic activity in vivo of plant hemagglutinins [ 9 , 10 , 11 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Plant secondary metabolites are usually extracted using specific solvents and protocols and during these processes, most proteins lose their structural integrity and activity [ 19 , 20 ]. To the best of our knowledge, there is only one work done on the hypoglycemic activity of proteins from M. oleifera [ 21 ]. In this study carried out by our research group, a protein fraction obtained from the seed coat of M. oleifera , named Mo -SC, promoted a substantial reduction in the glucose levels of alloxan-induced diabetic mice after intraperitoneal or oral administration.…”

Section: Introductionmentioning

confidence: 99%

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice

et al. 2017

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Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes.

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“…The extract reduced creatinine and urea levels from damaged kidneys and increased the immune tolerance of the diabetic rats by increasing the activity of CD69, INF-γ, and CD44. Insulin-like protein was observed in the seed coat of M. oleifera (Paula et al, 2016 ). This protein had antigenic epitopes similar to insulin and displayed hypoglycemic activity on oral administration.…”

Section: Biological Activitiesmentioning

confidence: 99%

Moringa Genus: A Review of Phytochemistry and Pharmacology

2018

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Moringa is a genus of medicinal plants that has been used traditionally to cure wounds and various diseases such as colds and diabetes. In addition, the genus is also consumed as a source of nutrients and widely used for purifying water. The genus consists of 13 species that have been widely cultivated throughout Asia and Africa for their multiple uses. The purpose of this review is to provide updated and categorized information on the traditional uses, phytochemistry, biological activities, and toxicological research of Moringa species in order to explore their therapeutic potential and evaluate future research opportunities. The literature reviewed for this paper was obtained from PubMed, ScienceDirect, and Google Scholar journal papers published from 1983 to March 2017. Moringa species are well-known for their antioxidant, anti-inflammatory, anticancer, and antihyperglycemic activities. Most of their biological activity is caused by their high content of flavonoids, glucosides, and glucosinolates. By documenting the traditional uses and biological activities of Moringa species, we hope to support new research on these plants, especially on those species whose biological properties have not been studied to date.

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Insulin-like plant proteins as potential innovative drugs to treat diabetes—The Moringa oleifera case study

Cited by 22 publications

References 104 publications

Association between dietary protein intake and type 2 diabetes varies by dietary pattern

Association between dietary protein intake and type 2 diabetes varies by dietary pattern

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice

Moringa Genus: A Review of Phytochemistry and Pharmacology

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