Insulin-Like Growth Factor Type I (IGF-I) Supports Growth of V-relER Dendritic Cell Progenitors

Madruga, Jaime; Koritschoner, Nicolás P.; Stengl, Gabi; Knespel, Signe; Bartůněk, Petr; Zenke, Martin

doi:10.1007/978-1-4757-9966-8_76

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“…Basically, these strategies employed primers directed to highly conserved sequences anking the region that encodes the catalytic domain and that is homologous in a number of tyrosine kinases (Hanks et al, 1988). Such studies identi®ed novel tyrosine kinases as well as determined tyrosine kinase gene expression in various cell populations (Partanen et al, 1990;Hovens et al, 1992;Marcelle and Eichmann, 1992;Choi et al, 1993Choi et al, , 1994Tamagnone et al, 1993;Yee et al, 1993;Simoneaux et al, 1995;Hoehn et al, 1996;Madruga et al, 1997;Robinson et al, 1998). However, in many instances further analysis of the tyrosine kinases identi®ed and of their biological activity remained di cult, mainly due to limitations in obtaining homogenous cell populations to follow up candidate genes.…”

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confidence: 99%

The fibroblast growth factor receptor FGFR-4 acts as a ligand dependent modulator of erythroid cell proliferation

Bartůněk

Knespel

et al. 1999

Oncogene

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Receptor and non-receptor tyrosine kinases constitute a large family of proteins that play a pivotal role in hematopoiesis. Here we conducted a comprehensive survey of tyrosine kinase gene expression in primary erythroid progenitor cells from bone marrow by employing a PCR-based strategy that targets the conserved kinase encoding region. We demonstrate that erythroid progenitor cells express several receptor and nonreceptor tyrosine kinases, like c-kit, Jak1, Ryk, FAK, Syk, Arg, Csk and members of the insulin receptor family. Speci®c changes in the expression pro®le of tyrosine kinases were observed following di erentiation induction. We also report on the identi®cation of a new ligand dependent modulator of erythropoiesis, ®broblast growth factor receptor-4 (FGFR-4). FGFR-4 is e ectively expressed in erythroid progenitors and downregulated when cells di erentiate. Furthermore, the FGFR-4 ligand, basic ®broblast growth factor (bFGF), enhanced erythroid cell proliferation induced by SCF or insulin, and thus modulated both erythroid proliferation and di erentiation in vitro.

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