The fibroblast growth factor receptor FGFR-4 acts as a ligand dependent modulator of erythroid cell proliferation

Np, Koritschoner; Bartůněk, Petr; Knespel, Signe; Blendinger, Gitta; Zenke, Martin

doi:10.1038/sj.onc.1202979

Cited by 20 publications

(17 citation statements)

References 69 publications

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“…bFGF was however reported to be important for proliferation of hemangioblast, a common progenitor of endothelial and hematopoietic cells (Faloon et al, 2000). Additionally, bFGF was found in our previous studies to synergize with other growth factors in inducing proliferation of primitive uncommitted progenitors and de®nitive erythroid cells (Koritschoner et al, 1999). We demonstrate here that bFGF is a key factor for proliferation and survival of primitive erythroid progenitors and thus in fact represents a hematopoietic growth factor.…”

Section: Discussionmentioning

confidence: 58%

“…Our previous work demonstrated that bFGF cooperates with various growth factors in the development of de®nitive erythroid progenitor cells (Koritschoner et al, 1999). Additionally, bFGF alone was found to be ine ective in supporting progenitor cell growth but caused sustained growth of immature progenitors derived from blastoderm that expressed elevated levels of v-Myb (Kara®at et al, 2001).…”

Section: Bfgf Causes Growth Of Primitive Erythroid Cells Containing Vmentioning

confidence: 99%

“…Blots were stained with Methylene Blue to demonstrate equal RNA loading per lane. Probes correspond to cDNA of chicken FGFR1, FGFR2, FGFR3 (Cek1, Cek3 and Cek2, respectively; Pasquale, 1990) and chicken FGFR4 (Koritschoner et al, 1999).…”

Section: Rna Preparation and Northern Blot Analysismentioning

confidence: 99%

“…Furthermore, genetic mutations of SCF and SCF receptor in Steel (SI) and Dominant white spotting (WW) loci, respectively, were associated with severe anemia (Broudy, 1997). Other factors such as basic ®broblast growth factor (bFGF, FGF-2), transforming growth factor a (TGFa) and members of the TGFb family, including TGFb, bone morphogenic proteins (BMPs) and activin A, are also involved (Krystal et al, 1994;Dickson et al, 1995;Huber et al, 1998;Mead and Zon, 1998;Gandrillon et al, 1999;Koritschoner et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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bFGF signaling and v-Myb cooperate in sustained growth of primitive erythroid progenitors

Bartůněk¹,

Pajer²,

Karafiát³

et al. 2002

Oncogene

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The development of red blood cells from hematopoietic progenitors requires the interplay of speci®c extracellular factors and transcriptional regulators. Here we have identi®ed an erythroid progenitor that is critically dependent on bFGF and requires expression of AMV v-Myb for sustained proliferation in vitro, indicating that bFGF and Myb proteins cooperate in these cells. In the presence of bFGF such v-Myb cells are completely blocked in their ability to di erentiate and exhibit an exceptionally high proliferative potential and long lifespan in vitro. Interestingly, in the absence of bFGF cells e ectively di erentiate into mature erythrocytes, irrespective of constitutive and elevated levels of v-Myb. We also demonstrate that these cells express high levels of FGF receptor type 1 (FGFR1) and that phospholipase Cg (PLCg) is one of the important molecules in FGF receptor signaling. Our studies suggest that bFGF, in cooperation with Myb proteins, represents an important factor for determining erythroid lineage choice. These ®ndings unravel a so far unidenti®ed link between extracellular signaling and Myb in hematopoietic cells.

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Section: Discussionmentioning

confidence: 58%

Section: Bfgf Causes Growth Of Primitive Erythroid Cells Containing Vmentioning

confidence: 99%

Section: Rna Preparation and Northern Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

bFGF signaling and v-Myb cooperate in sustained growth of primitive erythroid progenitors

Bartůněk¹,

Pajer²,

Karafiát³

et al. 2002

Oncogene

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“…The role of FGFR4 in carcinogenesis is poorly understood. FGFR4 mediates membrane ruffling in breast carcinoma cells (9), where it is overexpressed (10), and it has been shown to modulate erythroid cell proliferation (11). A tumor-derived FGFR4 (ptd-FGFR4) isoform recapitulates pituitary tumorigenesis in transgenic mice (12).…”

Section: Introductionmentioning

confidence: 99%

Sp1-Mediated Transcriptional Control of Fibroblast Growth Factor Receptor 4 in Sarcomas of Skeletal Muscle Lineage

Yu¹,

Ladanyi²,

Sylvia³

et al. 2004

Clinical Cancer Research

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Fibroblast growth factor receptors (FGFRs) have been implicated in a multitude of differentiating and proliferative actions. FGFR4 is expressed mainly in lung, kidney, pancreas, spleen, and developing muscle. FGFR4 was found to be overexpressed in some human malignancies, where it has been implicated in their pathogenesis. Recently, FGFR4 was found to be overexpressed in pediatric rhabdomyosarcomas, based on cDNA microarray analysis. Using Northern blotting, reverse transcription-polymerase chain reaction, and Western blotting, we classified four human rhabdomyosarcoma-derived cell lines based on their relative expression of FGFR4. We defined a 214 bp (؊115/؉99) promoter that functioned as a minimal promoter and examined cis-DNA elements implicated in the control of expression of the FGFR4 gene in these cells. Overlapping 40-to 50-bp fragments of the minimal promoter were examined by electrophoretic mobility shift assay using nuclear extracts from cell lines with high (HS729-1015)

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The impact of c-met/scatter factor receptor on dendritic cell migration

et al. 2002

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Dendritic cells (DC) are professional antigen‐presenting cells that possess both migratory properties and potent T cell stimulatory activity, and that allow the uptake of antigenic material inperipheral tissues and its subsequent presentation in the T cell areas of lymphoid organs. Thus motility represents a central property that is required for DC function. Here we report on the expression of the receptor tyrosine kinase c‐met in DC. c‐Met is the high affinity receptor for scatter factor (SF)/hepatocyte growth factor, and ligand‐activated c‐met exhibits mitogenic, morphogenic andmotogenic activity in vivo and in vitro. c‐Met is signaling competent in DC since it is effectively tyrosine phosphorylated in response to SF ligand. It is demonstrated here that ligand‐activated c‐met regulates DC adhesion to the extracellular matrix component laminin but leaves antigen presenting function unaffected. Importantly, in ear sheet explant experiments activationof c‐met by ligand induces emigration of cutaneous DC (Langerhans cell, LC) from skin, but SF is not a chemoattractant factor for DC. Our results suggest an important role of the c‐met/SF system in DC/LC migration.

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The fibroblast growth factor receptor FGFR-4 acts as a ligand dependent modulator of erythroid cell proliferation

Cited by 20 publications

References 69 publications

bFGF signaling and v-Myb cooperate in sustained growth of primitive erythroid progenitors

bFGF signaling and v-Myb cooperate in sustained growth of primitive erythroid progenitors

Sp1-Mediated Transcriptional Control of Fibroblast Growth Factor Receptor 4 in Sarcomas of Skeletal Muscle Lineage

The impact of c-met/scatter factor receptor on dendritic cell migration

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