Insulin-like growth factor binding protein 5 (IGFBP5) functions as a tumor suppressor in human melanoma cells

Wang, Junyun; Ding, Nan; Li, Yongjun; Cheng, Hua; Wang, Dong; Yang, Qiong; Deng, Yongbo; Yang, Yaran; Liu, Yanming; Ruan, Xiuhang; Xie, Fang; Zhao, Hua; Fang, Xiangdong

doi:10.18632/oncotarget.4114

Cited by 77 publications

(82 citation statements)

References 55 publications

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“…SOX2, CD133 and Nestin have been reported as cancer stem cell markers in melanoma (19)(20)(21). In the present study, the tumor cells of undifferentiated MM expressed CD133 and SOX2.…”

Section: Discussionsupporting

confidence: 64%

Undifferentiated sinonasal malignant melanoma: A case report

Huang

et al. 2018

Oncol Lett

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Undifferentiated sinonasal malignant melanoma (MM) is a rare type of tumor, which can be easily misdiagnosed. The present study reports a 41-year-old male patient who presented with a 4-day history of epistaxis. Clinical examination and radiological imaging lead to the detection of a mass in the right sinonasal region. Histopathological examination revealed that the mass was composed of malignant epithelioid cells arranged in nests and sheets. These cells displayed a hemangiopericytoma-like pattern with antler-like branching vessels. Immunohistochemical staining revealed that the tumor cells exhibited negative expression of melanocytic markers. This increased the difficulty of distinguishing undifferentiated MM from other malignant tumors located in the sinonasal area, particularly undifferentiated nasopharyngeal carcinoma. The diagnosis of undifferentiated MM was determined by ultrastructures, including the mature melanosomes and premelanosomes, in tumor cells by transmission electron microscopy. The present study suggests that the analysis of cancer stem cell marker and vasculogenic mimicry may be an important auxiliary tool for the diagnosis of MM.

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“…SOX2, CD133 and Nestin have been reported as cancer stem cell markers in melanoma (19)(20)(21). In the present study, the tumor cells of undifferentiated MM expressed CD133 and SOX2.…”

Section: Discussionsupporting

confidence: 64%

Undifferentiated sinonasal malignant melanoma: A case report

Huang

et al. 2018

Oncol Lett

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“…Recent studies have demonstrated that IGFBP5 might be a useful biomarker for cancerous tissue and metastasis [31–37], but it also shows the opposite effects depending on cancer cell type and expression method [30, 43–46]. In colon cancer, a study using the rat model revealed that IGFBP5 is one of the top upregulated genes [35].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5

Lü

Han

et al. 2016

Stem Cell Res Ther

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BackgroundColorectal cancer (CRC) is one of the most common malignancies in the world. microRNA-140-5p (miR-140) has been shown to be involved in cartilage development and osteoarthritis (OA) pathogenesis. Some contradictions still exist concerning the role of miR-140 in tumor progression and metastasis, and the underlying mechanism is uncertain.MethodsImmunohistochemistry was performed to determine the expressions of ADAMTS5 and IGFBP5 in CRC tissues. Human CRC cell lines HCT116 and RKO were transfected with miR-140 mimic, inhibitor, or small interfering RNA (siRNA) against ADAMTS5 or IGFBP5, respectively, using oligofectamine or lipofectamine 2000. Scratch-wound assay and transwell migration and invasion assays were used to evaluate the effects of miR-140 on the capabilities of migration and invasion. The levels of miR-140 and ADAMTS5 and IGFBP5 mRNA were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was performed to examine the expression of ADAMTS5 and IGFBP5 proteins.ResultsmiR-140 was significantly reduced, whereas ADAMTS5 and IGFBP5 were upregulated, in the human CRC tissues compared to the corresponding normal colorectal mucosa. miR-140 downregulation and ADAMTS5 or IGFBP5 overexpression were associated with the advanced TNM stage and distant metastasis of CRC. There was a reverse correlation between miR-140 levels and ADAMTS5 and IGFBP5 expression in CRC tissues. ADAMTS5 and IGFBP5 were downregulated by miR-140 at both the protein and mRNA levels in the CRC cell lines. The gain-of- and loss-of-function studies showed that miR-140 inhibited CRC cell migratory and invasive capacities at least partially via downregulating the expression of ADAMTS5 and IGFBP5.ConclusionsThese findings suggest that miR-140 suppresses CRC progression and metastasis, possibly through downregulating ADAMTS5 and IGFBP5. miR-140 might be a potential therapeutic candidate for the treatment of CRC.

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“…IGFBP‐3 and ‐5 are the massive and conserved secreted proteins in the family of IGFBPs, which could transfer to the nucleus through a nuclear localization sequence . It has been implicated that dysregulated IGFBP5 may involve the carcinogenesis and progression of several carcinomas, for example, oral carcinoma and breast carcinoma . More importantly, IGFBP6 has been verified to be a new causal variant of DD .…”

Section: Introductionmentioning

confidence: 99%

Overexpressed IGFBP5 promotes cell proliferation and inhibits apoptosis of nucleus pulposus derived from rats with disc degeneration through inactivating the ERK/MAPK axis

Wang

Shen

et al. 2019

J of Cellular Biochemistry

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It is previously suggested that insulin‐like growth factor binding proteins (IGFBPs) potentially share an association with disc degeneration (DD) that causes back pain. This study aimed at exploring the functional relevance of IGFBP5 in DD by establishing a rat model of DD. The nucleus pulposus (NP) cells were transduced with IGFBP5‐shRNA or IGFBP5 overexpression to determine the cellular processes (proliferation, apoptosis, as well as colony formation). The protein levels of apoptosis‐related proteins were evaluated. Furthermore, NP cells were treated with the extracellular signal‐regulated kinases/mitogen‐activated protein kinase (ERK/MAPK) pathway inhibitor (PD98059) followed by measurement of ERK protein level and ERK phosphorylation content. The NP cells showed suppressed proliferation and colony formation ability, yet promoted apoptosis after transfection with IGFBP5‐shRNA. It was found that silencing of IGFBP5 could lead to the ERK/MAPK axis activation, as indicated by an elevated ERK protein level and ERK phosphorylation content. However, overexpression of IGFBP5 could reverse all the reaction induced by silenced IGFBP5. These key findings demonstrate that overexpressed IGFBP5 inactivates the ERK/MAPK axis to stimulate the proliferation and inhibit apoptosis of NP cells in a rat model of DD.

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Insulin-like growth factor binding protein 5 (IGFBP5) functions as a tumor suppressor in human melanoma cells

Cited by 77 publications

References 55 publications

Undifferentiated sinonasal malignant melanoma: A case report

Undifferentiated sinonasal malignant melanoma: A case report

RETRACTED ARTICLE: microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5

Overexpressed IGFBP5 promotes cell proliferation and inhibits apoptosis of nucleus pulposus derived from rats with disc degeneration through inactivating the ERK/MAPK axis

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