Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Kawano, Yasushi; Narahara, Hisashi; Matsui, Noriko; Nasu, Kaei; Miyamura, Kenji; Miyakawa, Isao

doi:10.1159/000291507

Cited by 23 publications

(20 citation statements)

References 18 publications

(23 reference statements)

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“…A large number of cytokines, growth factors, and related proteins have been identified in human FF and in the ovary [3,4,[17][18][19]. These factors may exert local effects upon steroidogenesis, granulosa cell proliferation, follicular growth, and gonadotropin receptor concentration.…”

Section: Discussionmentioning

confidence: 99%

The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids

Kawasaki¹,

Kawano²,

Hasan³

et al. 2003

Clinical and Experimental Medicine

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In order to investigate the role of interleukin-6 (IL-6) and interleukin-6 soluble receptor (sR) in human ovulation, we evaluated the concentrations in human follicular fluid and analyzed the correlation of IL-6 and IL-6 sR with oocyte maturation. The oocytes were obtained from the follicular fluid of 45 women undergoing in vitro fertilization and embryo transfer. The concentrations of IL-6 and IL-6 sR in follicular fluid were measured by ELISA. In addition, granulosa cells obtained from the follicular fluid were cultured and treated with forskolin and 12- o-tetradecanoylphorbol 13-acetate for 24-48 h. The concentration of IL-6 was significantly higher in the follicular fluid than in the serum (P<0.01). In contrast, the concentration of IL-6 sR was significantly lower in the follicular fluid than in the serum (P<0.001). The concentrations of IL-6 and IL-6 sR were significantly higher in the follicular fluid containing mature oocytes than in fluid containing immature oocytes (P<0.05). The production of IL-6 was markedly increased over the basal level after 24 h of treatment with forskolin (P<0.001) and 48 h of treatment (P<0.01) with cultured granulosa cells. Our data suggest that IL-6 and IL-6 sR may play an important role in follicular growth and development in human preovulatory processes. It is possible that IL-6 in particular may be regulated by cAMP. IL-6 and IL-6 sR might also be valuable biochemical markers in the evaluation of oocyte maturation.

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Section: Discussionmentioning

confidence: 99%

The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids

Kawasaki¹,

Kawano²,

Hasan³

et al. 2003

Clinical and Experimental Medicine

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“…These findings suggest that IGFBP-3 may play a significant role in oocyte maturati on in the gonadotropin-induced ovulatory process. In contrast, the concentrations of IGFBP-1 in follicular fluids with mature oocytes are significantly higher than those in follicular fluids with intermediate or immature oocytes [16]. Significantly p o s i t i v e c o r r e l a t i o n s a r e f o u n d b e t w e e n t h e concentrations of IGFBP-1 and E2 and P in follicular fluids.…”

Section: Factors Affecting Oocyte Maturationmentioning

confidence: 81%

“…In addition, IGFBP-3 inhibits IGF-I-induced oocyte maturation in a dose-dependent manner, suggesting that IGFBP-3 may exert an inhibitory signal for IGF-Ii n d u c e d o o c y t e m a t u r a t i o n . I n h u m a n s , t h e concentrations of IGFBP-3 in follicular fluids decrease as oocytes progress to mature [16]. These findings suggest that IGFBP-3 may play a significant role in oocyte maturati on in the gonadotropin-induced ovulatory process.…”

Section: Factors Affecting Oocyte Maturationmentioning

confidence: 89%

Oocyte Maturation in Humans: the Potential Relevance to Reproductive Medicine

Yoshimura

2003

J.Mamm.Ova.Res.

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“…Many cytokines, growth factors and related proteins have been identified in human follicular fluids and in the ovary [29,33,34,35]. These factors may have local effects upon steroidogenesis, granulosa cell proliferation, follicular growth and gonadotropin receptor concentration.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Epidermal Growth Factor and Transforming Growth Factor-a on Secretion of Interleukin-8 and Growth-Regulated Oncogene-a in Human Granulosa-Lutein Cells

Kawano

Zeineh

Furukawa

et al. 2012

Gynecol Obstet Invest

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Background: In order to investigate the roles of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in ovulation, we studied the production of interleukin (IL)-8 and growth-regulated oncogene (GRO)-α in cultured human granulosa-lutein cells. Methods: Granulosa-lutein cells obtained from the follicular fluids of in vitro fertilization and embryo transfer patients were cultured and treated with EGF, TGF-α, tumor necrosis factor (TNF)-α or 12-O-tetradecanoylphorbol 13-acetate (TPA). An immortalized granulosa cell line (GC1a) was also cultured and treated with EGF, TGF-α or mitogen-activated protein kinase kinase inhibitor. The supernatants were collected, and IL-8 and GRO-α were measured by ELISA. Results: The levels of IL-8 and GRO-α were significantly increased after treatment with EGF, TGF-α, TNF-α and TPA by primary cultured granulosa-lutein cells. The levels of IL-8 and GRO-α were also significantly increased after treatment with EGF or TGF-α in a dose-dependent manner by GC1a. When GC1a was treated with EGF, TGF-α or U0126, the levels of IL-8 and GRO-α were significantly decreased. Conclusion: Our data indicate that the production of IL-8 and GRO-α is upregulated by EGF and TGF-α. It is suggested that EGF and TGF-α may play an important role in luteinization processes involving IL-8 and GRO-α production.

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Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Cited by 23 publications

References 18 publications

The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids

The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids

Oocyte Maturation in Humans: the Potential Relevance to Reproductive Medicine

The Effects of Epidermal Growth Factor and Transforming Growth Factor-a on Secretion of Interleukin-8 and Growth-Regulated Oncogene-a in Human Granulosa-Lutein Cells

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